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  4. Human Platelet factor 4 variant(PF4V1) ELISA kit

Human Platelet factor 4 variant(PF4V1) ELISA kit

  • 中文名称：
    人血小板因子4变体(PF4V1)酶联免疫试剂盒
  • 货号：
    CSB-EL017810HU
  • 规格：
    96T/48T
  • 价格：
    ￥3600/￥2500
  • 其他：

产品详情

  • 产品描述：

    This Human PF4V1 ELISA Kit was designed for the quantitative measurement of Human PF4V1 protein in serum, plasma, cell culture supernates, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 0.625 ng/mL-40 ng/mL and the sensitivity is 0.156 ng/mL.

  • 别名：
    PF4V1 ELISA Kit; CXCL4V1 ELISA Kit; SCYB4V1 ELISA Kit; Platelet factor 4 variant ELISA Kit; C-X-C motif chemokine 4 variant ELISA Kit; CXCL4L1 ELISA Kit; PF4alt ELISA Kit; PF4var1) [Cleaved into: Platelet factor 4 variant(4-74); Platelet factor 4 variant(5-74); Platelet factor 4 variant(6-74)] ELISA Kit
  • 缩写：
  • Uniprot No.：
  • 种属：
    Homo sapiens (Human)
  • 样本类型：
    serum, plasma, cell culture supernates, tissue homogenates
  • 检测范围：
    0.625 ng/mL-40 ng/mL
  • 灵敏度：
    0.156 ng/mL
  • 反应时间：
    1-5h
  • 样本体积：
    50-100ul
  • 检测波长：
    450 nm
  • 研究领域：
    Others
  • 测定原理：
    quantitative
  • 测定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%
    Three samples of known concentration were tested in twenty assays to assess.
  • 线性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of human PF4V1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    SampleSerum(n=4)
    1:1Average %94
    Range %89-99
    1:2Average %98
    Range %95-103
    1:4Average %102
    Range %94-106
    1:8Average %95
    Range %90-98
  • 回收率：
    The recovery of human PF4V1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample TypeAverage % RecoveryRange
    Serum (n=5) 9289-95
    EDTA plasma (n=4)9996-103
  • 标准曲线：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    ng/mlOD1OD2AverageCorrected
    402.945 3.094 3.020 2.831
    202.266 2.399 2.333 2.144
    101.532 1.497 1.515 1.326
    50.775 0.776 0.776 0.587
    2.50.453 0.454 0.454 0.265
    1.250.369 0.341 0.355 0.166
    0.6250.230 0.250 0.240 0.051
    00.184 0.194 0.189
  • 数据处理：
  • 货期：
    3-5 working days

产品评价

靶点详情

  • 功能：
    Inhibitor of angiogenesis. Inhibitor of endothelial cell chemotaxis (in vitro).
  • 基因功能参考文献：
    1. Fibstatin and CXCL4L1 cooperate to inhibit endothelial cell proliferation, migration and tubulogenesis in vitro. PMID: 23747987
    2. fibstatin and CXCL4L1 have cooperative roles in inhibition of tumor invasion in lymph nodes PMID: 23747987
    3. Structural insight into the novel functional properties of human CXCL4L1. PMID: 23536183
    4. PF-4var/CXCL4L1 predicts outcome in stable coronary artery disease patients with preserved left ventricular function PMID: 22384011
    5. both CXCR3A and CXCR3B are implicated in the chemotactic and vascular effects of CXCL4L1. PMID: 20980681

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  • 亚细胞定位：
    Secreted.
  • 蛋白家族：
    Intercrine alpha (chemokine CxC) family
  • 数据库链接：

    HGNC: 8862

    OMIM: 173461

    KEGG: hsa:5197

    STRING: 9606.ENSP00000226524

    UniGene: Hs.72933