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  4. Human Matrix metalloproteinase 1,MMP-1 ELISA kit

Human Matrix metalloproteinase 1,MMP-1 ELISA kit

  • 中文名称：
    人基质金属蛋白酶1(MMP-1)酶联免疫试剂盒
  • 货号：
    CSB-E04672h
  • 规格：
    96T/48T
  • 价格：
    ￥3200/￥2500
  • 其他：

产品详情

  • 产品描述：
        MMP1（基质金属蛋白酶1，Matrix metalloproteinase-1），MMP1是一种由细胞外基质中的成纤维细胞、滑膜细胞、肌肉细胞、成骨细胞等分泌的酶类，属于基质金属蛋白酶家族，能够分解胶原蛋白和基质成分。MMP1在组织重塑、胚胎发育、创伤修复等生理过程中发挥着重要作用。但是，在某些疾病的发生和发展中，如炎症、纤维化、血管新生、肿瘤转移等，MMP1的异常表达可能会导致组织破坏和病情加重。因此，MMP1的测定可以作为某些疾病的辅助诊断指标之一，有一定的临床意义。
        j9九游会登录入口首页生物所提供的Human Matrix metalloproteinase 1,MMP-1 ELISA kit属于ELISA检测试剂盒，采用双抗夹心法定量检测人血清、血浆、细胞培养上清、组织匀浆样本中的MMP1，其灵敏度为0.078 ng/mL，检测范围为0.312 ng/mL-20 ng/mL。
     
  • 别名：
    27 kDa interstitial collagenase ELISA Kit; CLG ELISA Kit; CLGN ELISA Kit; collagenase; fibroblast ELISA Kit; collagenase; interstitial ELISA Kit; Fibroblast collagenase ELISA Kit; Interstitial collagenase ELISA Kit; Matrix metallopeptidase 1 (interstitial collagenase) ELISA Kit; Matrix metalloprotease 1 ELISA Kit; Matrix Metalloproteinase 1 ELISA Kit; Matrix metalloproteinase-1 ELISA Kit; MMP 1 ELISA Kit; MMP-1 ELISA Kit; MMP1 ELISA Kit; MMP1_HUMAN ELISA Kit; OTTHUMP00000045866 ELISA Kit
  • 缩写：
  • Uniprot No.：
  • 种属：
    Homo sapiens (Human)
  • 样本类型：
    serum, plasma, cell culture supernates, tissue homogenates
  • 检测范围：
    0.312 ng/mL-20 ng/mL
  • 灵敏度：
    0.078 ng/mL
  • 反应时间：
    1-5h
  • 样本体积：
    50-100ul
  • 检测波长：
    450 nm
  • 研究领域：
    Cancer
  • 测定原理：
    quantitative
  • 测定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%      
    Three samples of known concentration were tested twenty times on one plate to assess.  
    Inter-assay Precision (Precision between assays): CV%<10%      
    Three samples of known concentration were tested in twenty assays to assess.    
                 
  • 线性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of human MMP-1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    　 Sample Serum(n=4)  
    1:1 Average % 98  
    Range % 93-105  
    1:2 Average % 92  
    Range % 85-99  
    1:4 Average % 103  
    Range % 95-108  
    1:8 Average % 96  
    Range % 90-102  
  • 回收率：
    The recovery of human MMP-1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample Type Average % Recovery Range  
    Serum (n=5) 94 90-98  
    EDTA plasma (n=4) 96 90-100  
                 
                 
  • 标准曲线：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    ng/ml OD1 OD2 Average Corrected  
    20 2.244 2.404 2.324 2.122  
    10 1.556 1.587 1.572 1.370  
    5 1.062 1.081 1.072 0.870  
    2.5 0.709 0.736 0.723 0.521  
    1.25 0.476 0.499 0.488 0.286  
    0.625 0.374 0.397 0.386 0.184  
    0.312 0.330 0.336 0.333 0.131  
    0 0.201 0.203 0.202 　  
  • 本试剂盒所含材料：
      • A micro ELISA plate ---The 96-well plate has been pre-coated with an anti-human MMP-1 antibody. This dismountable microplate can be divided into 12 x 8 strip plates.
      • Two vials lyophilized standard ---Dilute a bottle of the standard at dilution series, read the OD values, and then draw a standard curve.
      • One vial Biotin-labeled MMP-1 antibody (100 x concentrate) (120 μl/bottle) ---Act as the detection antibody.
      • One vial HRP-avidin (100 x concentrate) (120 μl/bottle) ---Bind to the detection antibody and react with the TMB substrate to make the solution chromogenic.
      • One vial Biotin-antibody Diluent (15 ml/bottle) ---Dilute the Biotin-antibody.
      • One vial HRP-avidin Diluent (15 ml/bottle) ---Dilute the HRP-avidin solution.
      • One vial Sample Diluent (50 ml/bottle)---Dilute the sample to an appropriate concentration.
      • One vial Wash Buffer (25 x concentrate) (20 ml/bottle) ---Wash away unbound or free substances.
      • One vial TMB Substrate (10 ml/bottle) ---Act as the chromogenic agent. TMB interacts with HRP, eliciting the solution turns blue.
      • One vial Stop Solution (10 ml/bottle) ---Stop the color reaction. The solution color immediately turns from blue to yellow.
      • Four Adhesive Strips (For 96 wells) --- Cover the microplate when incubation.
      • An instruction manual

