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Human Interferon kappa(IFNK) ELISA kit

  • 中文名称：
    人干扰素κ(IFNK)酶联免疫试剂盒
  • 货号：
    CSB-EL011053HU
  • 规格：
    96T/48T
  • 价格：
    ￥3600/￥2500
  • 其他：

产品详情

  • 产品描述：

    This Human IFNK ELISA Kit was designed for the quantitative measurement of Human IFNK protein in serum, plasma, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 15.6 pg/mL-1000 pg/mL and the sensitivity is 3.9 pg/mL.

  • 别名：
    IFN kappa ELISA Kit; IFN-kappa ELISA Kit; Ifnk ELISA Kit; IFNK_HUMAN ELISA Kit; Interferon kappa ELISA Kit
  • 缩写：
  • Uniprot No.：
  • 种属：
    Homo sapiens (Human)
  • 样本类型：
    serum, plasma, tissue homogenates
  • 检测范围：
    15.6 pg/mL-1000 pg/mL
  • 灵敏度：
    3.9 pg/mL
  • 反应时间：
    1-5h
  • 样本体积：
    50-100ul
  • 检测波长：
    450 nm
  • 研究领域：
    Immunology
  • 测定原理：
    quantitative
  • 测定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%        
    Three samples of known concentration were tested twenty times on one plate to assess.    
    Inter-assay Precision (Precision between assays): CV%<10%        
    Three samples of known concentration were tested in twenty assays to assess.      
                   
  • 线性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of human IFNK in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.  
    　 Sample Serum(n=4)    
    1:1 Average % 93    
    Range % 89-97    
    1:2 Average % 85    
    Range % 80-90    
    1:4 Average % 90    
    Range % 85-94    
    1:8 Average % 98    
    Range % 94-103    
  • 回收率：
    The recovery of human IFNK spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.  
     
    Sample Type Average % Recovery Range    
    Serum (n=5) 101 97-105    
    EDTA plasma (n=4) 92 88-96    
                   
                   
  • 标准曲线：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.  
     
    pg/ml OD1 OD2 Average Corrected    
    1000 2.694 2.758 2.726 2.622    
    500 2.309 2.182 2.246 2.142    
    250 1.515 1.601 1.558 1.454    
    125 0.954 1.004 0.979 0.875    
    62.5 0.521 0.560 0.541 0.437    
    31.25 0.287 0.293 0.290 0.186    
    15.6 0.193 0.205 0.199 0.095    
    0 0.100 0.107 0.104 　    
  • 数据处理：
  • 货期：
    3-5 working days

产品评价

靶点详情

  • 功能：
    May play a role in the regulation of immune cell function. Cytokine that imparts cellular protection against viral infection in a species-specific manner. Activates the interferon-stimulated response element signaling pathway. It is able to directly modulate cytokine release from monocytes and dendritic cells. Binds heparin.
  • 基因功能参考文献：
    1. investigation of common variable immunodeficiency (CVID) from 2 German families; report on the occurrence of a common and one novel truncating IFNK mutation in cases with CVID; the frequency distribution of c.30_31insTGTT in cases and controls as well as the observed segregation patterns in CVID families exclude IFNK mutations as major risk factor in CVID PMID: 28324805
    2. These results suggest that high-risk human papillomavirus 31 target interferon kappa to prevent Sp100 expression and identify Sp100 as an interferon-stimulated gene with anti-human papillomavirus activity. PMID: 26491169
    3. This study demonstrates that E2 proteins of high risk human papillomavirus reduce STING and IFN-kappa transcription. PMID: 24614210
    4. The viral E6 and E7 oncogenes are sufficient for interferon-kappa repression, with E6 being mainly responsible. PMID: 21849431
    5. IFN-kappa is down-regulated in cervical keratinocytes harboring HPV, which may be a contributing factor in the progression of a cervical lesion PMID: 20479716
    6. Studied IFNK single nucleotide polymorphisms in 3982 Systemic lupus erythematosus cases and 4275 controls. PMID: 20706608
    7. IFN-kappa is able to directly modulate cytokine release from monocytes and dendritic cells, inhibit inducible IL-12 release from monocytes, and bind strongly to heparin. PMID: 12391192
    8. This is the first report showing an epigenetic silencing of type I IFN after HPV16 oncogene expression PMID: 19887612

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  • 亚细胞定位：
    Secreted.
  • 蛋白家族：
    Alpha/beta interferon family
  • 组织特异性：
    Expressed in keratinocytes, monocytes and in resting dendritic cells.
  • 数据库链接：

    HGNC: 21714

    OMIM: 615326

    KEGG: hsa:56832

    STRING: 9606.ENSP00000276943

    UniGene: Hs.591083