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  4. Human Ig gamma-3 chain C region(IGHG3) ELISA kit

Human Ig gamma-3 chain C region(IGHG3) ELISA kit

  • 中文名称：
    人Igγ3链C区(IGHG3)酶联免疫试剂盒
  • 货号：
    CSB-EL011158HU
  • 规格：
    96T/48T
  • 价格：
    ￥3600/￥2500
  • 其他：

产品详情

  • 产品描述：

    This Human IGHG3 ELISA Kit was designed for the quantitative measurement of Human IGHG3 protein in serum, plasma. It is a Sandwich ELISA kit, its detection range is 2.7 ng/mL-2000 ng/mL and the sensitivity is 2.7 ng/mL .

  • 别名：
    IGHG3Immunoglobulin heavy constant gamma 3 ELISA Kit; HDC ELISA Kit; Heavy chain disease protein ELISA Kit; Ig gamma-3 chain C region ELISA Kit
  • 缩写：
    IGHG3
  • Uniprot No.：
  • 种属：
    Homo sapiens (Human)
  • 样本类型：
    serum, plasma
  • 检测范围：
    2.7 ng/mL-2000 ng/mL
  • 灵敏度：
    2.7 ng/mL
  • 反应时间：
    1-5h
  • 样本体积：
    50-100ul
  • 检测波长：
    450 nm
  • 研究领域：
    Immunology
  • 测定原理：
    quantitative
  • 测定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%
    Three samples of known concentration were tested in twenty assays to assess.
  • 线性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of human IGHG3 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    　SampleSerum(n=4)
    1:1000Average %99
    Range %93-106
    1:2000Average %90
    Range %85-95
    1:4000Average %87
    Range %82-91
    1:8000Average %93
    Range %86-98
  • 回收率：
    The recovery of human IGHG3 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample TypeAverage % RecoveryRange
    Serum (n=5) 9086-94
    EDTA plasma (n=4)9590-99
  • 标准曲线：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    ng/mlOD1OD2Average
    20000.173 0.172 0.1725
    6670.259 0.246 0.2525
    2220.367 0.344 0.3555
    740.488 0.459 0.4735
    250.633 0.624 0.6285
    8.20.789 0.855 0.822
    2.71.029 1.033 1.031
    01.697 1.748 1.7225
  • 数据处理：
  • 货期：
    3-5 working days

产品评价

靶点详情

  • 功能：
    Constant region of immunoglobulin heavy chains. Immunoglobulins, also known as antibodies, are membrane-bound or secreted glycoproteins produced by B lymphocytes. In the recognition phase of humoral immunity, the membrane-bound immunoglobulins serve as receptors which, upon binding of a specific antigen, trigger the clonal expansion and differentiation of B lymphocytes into immunoglobulins-secreting plasma cells. Secreted immunoglobulins mediate the effector phase of humoral immunity, which results in the elimination of bound antigens. The antigen binding site is formed by the variable domain of one heavy chain, together with that of its associated light chain. Thus, each immunoglobulin has two antigen binding sites with remarkable affinity for a particular antigen. The variable domains are assembled by a process called V-(D)-J rearrangement and can then be subjected to somatic hypermutations which, after exposure to antigen and selection, allow affinity maturation for a particular antigen.
  • 亚细胞定位：
    Secreted. Cell membrane.
  • 数据库链接：

    HGNC: 5527

    OMIM: 147120

    UniGene: Hs.510635