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  4. Human Complement component C8 beta chain(C8B) ELISA kit

Human Complement component C8 beta chain(C8B) ELISA kit

  • 中文名称：
    人补体成分C8β链(C8B)酶联免疫试剂盒
  • 货号：
    CSB-EL004195HU
  • 规格：
    96T/48T
  • 价格：
    ￥3600/￥2500
  • 其他：

产品详情

  • 产品描述：

    This Human C8B ELISA Kit was designed for the quantitative measurement of Human C8B protein in serum, plasma, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 2.74 pg/mL-2000 pg/mL and the sensitivity is 1.37 pg/mL.

  • 别名：
    C8b ELISA Kit; CO8B_HUMAN ELISA Kit; Complement component 8 beta polypeptide ELISA Kit; Complement component 8 subunit beta ELISA Kit; Complement component C8 beta chain ELISA Kit
  • 缩写：
  • Uniprot No.：
  • 种属：
    Homo sapiens (Human)
  • 样本类型：
    serum, plasma, tissue homogenates
  • 检测范围：
    2.74 pg/mL-2000 pg/mL
  • 灵敏度：
    1.37 pg/mL
  • 反应时间：
    1-5h
  • 样本体积：
    50-100ul
  • 检测波长：
    450 nm
  • 研究领域：
    Immunology
  • 测定原理：
    quantitative
  • 测定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%        
    Three samples of known concentration were tested twenty times on one plate to assess.    
    Inter-assay Precision (Precision between assays): CV%<10%        
    Three samples of known concentration were tested in twenty assays to assess.      
                   
  • 线性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of human C8B in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.  
    　 Sample Serum(n=4)    
    1:10000 Average % 92    
    Range % 89-98    
    1:20000 Average % 90    
    Range % 84-100    
    1:40000 Average % 95    
    Range % 92-101    
    1:80000 Average % 93    
    Range % 86-98    
  • 回收率：
    The recovery of human C8B spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.  
     
    Sample Type Average % Recovery Range    
    Serum (n=5) 96 85-104    
    EDTA plasma (n=4) 91 88-97    
                   
                   
  • 标准曲线：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.  
     
    pg/ml OD1 OD2 Average Corrected    
    2000 2.556 2.510 2.533 2.442    
    667 1.881 1.816 1.849 1.758    
    222 1.253 1.301 1.277 1.186    
    74 0.908 0.896 0.902 0.811    
    24.7 0.374 0.366 0.370 0.279    
    8.2 0.205 0.195 0.200 0.109    
    2.74 0.141 0.134 0.138 0.047    
    0 0.089 0.093 0.091 　    
  • 数据处理：
  • 货期：
    3-5 working days

产品评价

靶点详情

  • 功能：
    Constituent of the membrane attack complex (MAC) that plays a key role in the innate and adaptive immune response by forming pores in the plasma membrane of target cells.
  • 基因功能参考文献：
    1. The binding specificity between C8 beta and C8 alpha subunits is determined by a cooperative interaction of the N-terminal thrombospondin type 1 module and the membrane attack complex/perforin domain. PMID: 12220191
    2. results indicate that the principal binding site for C9 lies within the MACPF domain of C8alpha; they also suggest this site and the binding sites for C8beta and C8gamma are distinct PMID: 16618117
  • 相关疾病：
    Complement component 8 deficiency, 2 (C8D2)
  • 亚细胞定位：
    Secreted.
  • 蛋白家族：
    Complement C6/C7/C8/C9 family
  • 数据库链接：

    HGNC: 1353

    OMIM: 120960

    KEGG: hsa:732

    STRING: 9606.ENSP00000360281

    UniGene: Hs.391835