Human Pancreatic Amylase,PAMY ELISA Kit

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产品详情

产品描述：

This Human AMY2A ELISA Kit was designed for the quantitative measurement of Human AMY2A protein in serum, plasma, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 47 mU/mL-3000 mU/mL and the sensitivity is 11.75 mU/mL.
别名：

AMY2A ELISA Kit; Pancreatic alpha-amylase ELISA Kit; PA ELISA Kit; EC 3.2.1.1 ELISA Kit; 1,4-alpha-D-glucan glucanohydrolase ELISA Kit
缩写：

AMY2A
Uniprot No.：

P04746
种属：

Homo sapiens (Human)
样本类型：

serum, plasma, tissue homogenates
检测范围：

47 mU/mL-3000 mU/mL
灵敏度：

11.75 mU/mL
反应时间：

1-5h
样本体积：

50-100ul
检测波长：

450 nm
研究领域：

Metabolism
测定原理：

quantitative
测定方法：

Sandwich

精密度：

Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.

线性度：

To assess the linearity of the assay, samples were spiked with high concentrations of human PAMY in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	95
Range %	89-99
1:2	Average %	102
Range %	96-107
1:4	Average %	94
Range %	87-98
1:8	Average %	86
Range %	81-92

回收率：

The recovery of human PAMY spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	98	91-103
EDTA plasma (n=4)	102	96-105

标准曲线：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.