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  4. Human Immunoglobulin G4 (IgG4) ELISA Kit

Human Immunoglobulin G4 (IgG4) ELISA Kit

  • 中文名称：
    人免疫球蛋白G4(IgG4)酶联免疫试剂盒
  • 货号：
    CSB-E13728h
  • 规格：
    96T/48T
  • 价格：
    ￥3600/￥2500
  • 其他：

产品详情

  • 产品描述：

    This Human IgG4 ELISA Kit was designed for the quantitative measurement of Human IgG4 protein in serum, plasma. It is a Competitive ELISA kit, its detection range is 39 ng/mL-10000 ng/mL and the sensitivity is 39 ng/mL.

  • 缩写：
    IgG4
  • 种属：
    Homo sapiens (Human)
  • 样本类型：
    serum, plasma
  • 检测范围：
    39 ng/mL-10000 ng/mL
  • 灵敏度：
    39 ng/mL
  • 反应时间：
    1-5h
  • 样本体积：
    50-100ul
  • 检测波长：
    450 nm
  • 研究领域：
    Immunology
  • 测定原理：
    quantitative
  • 测定方法：
    Competitive
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%      
    Three samples of known concentration were tested twenty times on one plate to assess.  
    Inter-assay Precision (Precision between assays): CV%<10%      
    Three samples of known concentration were tested in twenty assays to assess.    
                 
  • 线性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of human IgG4 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    　 Sample Serum(n=4)  
    1:1000 Average % 96  
    Range % 91-101  
    1:2000 Average % 99  
    Range % 93-104  
    1:4000 Average % 92  
    Range % 87-96  
    1:8000 Average % 90  
    Range % 85-94  
  • 回收率：
    The recovery of human IgG4 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample Type Average % Recovery Range  
    Serum (n=5) 84 80-88  
    EDTA plasma (n=4) 93 88-97  
                 
                 
  • 标准曲线：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    ng/ml OD1 OD2 Average    
    10000 0.214 0.216 0.215    
    2500 0.598 0.601 0.600    
    625 0.998 1.013 1.006    
    156.25 1.597 1.614 1.606    
    39 1.869 1.889 1.879    
    0 2.344 2.368 2.356    
  • 数据处理：
  • 货期：
    3-5 working days

产品评价

问答及客户评论

■ 常见问题解答
Q:

How much reactions this kit included?

A:
Thanks for your inquiry. Regarding this kit,if you set single wells for standard,you can test 90 samples.If you duplicate the wells of standard,then you can test 45 samples.Pls let me know if you have any further questions. Thank you.