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  4. Human Interleukin-36 alpha(IL1F6) ELISA kit

Human Interleukin-36 alpha(IL1F6) ELISA kit

  • 中文名称：
    人白介素36α(IL1F6)酶联免疫试剂盒
  • 货号：
    CSB-EL011617HU
  • 规格：
    96T/48T
  • 价格：
    ￥3600/￥2500
  • 其他：

产品详情

  • 产品描述：

    The human interleukin-36 alpha (IL36A) ELISA kit is suitable for quantitatively determining the human IL36A complex in multiple samples, including serum, plasma, tissue homogenates, or cell culture supernates. This assay employs the bi-antibody sandwich technique and enzyme-substrate chromogenic reaction to quantify human IL36A levels in the sample. The amount of synthesized colored product is positively related to the analyte of interest in the sample.

    IL36A, also called IL1F6, is expressed during embryonic development and is highly abundant in epithelial cells, as well as monocytes, B cells, and T cells. IL36A has been shown to exert strong pro-inflammatory effects in the lungs of mice. Along with its modulation by EGF, IL36A plays an important role in skin barrier function and homeostasis. Ichii et al. indicated an association between intrarenal IL36A overexpression and the progression of tubulointerstitial inflammations and morpho-functional changes of distal tubule (DT) epithelial cells. IL36A may be a novel kidney injury marker useful for evaluating DT damages.

  • 别名：
    FIL1 ELISA Kit; FIL1 epsilon ELISA Kit; FIL1(EPSILON) ELISA Kit; FIL1E ELISA Kit; IL 1 epsilon ELISA Kit; IL 1F6 ELISA Kit; IL 1H1 ELISA Kit; IL-1 epsilon ELISA Kit; IL-1F6 ELISA Kit; IL1E ELISA Kit; IL1F6 ELISA Kit; IL1F6_HUMAN ELISA Kit; IL1RP2 ELISA Kit; IL36 alpha ELISA Kit; IL36A ELISA Kit; Interleukin 1 epsilon ELISA Kit; Interleukin 1 family member 6 (epsilon) ELISA Kit; Interleukin 1 family member 6 ELISA Kit; Interleukin 36 alpha ELISA Kit; Interleukin-1 epsilon ELISA Kit; Interleukin-1 family member 6 ELISA Kit; MGC129552 ELISA Kit; MGC129553 ELISA Kit; MGC151479 ELISA Kit; MGC151481 ELISA Kit; OTTMUSP00000012798 ELISA Kit; RP23-176J12.4 ELISA Kit
  • 缩写：
    IL1F6
  • Uniprot No.：
  • 种属：
    Homo sapiens (Human)
  • 样本类型：
    serum, plasma, tissue homogenates, cell culture supernates
  • 检测范围：
    15.6 pg/mL-1000 pg/mL
  • 灵敏度：
    3.9 pg/mL
  • 反应时间：
    1-5h
  • 样本体积：
    50-100ul
  • 检测波长：
    450 nm
  • 研究领域：
    Immunology
  • 测定原理：
    quantitative
  • 测定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%
    Three samples of known concentration were tested in twenty assays to assess.
  • 线性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of human IL1F6 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    　SampleSerum(n=4)
    1:1Average %99
    Range %94-103
    1:2Average %87
    Range %80-95
    1:4Average %95
    Range %91-99
    1:8Average %93
    Range %86-98
  • 回收率：
    The recovery of human IL1F6 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample TypeAverage % RecoveryRange
    Serum (n=5) 9286-98
    EDTA plasma (n=4)9792-102
  • 标准曲线：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    pg/mlOD1OD2AverageCorrected
    10001.966 1.957 1.962 1.861
    5001.432 1.501 1.467 1.366
    2500.950 0.920 0.935 0.834
    1250.680 0.689 0.685 0.584
    62.50.465 0.502 0.484 0.383
    31.20.275 0.287 0.281 0.180
    15.60.182 0.178 0.180 0.079
    00.098 0.103 0.101 　
  • 数据处理：
  • 货期：
    3-5 working days

