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  4. Human Placental alkaline phosphatase,PLAP ELISA Kit

Human Placental alkaline phosphatase,PLAP ELISA Kit

  • 中文名称：
    人胎盘碱性磷酸酶(PLAP)酶联免疫试剂盒
  • 货号：
    CSB-E09160h
  • 规格：
    96T/48T
  • 价格：
    ￥3200/￥2500
  • 其他：

产品详情

  • 产品描述：

    This Human ALPP ELISA Kit was designed for the quantitative measurement of Human ALPP protein in serum, plasma, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 84 pg/mL-2000 pg/mL and the sensitivity is 34 pg/mL.

  • 别名：
    ALPP; PLAP; Alkaline phosphatase, placental type; Alkaline phosphatase Regan isozyme; Placental alkaline phosphatase 1; PLAP-1
  • 缩写：
  • Uniprot No.：
  • 种属：
    Homo sapiens (Human)
  • 样本类型：
    serum, plasma, tissue homogenates
  • 检测范围：
    84 pg/mL-2000 pg/mL
  • 灵敏度：
    34 pg/mL
  • 反应时间：
    1-5h
  • 样本体积：
    50-100ul
  • 检测波长：
    450 nm
  • 研究领域：
    Tags & Cell Markers
  • 测定原理：
    quantitative
  • 测定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<10%      
    Three samples of known concentration were tested twenty times on one plate to assess.  
    Inter-assay Precision (Precision between assays): CV%<15%      
    Three samples of known concentration were tested in twenty assays to assess.    
                 
  • 线性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of human PLAP in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    　 Sample Serum(n=4)  
    1:1 Average % 92  
    Range % 85-98  
    1:2 Average % 96  
    Range % 88-100  
    1:4 Average % 95  
    Range % 89-100  
    1:8 Average % 97  
    Range % 91-101  
  • 回收率：
    The recovery of human PLAP spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample Type Average % Recovery Range  
    Serum (n=5) 98 93-104  
    EDTA plasma (n=4) 95 91-101  
                 
                 
  • 标准曲线：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    pg/ml OD1 OD2 Average Corrected  
    2000 2.012 1.983 1.998 1.952  
    1000 1.338 1.341 1.340 1.294  
    500 0.775 0.828 0.802 0.756  
    167 0.357 0.349 0.353 0.307  
    84 0.224 0.235 0.230 0.184  
    0 0.047 0.045 0.046 　  
  • 数据处理：
  • 货期：
    3-5 working days

产品评价

问答及客户评论

■ 常见问题解答
Q:

I would like to know whether the CSB-E09160h - Cusabio ELISA kit (quotation as attached) is able to quantify recombinant proteins (non-native)?

A:
Thanks for your inquiry. As in recombinant protein ,there is difference on expression system ,expression region and posttranslational modification,ect,we are not sure if recombinant proteins can be detected out.If the conformation of your recombinant proteins is same with native proteins ,then our kit can quantify it.

