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Human Dipeptidase 1(DPEP1) ELISA kit

  • 中文名称：
    人二肽酶1(DPEP1)酶联免疫试剂盒
  • 货号：
    CSB-EL007123HU
  • 规格：
    96T/48T
  • 价格：
    ￥3600/￥2500
  • 其他：

产品详情

  • 产品描述：

    This Human DPEP1 ELISA Kit was designed for the quantitative measurement of Human DPEP1 protein in serum, plasma, tissue homogenates, cell lysates. It is a Sandwich ELISA kit, its detection range is 23.5 pg/mL-1500 pg/mL and the sensitivity is 5.86 pg/mL.

  • 别名：
    Dehydropeptidase-I ELISA Kit; Dipeptidase 1 (renal) ELISA Kit; Dipeptidase 1 ELISA Kit; DPEP1 ELISA Kit; DPEP1_HUMAN ELISA Kit; hRDP ELISA Kit; MBD1 ELISA Kit; MDP ELISA Kit; Microsomal dipeptidase ELISA Kit; RDP ELISA Kit; Renal dipeptidase ELISA Kit
  • 缩写：
    DPEP1
  • Uniprot No.：
  • 种属：
    Homo sapiens (Human)
  • 样本类型：
    serum, plasma, tissue homogenates, cell lysates
  • 检测范围：
    23.5 pg/mL-1500 pg/mL
  • 灵敏度：
    5.86 pg/mL
  • 反应时间：
    1-5h
  • 样本体积：
    50-100ul
  • 检测波长：
    450 nm
  • 研究领域：
    Cancer
  • 测定原理：
    quantitative
  • 测定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%
    Three samples of known concentration were tested in twenty assays to assess.
  • 线性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of human DPEP1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    　SampleSerum(n=4)
    1:1Average %88
    Range %80-93
    1:2Average %97
    Range %91-106
    1:4Average %102
    Range %92-111
    1:8Average %94
    Range %86-99
  • 回收率：
    The recovery of human DPEP1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample TypeAverage % RecoveryRange
    Serum (n=5) 9589-99
    EDTA plasma (n=4)9890-101
  • 标准曲线：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    pg/mlOD1OD2AverageCorrected
    15001.885 1.752 1.819 1.739
    7501.303 1.223 1.263 1.183
    3750.812 0.778 0.795 0.715
    187.50.465 0.453 0.459 0.379
    940.331 0.319 0.325 0.245
    470.194 0.191 0.193 0.113
    23.50.129 0.126 0.128 0.048
    00.080 0.079 0.080 　
  • 数据处理：
  • 货期：
    3-5 working days

产品评价

靶点详情

  • 功能：
    Hydrolyzes a wide range of dipeptides including the conversion of leukotriene D4 to leukotriene E4. Hydrolyzes cystinyl-bis-glycine (cys-bis-gly) formed during glutathione degradation. Possesses also beta lactamase activity and hydrolytically inactivates beta-lactam antibiotics.; Independently of its dipeptidase activity acts as an adhesion receptor for neutrophils recruitement from bloodstream into inflammed lungs and liver.
  • 基因功能参考文献：
    1. The results suggest that DPEP1 promotes cancer metastasis by regulating E-cadherin plasticity and that it might be a potential therapeutic target for preventing the progression of colon cancer. PMID: 26824987
    2. In this study, we present an analysis of Neanderthal introgression at the dipeptidase 1 gene, DPEP1. PMID: 26392408
    3. Dipeptidase 1 has been identified as an excellent marker of high-grade IEN and CRC, and may thus be applied for screening of early neoplastic lesions and for prognostic stratification. PMID: 23839495
    4. DPEP1 plays a role in pancreatic cancer aggressiveness. PMID: 22363658
    5. DPEP1 is expressed in the early stages of colon carcinogenesis and affects cancer cell invasiveness. PMID: 20824289
    6. we identified a novel immunohistochemical marker, dipeptidase 1, to distinguish primary mucinous ovarian cancers from ovarian metastasis of colorectal cancers. PMID: 21076463
    7. Crystal structure of human renal dipeptidase involved in beta-lactam hydrolysis PMID: 12144777
    8. DPEP1 has a role in colorectal carcinoma PMID: 15145522

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  • 亚细胞定位：
    Apical cell membrane; Lipid-anchor, GPI-anchor. Cell projection, microvillus membrane; Lipid-anchor, GPI-anchor.
  • 蛋白家族：
    Metallo-dependent hydrolases superfamily, Peptidase M19 family
  • 组织特异性：
    Expressed in lung and kidneys.
  • 数据库链接：

    HGNC: 3002

    OMIM: 179780

    KEGG: hsa:1800

    STRING: 9606.ENSP00000261615

    UniGene: Hs.109