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  • 本试剂盒不含材料：
      • A microplate reader capable of measuring absorbance at 450 nm, with the correction wavelength set at 540 nm or 570 nm.
      • An incubator can provide stable incubation conditions up to 37°C±5°C.
      • Centrifuge
      • Vortex
      • Squirt bottle, manifold dispenser, or automated microplate washer
      • Absorbent paper for blotting the microtiter plate
      • 50-300ul multi-channel micropipette
      • Pipette tips
      • Single-channel micropipette with different ranges
      • 100ml and 500ml graduated cylinders
      • Deionized or distilled water
      • Timer
      • Test tubes for dilution

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  • 数据处理：
  • 货期：
    3-5 working days

引用文献

产品评价

靶点详情

  • 最新研究进展：
    MMP1是一种基质金属蛋白酶，全名为基质金属蛋白酶-1。它参与胶原蛋白的降解和清除过程，在组织再生、炎症、肿瘤转移等多种生理和病理过程中发挥重要作用。最近的研究表明，MMP1在多种癌症的发生和发展中发挥关键作用，成为了一个重要的治疗靶点。此外，MMP1还与关节炎、动脉粥样硬化等疾病的发生和发展密切相关，成为了一个重要的生物标志物和疾病预测因子。
  • 功能：
    Cleaves collagens of types I, II, and III at one site in the helical domain. Also cleaves collagens of types VII and X. In case of HIV infection, interacts and cleaves the secreted viral Tat protein, leading to a decrease in neuronal Tat's mediated neurotoxicity.
  • 基因功能参考文献：
    1. For MMP-1(-1607) 1G/2G, a significant association was observed using the recessive genetic model. In the subgroup studies, a significant association was observed in the Asian subgroup (OR = 1.68; for the recessive model; and OR = 1.59; for the allelic contrast model) and in the European subgroup (OR = 0.65; for the allelic contrast model). PMID: 30117779
    2. rs1799750 in MMP1 might be a risk factor for rheumatic heart disease in a Han population in Southern China, and individuals carrying the 2G/2G genotype are likely more susceptible to rheumatic heart disease. PMID: 29458338
    3. RHCG Suppresses Tumorigenicity and Metastasis in Esophageal Squamous Cell Carcinoma via Inhibiting NF-kappaB Signaling and MMP1 Expression. PMID: 29290801
    4. Malignant extracellular vesicles carrying MMP1 mRNA facilitate peritoneal dissemination in ovarian cancer. PMID: 28262727
    5. Data suggest that matrix metalloproteinase-1 and neutrophil elastase may be important but heretofore unrecognized players in stimulating uterine contractions at the time of labor, and they may explain why indomethacin delays, but does not prevent, preterm labor because indomethacin inhibits the prostaglandin component but not the protease component of labor. PMID: 28954603
    6. the present study demonstrated that scopoletin inhibited MMP1 and proinflammatory cytokine expression by inhibiting p38 MAPK phosphorylation. PMID: 30015831
    7. Matrix metalloproteinase 1(MMP1), which was downregulated in myelodysplastic syndromes (MDS)-mesenchymal stromal cells (MSCs), was identified as an inhibitory factor of MDS cell proliferation. PMID: 28262842
    8. study to explore the relationship between 2 polymorphisms (MMP-1-755 T/G [rs498186] and MMP-3 A/C [rs632478]) and disc degeneration; no significant association of the MMP-1 polymorphism with disc degeneration was found PMID: 28497435
    9. Expression of GSDMC is increased by UV irradiation in human skin keratinocytes. GSDMC induction contributes to the increase of MMP-1 expression specifically via activation of ERK and JNK pathways. PMID: 29428815
    10. our results suggest that the typical 1G/2G MMP-1 polymorphic site did not play a direct determinant role in breast cancer among Taiwanese women. PMID: 29695550
    11. The maternal and fetal 1G/1G MMP1 gene polymorphisms may be strong genetic markers of preeclampsia, occurring either individually or together. PMID: 29670668
    12. The current study indicated that MMP1 has a key role in the regulation of cervical tumor growth and lymph node metastasis via epithelial mesenchymal transition to a certain extent PMID: 29436615
    13. 2G allele of MMP-1, C allele of MMP-2 and 5A/6A genotype of MMP-3 are associated with susceptibility and disease progression of type 2 diabetic nephropathy PMID: 29883760
