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  4. Human Epigen(EPGN) ELISA kit

Human Epigen(EPGN) ELISA kit

  • 中文名称：
    人Epigen(EPGN)酶联免疫试剂盒
  • 货号：
    CSB-EL007719HU
  • 规格：
    96T/48T
  • 价格：
    ￥3600/￥2500
  • 其他：

产品详情

  • 别名：
    ALGV3072 ELISA Kit; EPG ELISA Kit; EPGN ELISA Kit; EPGN_HUMAN ELISA Kit; Epigen ELISA Kit; Epigen precursor ELISA Kit; Epithelial mitogen ELISA Kit; PRO9904 ELISA Kit
  • 缩写：
    EPGN
  • Uniprot No.：
  • 种属：
    Homo sapiens (Human)
  • 样本类型：
    serum, plasma, tissue homogenates
  • 检测范围：
    1.56 ng/mL-100 ng/mL
  • 灵敏度：
    0.39 ng/mL
  • 反应时间：
    1-5h
  • 样本体积：
    50-100ul
  • 检测波长：
    450 nm
  • 研究领域：
    Others
  • 测定原理：
    quantitative
  • 测定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%        
    Three samples of known concentration were tested twenty times on one plate to assess.    
    Inter-assay Precision (Precision between assays): CV%<10%        
    Three samples of known concentration were tested in twenty assays to assess.      
                   
  • 线性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of human EPGN in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.  
    　 Sample Serum(n=4)    
    1:1 Average % 103    
    Range % 96-107    
    1:2 Average % 102    
    Range % 97-105    
    1:4 Average % 92    
    Range % 85-96    
    1:8 Average % 97    
    Range % 92-101    
  • 回收率：
    The recovery of human EPGN spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.  
     
    Sample Type Average % Recovery Range    
    Serum (n=5) 97 92-103    
    EDTA plasma (n=4) 105 98-109    
                   
                   
  • 标准曲线：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.  
     
    pg/ml OD1 OD2 Average Corrected    
    100 2.687 2.568 2.628 2.528    
    50 2.143 2.002 2.073 1.973    
    25 1.298 1.277 1.288 1.188    
    12.5 0.732 0.705 0.719 0.619    
    6.25 0.414 0.393 0.404 0.304    
    3.12 0.263 0.252 0.258 0.158    
    1.56 0.172 0.168 0.170 0.070    
    0 0.101 0.099 0.100 　    
  • 数据处理：
  • 货期：
    3-5 working days

产品评价

问答及客户评论

■ 常见问题解答
Q:

Are there any recombinant proteins in this kit?
If so, what are the host cells?
Are there any components containing azide, thimerosal or 2-ME?
What is the composition of the Stop solution?

A:
Thanks for your inquiry!
Pls check our information:
Standard: CHO cell-derived recombinant protein
There is no component containing azide, thimerosal or 2-ME with this kit.
Stop solution: 4N sulfuric acid

靶点详情

  • 功能：
    Promotes the growth of epithelial cells. May stimulate the phosphorylation of EGFR and mitogen-activated protein kinases.
  • 基因功能参考文献：
    1. Study shows how the EGFR ligands epiregulin (EREG) and epigen (EPGN) stabilize different dimeric conformations of the EGFR extracellular region. Results reveal how responses to different EGFR ligands are defined by receptor dimerization strength and signaling dynamics. These findings have broad implications for understanding receptor tyrosine kinase (RTK) signaling specificity. PMID: 28988771
    2. cloning of a related gene in mouse PMID: 112783
    3. EPG is an EGF-like growth factor and a low affinity ligand PMID: 15611079
  • 亚细胞定位：
    [Isoform 1]: Membrane; Single-pass type I membrane protein.; [Isoform 2]: Membrane; Single-pass type I membrane protein.; [Isoform 3]: Secreted.; [Isoform 4]: Secreted.; [Isoform 5]: Secreted.; [Isoform 6]: Secreted.
  • 数据库链接：

    HGNC: 17470

    KEGG: hsa:255324

    UniGene: Hs.401237