靶点详情

  • 功能：
    Alkaline phosphatase that can hydrolyze various phosphate compounds.
  • 基因功能参考文献：
    1. This meta-analysis suggests that high serum ALP level is obviously associated with lower OS rate in patients with osteosarcoma, and it is an effective biomarker of prognosis. PMID: 29970708
    2. Concentrations of PLAP were elevated in gingival crevicular fluid of patients with pre-eclampsia. PMID: 26988336
    3. SALL4 also outperformed PLAP on a small sample of cytology blocks. Although SALL4 is not entirely specific, it is a highly sensitive marker with strong diffuse nuclear reactivity in the majority of MGCTs in the posttreatment setting, at significantly higher levels than PLAP PMID: 25906119
    4. Quantum-mechanical computational methods were employed to study the catalytic mechanism of human placental AP (PLAP). An active-site model was used, constructed on the basis of the X-ray crystal structure of the enzyme. PMID: 25409280
    5. term placental explants, but not their conditioned medium, can de-phosphorylate IGFBP-1 through the action of placental alkaline phosphatase PMID: 24856042
    6. Anti-PLAP antibodies may serve as a modular building blocks for the development of targeted therapeutic products, armed with cytotoxic drugs, radionuclides or cytokines as payloads. PMID: 24247025
    7. A candidate gene, ALPP, encoding the placental alkaline phosphatase, was identified as being potentially involved in recurrent spontaneous abortion. PMID: 24296104
    8. The objective of the following study was to record the specificity and sensitivity of alpha5(IV) loss, smoothelin expression and PLAP expression as markers of gastrointestinal smooth muscle neoplasms PMID: 24043717
    9. Data indicate that p180 is required for the efficient targeting of placental alkaline phosphatase (ALPP) mRNA to the endoplasmic reticulum (ER). PMID: 24019514
    10. Calculation of the electrostatic potentials within the active site of human placental alkaline phosphatase also suggests that the local positive electrostatic environment may account for its capability to distinguish various substrates PMID: 21910833
    11. High serum alkaline phosphatase cooperating with MMP-9 is associated with metastasis in patients with primary osteosarcoma. PMID: 22333159
    12. PLAP exerts a positive effect on DNA replication and acts as a proliferative factor in trophoblastic cells. PMID: 21868091
    13. The proximity of undifferentiated gonadal tissue with the tumors as well as the immunostaining patterns (PLAP+, OCT3/4+, and CD117/KIT+) suggests that germ cells found in them are a risk factor for gonadal tumors. PMID: 21692598
    14. the catalytic mechanism of human placental alkaline phosphatase PMID: 21939286
    15. Data show that serum alkaline phosphatase, Gleason score, and intensity of bone metastasis are important and statistically significant prognostic factors, and affects time to progression and life time. PMID: 19450995
    16. at a certain time point during adrenocortical development, some fetal zone cells survive owing to defective apoptosis and develop into childhood ACT, maintaining some characteristics of the embryonal period, such as PLAP expression PMID: 21516013
    17. Serum total calcium (r -0.1362, p<0.001), serum inorganic phosphate (r -0.45, p<0.001) and serum alkaline phosphatase (r -0.5587, p<0.001) have shown inverse relationship with age. PMID: 20655896
    18. High serum alkaline phosphatase is associated with chronic kidney disease. PMID: 20299338
    19. differential expression of Pl(1) and Pl(2) probably results from linkage disequilibrium with the sequence variation rs2014683G>A in the ALPP gene promoter that was shown to have allele-specific binding patterns to placental nuclear proteins. PMID: 20663553
    20. structure of placental alkaline phosphatase with pNPP contained only p-nitrophenol in three distinct sites, while the structure with 5'-AMP contained the p-nitrophenyl group in two of the sites instead of 5'-AMP PMID: 20693656
    21. Low magnitudes of tensile strain enhance expression of alkaline phosphatase in human osteoblasts. PMID: 19595020
    22. The PLAP D allele contains two amino acid substitutions: P209R (692C>G) and E429G (1352 A>G). PMID: 11857742
    23. Proximity of the protein moiety of a GPI-anchored protein to the membrane surface: a FRET study. PMID: 12081485
    24. the beta-N-acetylglucosaminyl phosphate diester residue is attached to the glycosylphosphatidylinositol anchor of human placental alkaline phosphatase and is a target of the channel-forming toxin aerolysin PMID: 12851398
    25. receptor for Aeromonas sobria hemolysin. PMID: 15715171
    26. analysis of human placental alkaline phosphatase in complex with functional ligands PMID: 15946677
    27. The effect of parity on placental weight and birth weight is examined through a series of birth records from an Indian population in Calcutta. PMID: 16431676
    28. crystal structure of strontium-substituted human placental alkaline phosphatase shows that strontium substitutes the calcium ion with concomitant modification of the metal coordination PMID: 16815919
    29. activity of GPI-anchored enzymes may be modulated by membrane microenvironment features PMID: 18416535
    30. Serum bilirubin, alkaline phosphatase, and aspartate aminotransferase are an efficient set of biochemistries to identify UDCA-treated patients with primary biliary cirrhosis at risk of death or liver transplantation (LT). PMID: 18752324
    31. Elevations of alkaline phosphatase is associated with therapy related pediatric cancer. PMID: 18802949

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  • 亚细胞定位：
    Cell membrane; Lipid-anchor, GPI-anchor.
  • 蛋白家族：
    Alkaline phosphatase family
  • 组织特异性：
    Detected in placenta (at protein level).
  • 数据库链接：

    HGNC: 439

    OMIM: 171800

    KEGG: hsa:250

    STRING: 9606.ENSP00000375881

    UniGene: Hs.284255