    14. this study provides insights into the development of a possible alternative therapy manipulating MMP-1 and the mTOR signaling pathway in Epidermal growth factor receptor-tyrosine kinase inhibitor-resistant lung adenocarcinoma. PMID: 29463039
    15. Specific silencing that targeted AMPK resulted in the enhancement of mobility and contractility and in the production of MMP-1 and MMP-2. PMID: 29122080
    16. MMP-1 genotype may accelerate the development of HIV-associated neurocognitive disorder (HAND) whereas MMP3 -1612 5A5A genotype may reduce the risk of pathogenesis of HAND. PMID: 29358561
    17. s observed a significant increase in the immunoexpression of collagen I and III in patients with DCM and a significant reduction in the immunoexpression of MMP-1 compared with the control group. Also, the collagen I and TIMP-1 expression indicated a positive linear correlation and respectively a negative linear relationship with collagen III and MMP-1. PMID: 29250654
    18. findings suggest that the polymorphic genotypes at MMP-1 promoter -1607 may play a major role in determining personal cancer susceptibility for prostate cancer in Taiwan PMID: 29970511
    19. No significant association was found between MMP-1 (-16071G/2G, -519A/G) variants and obesity. PMID: 29317790
    20. Reduced expression of types I and III collagen and TIMP-1 as well as the increased expression of MMP-1 and MMP-8 in the anterior vaginal wall tissues play important roles in the onset of pelvic organ prolapse. PMID: 29263043
    21. The results of the present study suggested that miR93 regulated MMP1 and collagen I expression in fibroblasts via calpain2. miR93 mediated collagen expression in stress urinary incontinence via calpain2. PMID: 29115452
    22. This study demonstrates that matrix remodeling caused by matrix metalloproteinase (MMP)-1 regulates phenotypic activities and modulates radiosensitivity of hepatocarcinoma cells exclusively in a 3D matrix. In response to MMP-1, liver cancer cells undergo active proliferation, downregulation of E-cadherin, and the loss of detoxification capacity. PMID: 28683186
    23. Results suggested that miR-361 directly inhibited MMP-1 expression by binding to its 3'-UTR suppressing breast cancer cells invasion and metastasis. PMID: 29132384
    24. Review/Meta-analysis: MMP-1 -1607 1G/2G and MMP-12 -82 A/G gene polymorphisms are associated with ischemic stroke risk. PMID: 28958661
    25. Results show that head and neck squamous cell carcinoma tumors with low P120CTN and PI3K pathway mutations have higher levels of MMP1 compared to tumors with high P120CTN and no PI3K pathway mutations demonstrating that P120CTN downregulation and PIK3CA mutations promote MMP1-driven invasion. PMID: 28637905
    26. MMP-1 (and MMP-2 and MMP-3) are independently associated with markers of arterial stiffening in patients with type 1 diabetes. PMID: 29070037
    27. There was no statistically significant difference in the distribution of the MMP1-1607dupG (rs1799750) polymorphism between patients with unstable angina and the control group. PMID: 29044936
    28. PTEN inhibits replicative senescence-induced MMP-1 expression by regulating NOX4-mediated reactive oxygen species in human dermal fibroblasts. PMID: 28557373
    29. None of the studied polymorphisms showed statistically significant association with the risk of varicose veins of the lower extremities. PMID: 28944430
    30. Matrix metalloproteinase 1 rs1799750 polymorphism was significantly associated with nasopharyngeal carcinoma risk in the subgroup analysis by smoking, a marginally significant association was found in non-smokers. PMID: 29096757
    31. Studied the association between the matrix metalloproteinase-1 (MMP-1) 1607 1G/2G polymorphism and susceptibility to spinal tuberculosis in Southern Chinese Han population. PMID: 28129430
    32. MMP1 was consistently and significantly upregulated in the induced pluripotent stem cells-derived endothelia from autosomal dominant polycystic kidney disease patients with intracranial aneurysms compared with the patients without intracranial aneurysms in multiple assays, indicating that high serum MMP1 levels may be a novel risk factor. PMID: 27418197
