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中文名称:人神经节苷脂GM2活化剂(GM2A)酶联免疫试剂盒
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货号:CSB-EL009565HU
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规格:96T/48T
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价格:¥3600/¥2500
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其他:
产品详情
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产品描述:
This Human GM2A ELISA Kit was designed for the quantitative measurement of Human GM2A protein in serum, plasma, urine, tissue homogenates, cell lysates. It is a Sandwich ELISA kit, its detection range is 15.6 pg/mL-1000 pg/mL and the sensitivity is 3.9 pg/mL.
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别名:Cerebroside sulfate activator protein ELISA Kit; ganglioside GM2 activator ELISA Kit; Ganglioside GM2 activator isoform short ELISA Kit; Ganglioside GM2 activator precursor ELISA Kit; GM2 activator ELISA Kit; GM2 AP ELISA Kit; GM2 ganglioside activator ELISA Kit; GM2 ganglioside activator protein ELISA Kit; GM2-AP ELISA Kit; GM2A ELISA Kit; GM2AP ELISA Kit; OTTHUMP00000160619 ELISA Kit; SAP 3 ELISA Kit; SAP-3 ELISA Kit; SAP3 ELISA Kit; SAP3_HUMAN ELISA Kit; Shingolipid activator protein 3 ELISA Kit; Sphingolipid activator protein 3 ELISA Kit
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缩写:GM2A
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Uniprot No.:
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种属:Homo sapiens (Human)
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样本类型:serum, plasma, urine, tissue homogenates, cell lysates
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检测范围:15.6 pg/mL-1000 pg/mL
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灵敏度:3.9 pg/mL
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反应时间:1-5h
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样本体积:50-100ul
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检测波长:450 nm
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研究领域:Metabolism
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测定原理:quantitative
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测定方法:Sandwich
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精密度:
Intra-assay Precision (Precision within an assay): CV%<8% Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision (Precision between assays): CV%<10% Three samples of known concentration were tested in twenty assays to assess. -
线性度:
To assess the linearity of the assay, samples were spiked with high concentrations of human GM2A in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. Sample Serum(n=4) 1:1 Average % 89 Range % 84-95 1:2 Average % 92 Range % 87-99 1:4 Average % 100 Range % 95-106 1:8 Average % 97 Range % 92-102 -
回收率:
The recovery of human GM2A spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. Sample Type Average % Recovery Range Serum (n=5) 96 90-103 EDTA plasma (n=4) 90 84-96 -
标准曲线:
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. pg/ml OD1 OD2 Average Corrected 1000 2.043 2.050 2.047 1.921 500 1.619 1.684 1.652 1.526 250 1.105 1.200 1.153 1.027 125 0.683 0.703 0.693 0.567 62.5 0.403 0.421 0.412 0.286 31.2 0.296 0.322 0.309 0.183 15.6 0.241 0.235 0.238 0.112 0 0.123 0.129 0.126 -
数据处理:
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货期:3-5 working days
引用文献
相关产品
靶点详情
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功能:The large binding pocket can accommodate several single chain phospholipids and fatty acids, GM2A also exhibits some calcium-independent phospholipase activity. Binds gangliosides and stimulates ganglioside GM2 degradation. It stimulates only the breakdown of ganglioside GM2 and glycolipid GA2 by beta-hexosaminidase A. It extracts single GM2 molecules from membranes and presents them in soluble form to beta-hexosaminidase A for cleavage of N-acetyl-D-galactosamine and conversion to GM3. Has cholesterol transfer activity.
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基因功能参考文献:
- Review of GM2A mutations causing GM2 activator protein deficiency and GM2 gangliosidosis-AB variant. PMID: 27402091
- Studies indicate that sphingolipid activator proteins (SAPs) and anionic lipids are essential stimulators to reach physiological rates of lysosomal sphingolipid degradation. PMID: 27157270
- this study has established the potential role of GM2A in breast cancer progression PMID: 27002480
- Mobilization of membrane lipids by GM2AP was also inhibited in the presence of cholesterol or SM, as revealed by surface plasmon resonance studies PMID: 26175473
- Gene polymorphisms of MD2 and GM2A were associated with the occurrence or severity of neonatal necrotizing enterocolitis. PMID: 25816011
- In vitro assays with the isolated H1 or H2 homodimers (beta-alpha hybrid construct of beta-hexosaminidase A subunits) confirmed that neither was capable of human GM2AP-dependent hydrolysis of GM2 ganglioside. PMID: 23483939
- impact of GM2AP on glucose metabolism PMID: 21784073
- Treatment of meniscal explants with IL-1RA inhibited the expression of many catabolic genes following a single bout of high dynamic strain. PMID: 21331553
- these results provide novel insights into the physiological functions of GM2AP in obesity. PMID: 21036149
- GM2 activator protein exerts strong and broad inhibitory effects on the hydrolysis of phospholipids carried out by plant and microbial phospholipases D PMID: 12576516
- Two new structures of GM2-AP with bound lipids, showing two different lipid-binding modes within the apolar pocket PMID: 12909021
- elucidation of mode of action on gangioside GM2 PMID: 14728689
- alpha-subunit loop structure is required for GM2 activator protein binding by beta-hexosaminidase A PMID: 15485660
- glycosphingolipids, particularly GM2, form a complex with CD82, and this complex interacts with Met and thereby inhibits HGF-induced Met tyrosine kinase activity, as well as integrin to Met cross-talk PMID: 17215249
收起更多
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相关疾病:GM2-gangliosidosis AB (GM2GAB)
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亚细胞定位:Lysosome.
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数据库链接: