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  4. Human Parathyroid hormone,PTH ELISA Kit

Human Parathyroid hormone,PTH ELISA Kit

  • 中文名称：
    人甲状旁腺激素(PTH)酶联免疫试剂盒
  • 货号：
    CSB-E06934h
  • 规格：
    96T/48T
  • 价格：
    ￥3600/￥2500
  • 其他：

产品详情

  • 产品描述：

    This Human PTH ELISA Kit was designed for the quantitative measurement of Human PTH protein in serum, plasma, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 62.5 pg/mL-4000 pg/mL and the sensitivity is 15.6 pg/mL.

  • 别名：
    hPTH ELISA Kit; Parathormone ELISA Kit; Parathyrin ELISA Kit; Parathyroid hormone 1 ELISA Kit; Parathyroid hormone ELISA Kit; Prepro PTH ELISA Kit; Preproparathyroid hormone ELISA Kit; PTH ELISA Kit; PTH1 ELISA Kit; PTH1 receptor ELISA Kit; PTH1R ELISA Kit; PTHR ELISA Kit; PTHR1 ELISA Kit; PTHY_HUMAN ELISA Kit
  • 缩写：
  • Uniprot No.：
  • 种属：
    Homo sapiens (Human)
  • 样本类型：
    serum, plasma, tissue homogenates
  • 检测范围：
    62.5 pg/mL-4000 pg/mL
  • 灵敏度：
    15.6 pg/mL
  • 反应时间：
    1-5h
  • 样本体积：
    50-100ul
  • 检测波长：
    450 nm
  • 研究领域：
    Signal Transduction
  • 测定原理：
    quantitative
  • 测定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%
    Three samples of known concentration were tested in twenty assays to assess.
  • 线性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of human PTH in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    SampleSerum(n=4)
    1:1Average %89
    Range %85-93
    1:2Average %104
    Range %97-107
    1:4Average %95
    Range %88-99
    1:8Average %100
    Range %95-103
  • 回收率：
    The recovery of human PTH spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample TypeAverage % RecoveryRange
    Serum (n=5) 9489-97
    EDTA plasma (n=4)110104-113
  • 标准曲线：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    pg/mlOD1OD2AverageCorrected
    40002.674 2.598 2.636 2.523
    20002.165 2.134 2.150 2.037
    10001.348 1.333 1.341 1.228
    5000.739 0.754 0.747 0.634
    2500.347 0.334 0.341 0.228
    1250.282 0.278 0.280 0.167
    62.50.189 0.183 0.186 0.073
    00.112 0.114 0.113
  • 本试剂盒所含材料：
    • A micro ELISA plate --- The 96-well plate has been pre-coated with an anti-human PTH antibody. This dismountable microplate can be divided into 12 x 8 strip plates.
    • Two vials lyophilized standard ---Dilute a bottle of the standard at dilution series, read the OD values, and then draw a standard curve.
    • One vial Biotin-labeled PTH antibody (100 x concentrate) (120 μl/bottle) ---Act as the detection antibody.
    • One vial HRP-avidin (100 x concentrate) (120 μl/bottle) ---Bind to the detection antibody and react with the TMB substrate to make the solution chromogenic.
    • One vial Biotin-antibodyDiluent (15 ml/bottle) ---Dilute the Biotin-antibody.
    • One vial HRP-avidin Diluent (15 ml/bottle) ---Dilute the HRP-avidin solution.
    • One vial Sample Diluent (50 ml/bottle)---Dilute the sample to an appropriate concentration.
    • One vial Wash Buffer (25 x concentrate) (20 ml/bottle) ---Wash away unbound or free substances.
    • One vial TMB Substrate (10 ml/bottle) ---Act as the chromogenic agent. TMB interacts with HRP, eliciting the solution turns blue.
    • One vial Stop Solution (10 ml/bottle) ---Stop the color reaction. The solution color immediately turns from blue to yellow.
    • Four Adhesive Strips (For 96 wells) --- Cover the microplate when incubation.
    • An instruction manual

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  • 本试剂盒不含材料：
    • A microplate reader capable of measuring absorbance at 450 nm, with the correction wavelength set at 540 nm or 570 nm.
    • An incubator can provide stable incubation conditions up to 37°C±5°C.
    • Centrifuge
    • Vortex
    • Squirt bottle, manifold dispenser, or automated microplate washer
    • Absorbent paper for blotting the microtiter plate
    • 50-300ul multi-channel micropipette
    • Pipette tips
    • Single-channel micropipette with different ranges
    • 100ml and 500ml graduated cylinders
    • Deionized or distilled water
    • Timer
    • Test tubes for dilution

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  • 数据处理：
  • 货期：
    3-5 working days

产品评价

靶点详情

  • 功能：
    PTH elevates calcium level by dissolving the salts in bone and preventing their renal excretion. Stimulates [1-14C]-2-deoxy-D-glucose (2DG) transport and glycogen synthesis in osteoblastic cells.
  • 基因功能参考文献：
    1. Among subjects with preserved renal function, PTH levels were possibly associated with metabolic syndrome in females, whereas vitamin D levels exhibited a possible link to metabolic syndrome in males. PMID: 29348466
    2. Data suggest that, in pregnant women with largely sufficient calcium intakes (via diet and dietary supplements), serum 25-hydroxyvitamin D (total of 25[OH]D2 and 25[OH]D3) is important for maintaining normal serum PTH levels; this study was conducted in group of white-skinned women at northern latitudes in Ireland. PMID: 30018262
    3. In conclusion, Fetuin A, vitamin D, and intact PTH levels were all associated with sarcopenia in this geriatric population. Among them, intact PTH specifically indicates patients with sarcopenic left ventricular hypertrophy. PMID: 28112206
    4. Dkk-1 and PTH levels in psoriatic arthritis are lower than healthy female controls, in contrast with rheumatoid arthritis, in which they are increased PMID: 28634697
    5. Scientific evidence of genetic association of serum PTH level among individuals with different pathologic conditions remains deficient and published results provide weak evidence (review and meta-analysis). PMID: 29794776
    6. Clinical data on secondary hyperparathyroidism, mainly derived from patients with chronic kidney disease, indicate a potential inverse association between leptin (LEP) and parathyroid hormone (PTH) in some, but not all studies. [REVIEW] PMID: 28730419
    7. Parathyroid hormone controls bone and kidney homeostasis via GNAS and Gq-G11 heterotrimeric G proteins. (Review) PMID: 28363951
    8. Patients with chronic kidney disease undergoing parathyroidectomy for primary hyperparathyroidism have similar intraoperative parathyroid hormone degradation kinetics to those with normal kidney function. PMID: 29128188
    9. PTH levels are significantly higher in untreated sustained hypertension patients than white-coat hypertension patients and normotensive subjects. PMID: 29176783
    10. results revealed that PTH treatment on HCs, either continuous or pulsatile, does not exhibit any positive effect, and indicates that exogenous PTH administration after fracture has no effect on HCs. PTH may not have a positive effect at the fracture site during the early stage of fracture healing in which haematoma formation occurs. PMID: 24039091
    11. Pre-incubation of muscle fibers or myotubes with physiological concentrations of PTH, concentration-dependently reduced uptake of labelled 25-hydroxyvitamin D3. PMID: 28104493
    12. Baseline PTH level was not associated with changes in frailty status in men. PMID: 28609827
    13. Elevated PTH induces the transition of endothelial cells to chondrogenic cells via endothelial-mesenchymal transition, possibly mediated by the nuclear translocation of beta-catenin. PMID: 27582099
    14. Cys mutation at the 25th residue of hPTH(1-34) may result in a high bone mass phenotype. PMID: 27300576
    15. Common genetic variants located near genes involved in vitamin D metabolism and calcium and renal phosphate transport associated with differences in circulating parathyroid hormone concentrations PMID: 27927781
    16. PTH pretreatment prevented TGF-beta1 and high glucose-induced Smad2/3 phosphorylation and consequent upregulation of fibronectin and type IV collagen within 4 h. PMID: 27530924
    17. FGFR1c and PTHR signaling pathways converge on NHERF1 to inhibit PTH- and FGF23-sensitive phosphate transport and down-regulate NPT2A. PMID: 27432882
    18. The present study supports the independent pathogenic effect of rs6254GA polymorphism on the development and severity of bone mineral density complications in patients with asymptomatic but not normocalcemic hyperparathyroidism. PMID: 27756092
    19. In patients receiving dual antiplatelet therapy for coronary artery disease, higher PTH levels are associated with an increased ADP-mediated platelet reactivity and suboptimal response to clopidogrel. PMID: 27086085
    20. Parathyroid hormone gene rs6256 variants are not associated with susceptibility to colorectal cancer PMID: 25124570
    21. PTH levels were significantly higher in patients with aldosterone producing adenomas. PMID: 26304960
    22. Although there was a trend for a negative association in women, no statistically significant association was found between endogenous PTH and knee osteoarthritis. PMID: 25879737
    23. The purpose of this study was to investigate the prevalence of adenomatous colon polyps (ACP) as they occur in subjects with diabetes mellitus (DM) and chronic kidney disease. PMID: 27993873
    24. PTH [parathyroid hormone]together with other markers of heart failure may provide valuable information both in the diagnosis and staging of heart failure syndromes PMID: 27546695
