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  4. Human N-terminal pro-brain natriuretic peptide,NT-proBNP ELISA KIT

Human N-terminal pro-brain natriuretic peptide,NT-proBNP ELISA KIT

  • 中文名称：
    人N端前脑钠素(NT-proBNP)酶联免疫试剂盒
  • 货号：
    CSB-E05152h
  • 规格：
    96T/48T
  • 价格：
    ￥3600/￥2500
  • 其他：

产品详情

  • 产品描述：
        N-末端B型利钠肽前体（NT-proBNP），是一种由心脏细胞合成的肽类激素，在心脏负荷增加时释放到血液中。它是B型利钠肽的前体，与心力衰竭密切相关。NT-proBNP的含量可以用于诊断和评估心力衰竭。在健康人群中，NT-proBNP的水平通常较低，而在心力衰竭患者中则通常较高。
        j9九游会登录入口首页生物所提供的Human N-terminal pro-brain natriuretic peptide,NT-proBNP ELISA KIT属于ELISA检测试剂盒，采用双抗夹心法定量检测人血清、血浆、组织匀浆样本中的NT-proBNP，其灵敏度为0.216 ng/mL，检测范围为0.313 ng/ml-20 ng/ml。
  • 缩写：
    NT-proBNP
  • 种属：
    Homo sapiens (Human)
  • 样本类型：
    serum, plasma, tissue homogenates
  • 检测范围：
    0.313 ng/ml-20 ng/ml
  • 灵敏度：
    0.216 ng/mL
  • 反应时间：
    1-5h
  • 样本体积：
    50-100ul
  • 检测波长：
    450 nm
  • 研究领域：
    Cardiovascular
  • 测定原理：
    quantitative
  • 测定方法：
    Sandwich
  • 精密度：

     

    Intra-assay Precision (Precision within an assay): CV%<8%

    Three samples of known concentration were tested twenty times on one plate to assess.

    Inter-assay Precision (Precision between assays):CV%<10%

    Three samples of known concentration were tested in twenty assays to assess.

     

  • 线性度：

     

    To assess the linearity of the assay, samples were spiked with high concentrations of human NT-proBNP in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.

     

  • 标准曲线：

     

    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

     

  • 本试剂盒所含材料：
      • An assay plate --- The 96-well plate is composed of 12 x 8 strip plates and can be taken apart.
      • Two bottles of lyophilized standard --- Reconstitute one bottle of the standard with sample diluent at dilution series and draw the standard curve.
      • Biotin-labeled NT-proBNP antibody (100 x concentrate) 1 x 120 μl --- Act as the detection antibody and need to be diluted before use.
      • HRP-avidin (100 x concentrate) 1 x 120 μl --- Catalyze the TMB substrate for color development and need to be diluted before use.
      • Biotin-antibody Diluent 1 x 15 ml --- Dilute the Biotin-antibody.
      • HRP-avidin Diluent 1 x 15 ml --- Dilute the HRP-avidin.
      • Sample Diluent 1 x 50 ml --- Reconstitute the standard and dilute the sample to an appropriate concentration.
      • Wash Buffer (25 x concentrate) 1 x 20 ml --- Wash away the unbound solution and non-specific substances.
      • TMB Substrate 1 x 10 ml --- Act as the chromogenic agent. TMB interacts with HRP, eliciting the solution turns blue.
      • Stop Solution 1 x 10 ml --- Add stop solution and stop the color development. The solution color immediately turns from blue to yellow.
      • Four Adhesive Strips(For 96 wells) --- Seal the microtiter plate when incubation.
      • An instruction manual

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  • 本试剂盒不含材料：
      • A microplate reader capable of measuring absorbance at 450 nm, with the correction wavelength set at 540 nm or 570 nm.
      • An incubator can provide stable incubation conditions up to 37°C±5°C.
      • Centrifuge
      • Vortex
      • Squirt bottle, manifold dispenser, or automated microplate washer
      • Absorbent paper for blotting the microtiter plate
      • 50-300ul multi-channel micropipette
      • Pipette tips
      • Single-channel micropipette with different ranges
      • 100ml and 500ml graduated cylinders
      • Deionized or distilled water
      • Timer
      • Test tubes for dilution

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  • 数据处理：
  • 货期：
    3-5 working days

产品评价

靶点详情

  • 最新研究进展：
    NT-proBNP，全名为N末端B型利钠肽前体，是一种由心脏细胞合成的激素分子。最近的研究表明，NT-proBNP可以作为心衰的标志物，可用于心衰的诊断、预测和治疗监测。此外，NT-proBNP还参与了心血管疾病、肾脏疾病和糖尿病等多种疾病的发生和发展。