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  4. Human Hyaluronic acid,HA ELISA Kit

Human Hyaluronic acid,HA ELISA Kit

  • 中文名称：
    人透明质酸(HA)酶联免疫试剂盒
  • 货号：
    CSB-E04805h
  • 规格：
    96T/48T
  • 价格：
    ￥3600/￥2500
  • 其他：

产品详情

  • 产品描述：
        HA（透明质酸）是一种广泛存在于哺乳动物组织中的生物高分子，具有保湿、润滑、营养、细胞外基质支撑、生长和迁移调节等多种生物学功能。在人体内，HA的含量与一些疾病如关节炎、皮肤老化、干眼症、糖尿病等有关
        j9九游会登录入口首页生物所提供的Human Hyaluronic acid,HA ELISA Kit属于ELISA检测试剂盒，采用双抗夹心法定量检测人血清、血浆、脑脊液(CSF)、组织匀浆样本中的HA，其灵敏度为0.039 ng/mL，检测范围为0.156 ng/mL-10 ng/mL。
     
  • 别名：
    N/A
  • 缩写：
    HA
  • 种属：
    Homo sapiens (Human)
  • 样本类型：
    serum, plasma, cerebrospinal fluid (CSF), tissue homogenates
  • 检测范围：
    0.156 ng/mL-10 ng/mL
  • 灵敏度：
    0.039 ng/mL
  • 反应时间：
    1-5h
  • 样本体积：
    50-100ul
  • 检测波长：
    450 nm
  • 研究领域：
    Cell Biology
  • 测定原理：
    quantitative
  • 测定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%      
    Three samples of known concentration were tested twenty times on one plate to assess.  
    Inter-assay Precision (Precision between assays): CV%<10%      
    Three samples of known concentration were tested in twenty assays to assess.    
                 
  • 线性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of human HA in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    　 Sample Serum(n=4)  
    1:20 Average % 87  
    Range % 80-94  
    1:40 Average % 92  
    Range % 88-99  
    1:80 Average % 97  
    Range % 91-100  
    1:160 Average % 101  
    Range % 97-105  
  • 回收率：
    The recovery of human HA spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample Type Average % Recovery Range  
    Serum (n=5) 100 95-105  
    EDTA plasma (n=4) 93 88-98  
                 
                 
  • 标准曲线：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    ng/ml OD1 OD2 Average Corrected  
    10 2.727 2.700 2.714 2.626  
    5 2.231 2.345 2.288 2.200  
    2.5 1.558 1.655 1.607 1.519  
    1.25 0.831 0.901 0.866 0.778  
    0.625 0.477 0.451 0.464 0.376  
    0.312 0.301 0.322 0.312 0.224  
    0.156 0.189 0.174 0.182 0.094  
    0 0.087 0.089 0.088 　  
  • 本试剂盒所含材料：
      • A micro ELISA plate --- The 96-well plate has been pre-coated with an anti-human HA antibody. This dismountable microplate can be divided into 12 x 8 strip plates.
      • Two vials lyophilized standard --- Dilute a bottle of the standard at dilution series, read the OD values, and then draw a standard curve.
      • Biotin-labeled HA antibody(100 x concentrate) 1 x 120 μl ---Act as the detection antibody.
      • HRP-avidin (100 x concentrate) 1 x 120 μl --- Bind to the detection antibody and react with the TMB substrate to make the solution chromogenic.
      • Biotin-antibody Diluent 1 x 15 ml ---Dilute the high concentration Biotin-antibody to an appropriate working solution.
      • HRP-avidin Diluent 1 x 15 ml ---Dilute the high concentration HRP-avidin solution to an appropriate solution.
      • Sample Diluent 1 x 50 ml---Dilute the sample to an appropriate concentration.
      • Wash Buffer (25 x concentrate) 1 x 20 ml --- Wash away unbound or free substances.
      • TMB Substrate 1 x 10 ml --- Act as the chromogenic agent. TMB interacts with HRP, eliciting the solution turns blue.
      • Stop Solution 1 x 10 ml --- Stop the color reaction. The solution color immediately turns from blue to yellow.
      • Four Adhesive Strips (For 96 wells) --- Cover the microplate when incubation.
      • An instruction manual

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  • 本试剂盒不含材料：
      • A microplate reader capable of measuring absorbance at 450 nm, with the correction wavelength set at 540 nm or 570 nm.
      • An incubator that can provide stable incubation conditions up to 37°C±5°C.
      • Centrifuge
      • Vortex
      • Squirt bottle, manifold dispenser, or automated microplate washer
      • Absorbent paper for blotting the microtiter plate
      • 50-300ul multi-channel micropipette
      • Pipette tips
      • Single-channel micropipette with different ranges
      • 100ml and 500ml graduated cylinders
      • Deionized or distilled water
      • Timer
      • Test tubes for dilution

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  • 数据处理：
  • 货期：
    3-5 working days

引用文献

产品评价

平均分:
5.0分 - 1 个评价

样品类型：细胞培养上清液

样品信息：人

稀释比：1:100

产品评价： 我用CSB-E04805h检测血清样本中HA含量，试剂盒灵敏度高，数据理想。

By 张老师

问答及客户评论

■ 常见问题解答
Q:

We are looking into the best method to distinguish HA manufactured by fermentation and nature human HA.

A:
Thanks for your inquiry.
CSB-E04805h Human Hyaluronic acid, HA ELISA Kit
This kit can detect nature HA in polymerized form. As for HA manufactured by fermentation, it usually exists in free form.
We think it can not be detected out by the kit.

靶点详情

  • 最新研究进展：
    HA（透明质酸）是一种大分子多糖，广泛存在于人体组织中。最近的研究表明，HA在细胞间通讯、细胞迁移和组织再生中发挥重要作用。HA在软骨组织再生和修复中的应用也受到广泛关注。研究人员正在开发一些基于HA的药物和材料，用于治疗各种软骨损伤和疾病，如骨关节炎、肩周炎和韧带损伤等。