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  4. Human Leucine-rich repeat-containing protein 17(LRRC17) ELISA kit

Human Leucine-rich repeat-containing protein 17(LRRC17) ELISA kit

  • 中文名称：
    人富亮氨酸重复序列蛋白17(LRRC17)酶联免疫试剂盒
  • 货号：
    CSB-EL013112HU
  • 规格：
    96T/48T
  • 价格：
    ￥3600/￥2500
  • 其他：

产品详情

  • 产品描述：

    This Human LRRC17 ELISA Kit was designed for the quantitative measurement of Human LRRC17 protein in serum, plasma, tissue homogenates, cell lysates. It is a Sandwich ELISA kit, its detection range is 23.44 pg/mL-1500 pg/mL and the sensitivity is 5.86 pg/mL.

  • 别名：
    LRRC17 ELISA Kit; P37NB ELISA Kit; UNQ3076/PRO9909 ELISA Kit; Leucine-rich repeat-containing protein 17 ELISA Kit; p37NB ELISA Kit
  • 缩写：
    LRRC17
  • Uniprot No.：
  • 种属：
    Homo sapiens (Human)
  • 样本类型：
    serum, plasma, tissue homogenates, cell lysates
  • 检测范围：
    23.44 pg/mL-1500 pg/mL
  • 灵敏度：
    5.86 pg/mL
  • 反应时间：
    1-5h
  • 样本体积：
    50-100ul
  • 检测波长：
    450 nm
  • 研究领域：
    Signal Transduction
  • 测定原理：
    quantitative
  • 测定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%
    Three samples of known concentration were tested in twenty assays to assess.
  • 线性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of human LRRC17 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    　SampleSerum(n=4)
    1:1Average %95
    Range %90-101
    1:2Average %92
    Range %85-98
    1:4Average %86
    Range %80-92
    1:8Average %94
    Range %90-100
  • 回收率：
    The recovery of human LRRC17 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample TypeAverage % RecoveryRange
    Serum (n=5) 9287-99
    EDTA plasma (n=4)9591-101
  • 标准曲线：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    pg/mlOD1OD2AverageCorrected
    15001.711 1.672 1.692 1.562
    7501.426 1.396 1.411 1.281
    3751.012 1.004 1.008 0.878
    187.50.775 0.756 0.766 0.636
    93.750.583 0.544 0.564 0.434
    46.870.374 0.359 0.367 0.237
    23.440.262 0.247 0.255 0.125
    00.133 0.127 0.130 　
  • 数据处理：
  • 货期：
    3-5 working days

产品评价

靶点详情

  • 功能：
    Involved in bone homeostasis. Acts as a negative regulator of RANKL-induced osteoclast precursor differentiation from bone marrow precursors.
  • 基因功能参考文献：
    1. low circulating plasma level of LRRc17 might serve as an independent and additive risk factor for osteoporotic fractures. PMID: 27355564
    2. Whole-genome microarray mRNA-expression profiles of myofibroblasts and skin fibroblasts revealed four additional genes that are significantly differentially expressed in these two cell types: NKX2-3 and LRRC17 in myofibroblasts and SHOX2 and TBX5 in skin fibroblasts PMID: 27036009
  • 亚细胞定位：
    Secreted, extracellular space.
  • 组织特异性：
    Expressed in osteoblast cell lines. Well expressed in ovary, heart, pancreas, skeletal muscle, lung, and fetal kidney and lung and only at the basal levels in the other tissues examined including adult kidney. More expressed in S-type neuroblastoma cells
  • 数据库链接：

    HGNC: 16895

    KEGG: hsa:10234

    STRING: 9606.ENSP00000344242

    UniGene: Hs.567412