Human Complement factor H-related protein 2(CFHR2) ELISA kit

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产品详情

产品描述：

This Human CFHR2 ELISA Kit was designed for the quantitative measurement of Human CFHR2 protein in serum, plasma, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 6.25 ng/mL-400 ng/mL and the sensitivity is 1.56 ng/mL.
别名：

CFHL2 ELISA Kit; CFHR2 ELISA Kit; Complement factor H related protein 2 ELISA Kit; Complement factor H-related 2 ELISA Kit; Complement factor H-related protein 2 ELISA Kit; DDESK59 ELISA Kit; Factor H-related gene 2 ELISA Kit; FHR 2 ELISA Kit; FHR-2 ELISA Kit; FHR2 ELISA Kit; FHR2_HUMAN ELISA Kit; H factor (complement)-like 3 ELISA Kit; H factor like 3 ELISA Kit; H factor like protein 2 ELISA Kit; H factor-like 3 ELISA Kit; H factor-like protein 2 ELISA Kit; HFL3 ELISA Kit
缩写：

CFHR2
Uniprot No.：

P36980
种属：

Homo sapiens (Human)
样本类型：

serum, plasma, tissue homogenates
检测范围：

6.25 ng/mL-400 ng/mL
灵敏度：

1.56 ng/mL
反应时间：

1-5h
样本体积：

50-100ul
检测波长：

450 nm
研究领域：

Immunology
测定原理：

quantitative
测定方法：

Sandwich

精密度：

Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.

线性度：

To assess the linearity of the assay, samples were spiked with high concentrations of human CFHR2 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:100	Average %	91
	Range %	87-95
1:200	Average %	96
	Range %	90-102
1:400	Average %	104
	Range %	98-109
1:800	Average %	92
	Range %	86-98

回收率：

The recovery of human CFHR2 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	101	97-105
EDTA plasma (n=4)	94	87-98

标准曲线：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

功能：

Involved in complement regulation. The dimerized forms have avidity for tissue-bound complement fragments and efficiently compete with the physiological complement inhibitor CFH. Can associate with lipoproteins and may play a role in lipid metabolism.
基因功能参考文献：
1. Studies indicate an association between CFHR2-rs2986127 and acute anterior uveitis (AAU) diagnosis. PMID: 27306586
2. Next-generation sequencing of the CFH region identified putatively functional variants (missense, splice site and indel) on the four common haplotypes. We found no expression of any of the five CFH-related genes in the retina or RPE/Choroid/Sclera, in contrast to the liver, which is the main source of the circulating proteins. [CFHR2] PMID: 27196323
3. Thus CFHR2 likely acts in concert with factor H, as CFHR2 inhibits convertases while simultaneously allowing factor H assisted degradation by factor I. PMID: 24260121
4. A hybrid CFHR2-CFHR5 plasma protein, arising from a chromosomal deletion mutation stabilizes the C3 convertase and reduces factor H-mediated convertase decay. PMID: 24334459
亚细胞定位：

Secreted.
组织特异性：

Expressed by the liver and secreted in plasma.
数据库链接：

HGNC: 4890

OMIM: 600889

KEGG: hsa:3080

STRING: 9606.ENSP00000356385

UniGene: Hs.632450