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  4. Human Immunoglobulin J chain(IGJ) ELISA kit

Human Immunoglobulin J chain(IGJ) ELISA kit

  • 中文名称：
    人免疫球蛋白j链(Ig-j)酶联免疫试剂盒
  • 货号：
    CSB-E09237h
  • 规格：
    96T/48T
  • 价格：
    ￥3600/￥2500
  • 其他：

产品详情

  • 产品描述：

    This Human IGJ ELISA Kit was designed for the quantitative measurement of Human IGJ protein in serum, plasma, saliva, urine, tear, breast milk. It is a Sandwich ELISA kit, its detection range is 10 ng/mL-40000 ng/mL and the sensitivity is 10 ng/mL.

  • 别名：
    IGCJ ELISA Kit; IGJ_HUMAN ELISA Kit; Immunoglobulin J chain ELISA Kit; Immunoglobulin J polypeptide linker protein for immunoglobulin alpha and mu polypeptides ELISA Kit; immunoglobulin joining chain ELISA Kit; JCH ELISA Kit; JCHAIN ELISA Kit; Joining chain of multimeric IgA and IgM ELISA Kit
  • 缩写：
    IGJ
  • Uniprot No.：
  • 种属：
    Homo sapiens (Human)
  • 样本类型：
    serum, plasma, saliva, urine, tear, breast milk
  • 检测范围：
    10 ng/mL-40000 ng/mL
  • 灵敏度：
    10 ng/mL
  • 反应时间：
    1-5h
  • 样本体积：
    50-100ul
  • 检测波长：
    450 nm
  • 研究领域：
    Immunology
  • 测定原理：
    quantitative
  • 测定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%        
    Three samples of known concentration were tested twenty times on one plate to assess.    
    Inter-assay Precision (Precision between assays):CV%<10%        
    Three samples of known concentration were tested in twenty assays to assess.      
                   
  • 线性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of human IGJ in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.  
    　 Sample Serum(n=4)    
    1:1000 Average % 89    
    Range % 81-99    
    1:2000 Average % 95    
    Range % 83-99    
    1:4000 Average % 95    
    Range % 90-102    
    1:8000 Average % 90    
    Range % 85-100    
  • 回收率：
    The recovery of human IGJ spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.  
     
    Sample Type Average % Recovery Range    
    Serum (n=5) 92 85-99    
    EDTA plasma (n=4) 95 90-100    
                   
                   
  • 标准曲线：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.  
     
    ng/ml OD1 OD2 Average Corrected    
    40000 2.257 2.242 2.250 2.162    
    10000 2.154 2.138 2.146 2.058    
    2500 1.892 1.864 1.878 1.790    
    625 1.252 1.249 1.251 1.163    
    156 0.587 0.598 0.593 0.505    
    39 0.282 0.288 0.285 0.197    
    10 0.141 0.139 0.140 0.052    
    0 0.086 0.090 0.088 　    
  • 数据处理：
  • 货期：
    3-5 working days

产品评价

靶点详情

  • 功能：
    Serves to link two monomer units of either IgM or IgA. In the case of IgM, the J chain-joined dimer is a nucleating unit for the IgM pentamer, and in the case of IgA it induces dimers and/or larger polymers. It also helps to bind these immunoglobulins to secretory component.
  • 基因功能参考文献：
    1. TLR2 ligands induce CCR9 and CCR10 expression by circulating B-cells and increase their chemotaxis. TLR2 stimulation also induced J chain and IgA production demonstrating the induction of mucosal-like antibody secreting cells. PMID: 20947433
  • 亚细胞定位：
    Secreted.
  • 数据库链接：

    HGNC: 5713

    OMIM: 147790

    KEGG: hsa:3512

    STRING: 9606.ENSP00000254801

    UniGene: Hs.643431