Human Nesfatin-1 ELISA Kit

产品详情

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Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

产品描述：

This human nesfatin-1 ELISA kit employs the quantitative sandwich enzyme immunoassay technique to measure the levels of human nesfatin-1 in different samples, including serum, plasma, or tissue homogenates. The enzyme-substrate chromogenic reaction is also used to amplify the signal and quantify the levels of the analyte through the intensity of the colored product. The color intensity positively correlates with the amount of nesfatin-1 bound in the initial step.

Nesfatin-1 is a potent anorexigenic peptide that is processed from the precursor-peptide NEFA/nucleobindin 2 (NUCB2) and is involved in the modulation of homeostatic feeding. In the hypothalamus and brainstem, nesfatin-1 recruits the oxytocin, the melancortin, and other systems to relay its anorexigenic effects. In addition to anorexigenic properties, nesfatin-1 has also been found to perform other important functions, especially associated with energy homeostasis. such as energy expenditure and glucose homeostasis. Nesfatin-1 also has pleiotropic effects on the level of cardiovascular and digestive systems, as well as being implicated in stress response, sleep, and reproduction.
缩写：

Nesfatin-1
种属：

Homo sapiens (Human)
样本类型：

serum, plasma, tissue homogenates
检测范围：

31.25 pg/mL-2000 pg/mL
灵敏度：

7.81 pg/mL
反应时间：

1-5h
样本体积：

50-100ul
检测波长：

450 nm
研究领域：

Others
测定原理：

quantitative
测定方法：

Sandwich

精密度：

线性度：

To assess the linearity of the assay, samples were spiked with high concentrations of human Nesfatin-1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:5	Average %	96
	Range %	80-105
1:10	Average %	99
	Range %	91-111
1:20	Average %	104
	Range %	92-112
1:40	Average %	93
	Range %	86-100

回收率：

The recovery of human Nesfatin-1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	94	89-98
EDTA plasma (n=4)	97	90-107

标准曲线：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.