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  4. Human Folic acid(FA) ELISA Kit

Human Folic acid(FA) ELISA Kit

  • 中文名称：
    人叶酸(FA)酶联免疫试剂盒
  • 货号：
    CSB-E17109h
  • 规格：
    96T/48T
  • 价格：
    ￥3600/￥2500
  • 其他：

产品详情

  • 产品描述：
        叶酸化学名为N-（4-羧基-2-氧代-1，2，3，4-四氢喹啉-6-基）-L-谷氨酸，是一种水溶性维生素。它在体内参与DNA和RNA的合成、维持神经系统的正常功能以及红细胞的形成。缺乏叶酸会导致巨幼红细胞贫血和神经系统疾病等健康问题。
        j9九游会登录入口首页生物所提供的Human Folic acid(FA) ELISA Kit属于ELISA检测试剂盒，采用竞争法定量检测人血清、血浆样本中的FA，其灵敏度为6.25 pg/mL，检测范围为12.5 pg/mL-200 pg/mL。
     
  • 别名：
    N/A
  • 缩写：
    FA
  • 种属：
    Homo sapiens (Human)
  • 样本类型：
    serum, plasma
  • 检测范围：
    12.5 pg/mL-200 pg/mL
  • 灵敏度：
    6.25 pg/mL
  • 反应时间：
    1-5h
  • 样本体积：
    50-100ul
  • 检测波长：
    450 nm
  • 研究领域：
    Metabolism
  • 测定原理：
    quantitative
  • 测定方法：
    Competitive
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%      
    Three samples of known concentration were tested twenty times on one plate to assess.  
    Inter-assay Precision (Precision between assays): CV%<10%      
    Three samples of known concentration were tested in twenty assays to assess.    
                 
  • 线性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of human FA in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    　 Sample Serum(n=4)  
    1:20 Average % 89  
    Range % 84-93  
    1:40 Average % 93  
    Range % 89-97  
    1:80 Average % 95  
    Range % 90-100  
    1:160 Average % 84  
    Range % 80-88  
  • 回收率：
    The recovery of human FA spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample Type Average % Recovery Range  
    Serum (n=5) 99 94-105  
    EDTA plasma (n=4) 95 90-99  
                 
                 
  • 标准曲线：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    pg/ml OD1 OD2 Average    
    200 0.093 0.090 0.092    
    100 0.227 0.208 0.218    
    50 0.378 0.367 0.373    
    25 0.618 0.637 0.628    
    12.5 0.986 1.003 0.995    
    0 1.485 1.429 1.457    
  • 本试剂盒所含材料：
      • A 96-well Assay plate --The 96-well plate has been pre-coated with anti-human FA antibody.
      • Standard (10 x concentrate)(1 x 200 μl) --Dilute the standard at dilution series, read the OD values, and then draw a standard curve.
      • HRP-conjugated FA (100 x concentrate) (1 x 6oμl)
      • HRP-conjugate Diluent (1 x 10 ml) --Dilute the HRP-conjugated FA antibody solution.
      • Sample Diluent (2 x 20 ml) --Reconstitute the standard and dilute the sample to an appropriate concentration.
      • Wash Buffer (25x concentrate) (1 x 20 ml)--Wash away unbound or free substances.
      • TMB Substrate (1x 10 ml) --Act as the chromogenic agent. TMB interacts with HRP, eliciting the solution turns blue.
      • Stop Solution (1 x 10ml) --Stop the color reaction. The solution color immediately turns from blue to yellow.
      • Four Adhesive Strips (For 96 wells)
      • An Instruction manual

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  • 本试剂盒不含材料：
      • A microplate reader capable of measuring absorbance at 450 nm, with the correction wavelength set at 540 nm - 570 nm.
      • An incubator that can provide stable incubation conditions up to 37°C±5°C.
      • Centrifuge
      • Vortex
      • Squirt bottle, manifold dispenser, or automated microplate washer
      • Absorbent paper for blotting the microtiter plate
      • 50-300ul multi-channel micropipette
      • Pipette tips
      • Single-channel micropipette with different ranges
      • 100ml and 500ml graduated cylinders
      • Deionized or distilled water
      • Timer
      • Test tubes for dilution

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  • 数据处理：
  • 货期：
    3-5 working days

产品评价

问答及客户评论

■ 常见问题解答
Q:

We are looking for Elisa kit for human folic acid, detection range: 310-1000 nmol/L.
Material: erythrocytes (not full blood)
Please tell me is the result obtained from your kit can be comparable to the microbiological reference method?

A:
Thank you for your enquiry!Currently we just have this kit, and the detailed information, pls check the manual.
The theory of different detection methods are different.Since we have no information which is comparable to the microbiological reference method.
Pls just take your own actual test result.

靶点详情

  • 最新研究进展：
        叶酸（Folic acid）是一种维生素B族成员，参与人体DNA、RNA的合成和修复，对于细胞生长和分裂非常重要。最新的研究表明，叶酸在人体内具有多种生理功能，包括对于心血管健康和神经系统健康的影响，以及对于出生缺陷和某些类型的癌症的预防作用。
        一项对临床试验的回顾性分析发现，叶酸可以降低缺血性心血管疾病（如冠心病和脑卒中）的发生率和死亡率。另一项研究显示，叶酸可以促进神经细胞的发育和修复，有助于预防老年痴呆症和帕金森病等神经系统疾病的发生。
    此外，叶酸还可以预防某些类型的出生缺陷，如脊柱裂和唇裂等，因为这些缺陷通常与母体叶酸不足有关。对于某些类型的癌症，如结肠癌和乳腺癌等，一些研究表明，适当的叶酸摄入可以减少其发生的风险。