    33. rs1144393 in the MMP1 promoter region can be a predictor of grade >/= 2 RILI in lung cancer patients treated with thoracic radiation. PMID: 27659527
    34. The present results suggested that Icariin may have a chondroprotective effect, exerted through the inhibition of MMP1, MMP3 and MMP13 via MAPK pathways. Therefore, Icariin may have potential as a novel therapeutic strategy for the treatment of osteoarthritis. PMID: 28447732
    35. proteolysis of collagen-rich natural extracellular matrix (ECM), performed uniquely by individual homologous proteases, leads to distinct events that eventually affect overall ECM morphology, viscoelastic properties, and molecular composition. PMID: 27630193
    36. SAA1 can be a potential mediator for UV-induced MMP-1 expression in human skin. PMID: 26900010
    37. collagen II-activated phosphorylated-DDR2 induces CYR61 through activation of transcription factor activator protein 1 (AP-1). The elevated CYR61, in turn, accelerates MMP1 production via ETS1 (ETS proto-oncogene 1). PMID: 27653023
    38. M. tuberculosis infection caused enhanced MMP-1, -9, and miR-223 expression, with inhibited BMAL1 expression. MiR-223 modulated BMAL1 expression via the direct binding of BMAL1 3'-UTR. PMID: 28543681
    39. 20(S)-PPD treatment modulated MMP-1 mRNA expression and the transcriptional activity of activator protein (AP)-1, a major transcription factor of MMP-1. PMID: 28379603
    40. This is the first study to indicate that the genotypes of MMP1 promoter -1607, do not significantly confer susceptibility to lung cancer in a Taiwanese population. PMID: 29277780
    41. Matrix metalloproteinase-1, -8, -9 and the risk of cardiovascular complications in patients with CHD before and after myocardial revascularization. PMID: 29215835
    42. Results show that the expression level of MMP1 and TIMP1 is under the regulation of HAS2 leading to migration, invasion and EMT of oral cancer cells. PMID: 27884164
    43. DDC up-regulated MMP-1 in LX-2 cells through the Akt and ERK/miR-222/ETS-1 pathways. PMID: 27412967
    44. Meta-analysis of existing data showed that rs1799750 may affect individual susceptibility to glaucoma. PMID: 27428613
    45. results suggest that MMP1-1607 1G/2G, MMP3-1171 5A/6A and MMP9-1562 C/T gene polymorphisms have synergistic effect on breast cancer. The interactions of MMPs clinical risk factors such as lymph node involvement has shown a strong correlation and might influence the 5-years survival rate, suggesting their potential role in the breast carcinogenesis PMID: 28961241
    46. The MMP-1 -1607 (2G/1G) and -519 (A/G) polymorphisms may contribute to the onset of osteomyelitis. The 2G/2G genotype carriers had 1.605 times risk of developing osteomyelitis. In addition, frequency of -519GG genotype was obviously higher in case group than that in control group, and GG genotype related to an increased risk of osteomyelitis. Whereas, the -519G allele may be a susceptible factor for osteomyelitis. PMID: 29049163
    47. No differences in the distribution of the MMP-1 variants were found among Tunisian chronic obstructive pulmonary disease patients and healthy controls. Additionally, no significant association was retrieved between clinicopathologic parameters of chronic obstructive pulmonary disease, MMP-1 levels and this polymorphism. PMID: 27412345
    48. In American cutaneous leishmaniasis caused by Leishmania braziliensis infection, MMP1 is regulated by factors other than FLI1, and the influence of IL-6 on MMP1 was independent of its effect on FLI1. PMID: 28119029
    49. MMP1 was produced in the tumor compartment of prostate cancer bone metastases, highlighting its accessibility to KLK4 at this site PMID: 27378148
    50. EREG and MMP-1 were found to be elevated in nasal polyp and uncinate tissues in patients with Chronic rhinosinusitis with nasal polyps. PMID: 28398769

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  • 亚细胞定位：
    Secreted, extracellular space, extracellular matrix.
  • 蛋白家族：
    Peptidase M10A family
  • 数据库链接：

    HGNC: 7155

    OMIM: 120353

    KEGG: hsa:4312

    STRING: 9606.ENSP00000322788

    UniGene: Hs.83169