    25. Patients with a serum PTH decrease to low values after 1 year of hemodialysis treatment are at high risk of short-term cardiovascular death. PMID: 26880460
    26. a cell model of human dermal fibroblasts in order to investigate the functions of sclerostin, is reported. PMID: 26851122
    27. recombinant human klotho inhibits the expression of 1-OH by PTH both in vitro and in vivo. PMID: 26287968
    28. results suggest that NO-mediated vasorelaxation plays partly a role in the anabolic action of PTH on cortical bone PMID: 26834008
    29. In this large community-based cohort, PTH levels, overall, were not independently associated with the risk of hypertension. However, we found some evidence that PTH may be associated with hypertension in blacks. PMID: 26867053
    30. The objective of this article is to investigate the effect of renin-angiotensin system inhibitors (RASIs) on intact parathyroid hormone (iPTH) levels in continuous ambulatory peritoneal dialysis (CAPD) patients. PMID: 25271253
    31. PTH does not contribute to the occurrence of metabolic components of obesity, but there is a positive correlation between 25(OH)D and HDL-C. PMID: 26451492
    32. Data suggest that effects of PTH on bone remodeling are mediated not only by osteoblasts/osteocytes but also by T-lymphocytes in bone marrow; T-lymphocytes regulate differentiation/life span of stromal cells and their responsiveness to PTH. [REVIEW] PMID: 26662934
    33. Serum secreted i-PTH level might not be predictable by a total mass of parathyroid glands or by their blood supply. PMID: 26997379
    34. A Homozygous [Cys25]PTH(1-84) Mutation That Impairs PTH/PTHrP Receptor Activation Defines a Novel Form of Hypoparathyroidism. PMID: 25891861
    35. Study indicates significant association between specific PTH gene promoter region variants and altered levels of 25(OH)D and vitamin D deficiency among specific nationals. PMID: 26339419
    36. Elevation of PTH, unlike vitamin D, is independently associated with Chronic Obstructive Pulmonary Disease (COPD) severity, and may be a better biomarker for COPD. PMID: 26398210
    37. Abnormal diurnal patterns of PTH are associated with sustained mild hypercalcemia in nondialyzed chronic kidney disease patients. PMID: 27012036
    38. These data indicate that ephrinB2/EphB4 signaling within the osteoblast lineage is required for late stages of osteoblast differentiation. PMID: 23165727
    39. Serum 25(OH)D levels were inversely associated with serum PTH and bone mineral density. PMID: 23045165
    40. Elevated parathyroid hormone serum concentrations might have a role in colorectal cancer development as indicated by higher rates of adenomas, specifically with dysplasia, in women. PMID: 26021763
    41. the iPTH level in ESRD patients carrying VDR BsmI Bb genotype was higher than that in ESRD patients carrying bb genotype in overall populations and in Caucasians PMID: 25007156
    42. The identification of PTH and other hormonal resistances implies to look for the genetic disorder supporting the metabolic disorder--{REVIEW} PMID: 25913526
    43. Intraoperative PTH monitoring during parathyroidectomy to determine PTH clearance proved to be a feasible marker for adequacy and safety of surgery and "cure". PMID: 25241609
    44. Data suggest serum PTH level is independently associated with bone mineral density (BMD) in vitamin D-sufficient women and vitamin D-insufficient women; no association was found in men; high serum PTH is detrimental on BMD in postmenopausal women. PMID: 25242259
    45. In a racially/ethnically diverse population without prevalent cardiovascular disease, higher serum PTH concentration was associated with increased left ventricular mass and increased risk of incident heart failure. PMID: 25468653
    46. In a large community-based sample of elderly, calcium was independently associated with increased arterial stiffness, and PTH independently to intra-arterial peripheral and calculated central blood pressures. PMID: 25562577
    47. Suggest that measurement of iPTH may be useful for assessment of mineral bone disorder in patients with chronic kidney disease. PMID: 26299086
    48. A detailed characterization of in vitro generated amyloid fibrils from human parathyroid hormone (hPTH(1-84)). PMID: 25554227
    49. salt bridge between Arg-20 on parathyroid hormone (PTH) and Asp-137 on the PTH1 receptor is essential for full affinity. PMID: 25218037
    50. A panel of easily assessable determinants could account for both a substantial proportion of PTH variance and the occurrence of secondary hyperparathyroidism. PMID: 24202062

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  • 相关疾病：
    Hypoparathyroidism, familial isolated (FIH)
  • 亚细胞定位：
    Secreted.
  • 蛋白家族：
    Parathyroid hormone family
  • 数据库链接：

    HGNC: 9606

    OMIM: 146200

    KEGG: hsa:5741

    STRING: 9606.ENSP00000282091

    UniGene: Hs.37045