产品评价

靶点详情

  • 功能：
    Cytokine that binds to and signals through the IL1RL2/IL-36R receptor which in turn activates NF-kappa-B and MAPK signaling pathways in target cells linked to a pro-inflammatory response. Part of the IL-36 signaling system that is thought to be present in epithelial barriers and to take part in local inflammatory response; similar to the IL-1 system with which it shares the coreceptor IL1RAP. Seems to be involved in skin inflammatory response by acting on keratinocytes, dendritic cells and indirectly on T-cells to drive tissue infiltration, cell maturation and cell proliferation. In cultured keratinocytes induces the expression of macrophage, T-cell, and neutrophil chemokines, such as CCL3, CCL4, CCL5, CCL2, CCL17, CCL22, CL20, CCL5, CCL2, CCL17, CCL22, CXCL8, CCL20 and CXCL1, and the production of proinflammatory cytokines such as TNF-alpha, IL-8 and IL-6. In cultured monocytes upregulates expression of IL-1A, IL-1B and IL-6. In myeloid dendritic cells involved in cell maturation by upregulating surface expression of CD83, CD86 and HLA-DR. In monocyte-derived dendritic cells facilitates dendritic cell maturation and drives T-cell proliferation. May play a role in proinflammatory effects in the lung.
  • 基因功能参考文献：
    1. serum IL-36alpha levels were higher in active systemic lupus erythematosus patients and correlated with disease activity and arthritis PMID: 29571080
    2. Data suggest that interleukin-36alpha (IL-36alpha) plays an important role in the pathophysiology of inflammation and fibrosis in the pancreas via an autocrine function. PMID: 28099250
    3. The data identify a novel role for IL-36 signaling in colonic inflammation and indicate that the IL-36R pathway may represent a novel target for therapeutic intervention. PMID: 26813344
    4. IL-36a was displayed to be able to suppress the growth of epithelial ovarian cancer cells in our setting, which is suggestive of its druggable potential in curing the epithelial ovarian cancer and that upregulation of IL-36a was found to be capable of inhibiting the growth of epithelial ovarian cancer cells. PMID: 28621240
    5. Increased Expression of Interleukin-36 is associated with Inflammatory Bowel Disease. PMID: 26752465
    6. IL-36alpha acts as a pro-inflammatory cytokine at cartilage level, by increasing the expression of markers of inflammation and cartilage catabolism. PMID: 26560022
    7. Study shows that plasma concentrations of IL-36alpha and IL-36gamma are overexpressed in active systemic lupus erythematosus patients and that IL-36alpha has a substantial pro-inflammatory effect through regulation of IL-6 and CXCL8 production. PMID: 26516833
    8. increases maturation of monocyte-derived dendritic cells PMID: 25700962
    9. IL36A-IL36R axis is modulated in patients with primary Sjogren's syndrome. PMID: 25902739
    10. IL-36alpha was highly expressed in nearly half of all colorectal cancer patients. Low expression level of IL-36alpha correlated with larger tumor size and advanced cancer stage. Low expression of IL-36alpha resulted in a poor prognosis of colorectal cancer patients. PMID: 25550854
    11. Results show that IL-36alpha expression may play a pivotal role in determining the prognosis of hepatocellular carcinoma (HCC). PMID: 24061617
    12. The novel cytokine interleukin-36alpha is expressed in psoriatic and rheumatoid arthritis synovium. PMID: 23268368
    13. Expression of IL-1F6 is increased in human plaque psoriasis skin and in the lesional skin of transgenic mice compared with monotransgenic littermates. PMID: 21242515
    14. A functional IL-6 polymorphism has been weakly associated with level of peak bone mineral density and the rate of forearm trabecular postmenopausal bone loss in a cohort of women. PMID: 12110411
    15. Dysregulated expression in transgenic mice can promote cutaneous inflammation, revealing potential novel targets for the treatment of inflammatory skin disorders. PMID: 17908936
    16. IL-1 epsilon, an interleukin-1 agonist, activates NF-kappa B through the orphan IL-1 receptor-related protein 2 and may constitute part of an independent signaling system present in human epithelial barriers. PMID: 11466363

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  • 亚细胞定位：
    Cytoplasm. Secreted.
  • 蛋白家族：
    IL-1 family
  • 组织特异性：
    Expressed in immune system and fetal brain, but not in other tissues tested or in multiple hematopoietic cell lines. Predominantly expressed in skin keratinocytes but not in fibroblasts, endothelial cells or melanocytes. Increased in lesional psoriasis sk
  • 数据库链接：

    HGNC: 15562

    OMIM: 605509

    KEGG: hsa:27179

    STRING: 9606.ENSP00000259211

    UniGene: Hs.278910