Your Good Partner in Biology Research

  1. 首页
  2. 试剂盒
  3. 酶联免疫试剂盒
  4. Human Galanin-like peptide(GALP) ELISA kit

Human Galanin-like peptide(GALP) ELISA kit

  • 中文名称：
    人甘丙肽样肽(GALP)酶联免疫试剂盒
  • 货号：
    CSB-EL009222HU
  • 规格：
    96T/48T
  • 价格：
    ￥3600/￥2500
  • 其他：

产品详情

  • 产品描述：

    This Human GALP ELISA Kit was designed for the quantitative measurement of Human GALP protein in serum, plasma, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 0.156 ng/mL-10 ng/mL and the sensitivity is 0.039 ng/mL.

  • 别名：
    GALP ELISA Kit; Galanin-like peptide ELISA Kit
  • 缩写：
    GALP
  • Uniprot No.：
  • 种属：
    Homo sapiens (Human)
  • 样本类型：
    serum, plasma, tissue homogenates
  • 检测范围：
    0.156 ng/mL-10 ng/mL
  • 灵敏度：
    0.039 ng/mL
  • 反应时间：
    1-5h
  • 样本体积：
    50-100ul
  • 检测波长：
    450 nm
  • 研究领域：
    Cardiovascular
  • 测定原理：
    quantitative
  • 测定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%
    Three samples of known concentration were tested in twenty assays to assess.
  • 线性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of human GALP in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    SampleSerum(n=4)
    1:1Average %93
    Range %87-97
    1:2Average %101
    Range %97-103
    1:4Average %95
    Range %88-98
    1:8Average %99
    Range %94-102
  • 回收率：
    The recovery of human GALP spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample TypeAverage % RecoveryRange
    Serum (n=5) 9892-102
    EDTA plasma (n=4)9488-97
  • 标准曲线：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    ng/mlOD1OD2AverageCorrected
    102.057 2.080 2.069 1.918
    51.761 1.812 1.787 1.636
    2.51.403 1.373 1.388 1.237
    1.250.988 0.973 0.981 0.830
    0.6250.703 0.727 0.715 0.564
    0.3120.477 0.497 0.487 0.336
    0.1560.322 0.331 0.327 0.176
    00.150 0.152 0.151
  • 数据处理：
  • 货期：
    3-5 working days

产品评价

靶点详情

  • 功能：
    Hypothalamic neuropeptide which binds to the G-protein-coupled galanin receptors (GALR1, GALR2 and GALR3). Involved in a large number of putative physiological functions in CNS homeostatic processes, including the regulation of gonadotropin-releasing hormone secretion.; Exhibits potent and dose-dependent vasoconstrictor and anti-edema activity in the cutaneous microvasculature, a physiologic effects which does not appear to be mediated via GALR1 or GALR2. Exhibits antimicrobial activity against Gram-negative bacterias, inducing bacterial membrane blebbing.
  • 基因功能参考文献：
    1. results indicated that obese individuals have higher plasma GAL and GALP concentrations and both peptide concentrations were positively correlative to triglyceride concentrations in obese human PMID: 27474259
    2. One isoform (alarin) of this gene but not the other (galanin-like peptide) displays antimicrobial activity against E. coli. PMID: 23537644
    3. This review presents considerable evidence that has accumulated implicating both galanin and GALP as playing important roles in regulating food and water intake behavior and related neuroendocrine functions. PMID: 16787232
    4. describes cloning of the full-length human GALP PMID: 10601261
    5. describes the gene structure of human GALP. PMID: 11861493
    6. reports immunostaining of human GALP in the dermal microvasculature. PMID: 17535903

    显示更多

    收起更多

  • 亚细胞定位：
    Secreted.
  • 蛋白家族：
    Galanin family
  • 组织特异性：
    Isoform 2 is found in ganglia of ganglioneuroma and ganglioneuroblastoma, as well as in differentiated tumor cells of neuroblastoma tissues. Not found in undifferentiated neuroblasts. Isoform 2 is found in the skin, in pericytes covering microvascular art
  • 数据库链接：

    HGNC: 24840

    OMIM: 611178

    KEGG: hsa:85569

    STRING: 9606.ENSP00000349884

    UniGene: Hs.283915