- A 96-well Assay plate -- The 96-well plate has been pre-coated with anti-human COR antibody.
- Two vails Lyophilized Standard -- Dilute one of the standards at dilution series, read the OD values, and then draw a standard curve.
- Anti-human COR Antibody (100 x concentrate) 1 x 60 μl-- Act as the detection antibody.
- Antibody Diluent 1 x 10 ml -- Dilute the COR antibody solution.
- HRP-conjugate (100 x concentrate) 1 x 120 μl-- Bind to the COR antibody, and HRP catalyzes the substrate A and B to elicit a chromogenic reaction.
- HRP-conjugate Diluent 1 x 20 ml -- Dilute the HRP-conjugate solution.
- Sample Diluent 2 x 20 ml -- Dilute the standard and sample solutions. Wash Buffer (25 x concentrate) 1 x 20 ml -- Wash away unbound or free substances.
- TMB Substrate 1 x 10 ml -- HRP catalyzes the TMB, eliciting a chromogenic reaction.
- Stop Solution 1 x 10 ml -- Stop the color reaction. The solution color immediately turns from blue to yellow.
- Four Adhesive Strips (For 96 wells)
- An Instruction manual
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