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  4. Human Matrix-remodeling-associated protein 8(MXRA8) ELISA kit

Human Matrix-remodeling-associated protein 8(MXRA8) ELISA kit

  • 中文名称：
    人基质重塑关联蛋白8(MXRA8)酶联免疫试剂盒
  • 货号：
    CSB-EL015257HU
  • 规格：
    96T/48T
  • 价格：
    ￥3600/￥2500
  • 其他：

产品详情

  • 产品描述：

    This Human MXRA8 ELISA Kit was designed for the quantitative measurement of Human MXRA8 protein in serum, plasma, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 0.156 ng/mL-10 ng/mL and the sensitivity is 0.039 ng/mL.

  • 别名：
    Limitrin ELISA Kit; Matrix remodelling associated 8 ELISA Kit; Matrix-remodeling-associated protein 8 ELISA Kit; Mxra8 ELISA Kit; MXRA8_HUMAN ELISA Kit
  • 缩写：
    MXRA8
  • Uniprot No.：
  • 种属：
    Homo sapiens (Human)
  • 样本类型：
    serum, plasma, tissue homogenates
  • 检测范围：
    0.156 ng/mL-10 ng/mL
  • 灵敏度：
    0.039 ng/mL
  • 反应时间：
    1-5h
  • 样本体积：
    50-100ul
  • 检测波长：
    450 nm
  • 研究领域：
    Cell Biology
  • 测定原理：
    quantitative
  • 测定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%
    Three samples of known concentration were tested in twenty assays to assess.
  • 线性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of human MXRA8 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    　 Sample Serum(n=4)
    1:1 Average % 87
    Range % 81-92
    1:2 Average % 109
    Range % 101-113
    1:4 Average % 92
    Range % 85-97
    1:8 Average % 91
    Range % 83-95
  • 回收率：
    The recovery of human MXRA8 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample Type Average % Recovery Range
    Serum (n=5) 91 82-94
    EDTA plasma (n=4) 103 94-106
  • 标准曲线：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    ng/ml OD1 OD2 Average Corrected
    10 2.306 2.217 2.262 2.150
    5 1.543 1.523 1.533 1.421
    2.5 0.952 1.001 0.977 0.865
    1.25 0.619 0.600 0.610 0.498
    0.625 0.363 0.343 0.353 0.241
    0.312 0.259 0.252 0.256 0.144
    0.156 0.174 0.181 0.178 0.066
    0 0.110 0.114 0.112 　
  • 数据处理：
  • 货期：
    3-5 working days

引用文献

产品评价

靶点详情

  • 功能：
    Transmembrane protein which can modulate activity of various signaling pathways, probably via binding to integrin ITGAV:ITGB3. Mediates heterophilic cell-cell interactions in vitro. Inhibits osteoclastogenesis downstream of TNFSF11/RANKL and CSF1, where it may function by attenuating signaling via integrin ITGB3 and MAP kinase p38. Plays a role in cartilage formation where it promotes proliferation and maturation of growth plate chondrocytes. Stimulates formation of primary cilia in chondrocytes. Enhances expression of genes involved in the hedgehog signaling pathway in chondrocytes, including the hedgehog signaling molecule IHH; may also promote signaling via the PTHLH/PTHrP pathway. Plays a role in angiogenesis where it suppresses migration of endothelial cells and also promotes their apoptosis. Inhibits VEGF-induced activation of AKT and p38 MAP kinase in endothelial cells. Also inhibits VTN (vitronectin)-mediated integrin ITGAV:ITGB3 signaling and activation of PTK2/FAK. May play a role in the maturation and maintenance of the blood-brain barrier.; (Microbial infection) Contributes to arthritogenic alphavirus pathogenesis and acts as a receptor for these viruses.
  • 基因功能参考文献：
    1. exogenous expression of DICAM in human umbilical vein endothelial cells suppressed angiogenesis in vitro Matrigel and in vivo plug assays, and conversely, DICAM knockdown enhanced angiogenesis PMID: 23386276
    2. MXRA8 is a target gene of the BACH1 transcription factor according to ChIP-seq analysis in HEK 293 cells. PMID: 21555518
    3. new member of the transmembrane-type immunoglobulin superfamily and designated limitrin, localizing selectively to glia limitans in mouse brain PMID: 14603461
  • 亚细胞定位：
    Cell membrane; Single-pass type I membrane protein. Cell junction, tight junction. Cytoplasm. Cell projection, cilium membrane. Nucleus.
  • 组织特异性：
    Detected in endothelial cells in mammary tissue, in both large vessels (left internal mammary artery) and small capillaries (vasa vasorum of the adventitia).
  • 数据库链接：

    HGNC: 7542

    OMIM: 617293

    KEGG: hsa:54587

    STRING: 9606.ENSP00000307887

    UniGene: Hs.515687