Your Good Partner in Biology Research

  1. 首页
  2. 试剂盒
  3. 酶联免疫试剂盒
  4. Human Platelet receptor Gi24(C10orf54) ELISA kit

Human Platelet receptor Gi24(C10orf54) ELISA kit

  • 中文名称：
    人血小板受体Gi24(C10orf54)酶联免疫试剂盒
  • 货号：
    CSB-EL002961HU
  • 规格：
    96T/48T
  • 价格：
    ￥3600/￥2500
  • 其他：

产品详情

  • 产品描述：

    This Human C10orf54 ELISA Kit was designed for the quantitative measurement of Human C10orf54 protein in serum, plasma, tissue homogenates, cell lysates. It is a Sandwich ELISA kit, its detection range is 0.156 ng/mL-10 ng/mL and the sensitivity is 0.039 ng/mL.

  • 别名：
    B7 H5 ELISA Kit; B7H5 ELISA Kit; C10orf54 ELISA Kit; chromosome 10 open reading frame 54 ELISA Kit; DD1alpha ELISA Kit; GI24 ELISA Kit; GI24_HUMAN ELISA Kit; PD-1H ELISA Kit; PDCD1 homolog ELISA Kit; Platelet receptor Gi24 ELISA Kit; PP2135 ELISA Kit; sisp 1 ELISA Kit; SISP1 ELISA Kit; stress induced secreted protein 1 ELISA Kit; UNQ730/PRO1412 ELISA Kit; V domain Ig suppressor of T cell activation ELISA Kit; V set domain containing immunoregulatory receptor ELISA Kit; V set immunoregulatory receptor ELISA Kit; V type immunoglobulin domain containing suppressor of T cell activation ELISA Kit; VISTA ELISA Kit
  • 缩写：
    C10orf54
  • Uniprot No.：
  • 种属：
    Homo sapiens (Human)
  • 样本类型：
    serum, plasma, tissue homogenates, cell lysates
  • 检测范围：
    0.156 ng/mL-10 ng/mL
  • 灵敏度：
    0.039 ng/mL
  • 反应时间：
    1-5h
  • 样本体积：
    50-100ul
  • 检测波长：
    450 nm
  • 研究领域：
    Cell Biology
  • 测定原理：
    quantitative
  • 测定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%
    Three samples of known concentration were tested in twenty assays to assess.
  • 线性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of human C10orf54 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    　 Sample Serum(n=4)
    1:1 Average % 85
    Range % 83-89
    1:2 Average % 100
    Range % 95-105
    1:4 Average % 84
    Range % 81-89
    1:8 Average % 92
    Range % 88-96
  • 回收率：
    The recovery of human C10orf54 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample Type Average % Recovery Range
    Serum (n=5) 101 98-104
    EDTA plasma (n=4) 99 95-103
  • 标准曲线：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    ng/ml OD1 OD2 Average Corrected
    10 2.366 2.305 2.336 2.213
    5 1.642 1.658 1.650 1.527
    2.5 0.898 0.868 0.883 0.760
    1.25 0.475 0.455 0.465 0.342
    0.625 0.320 0.326 0.323 0.200
    0.312 0.249 0.251 0.250 0.127
    0.156 0.195 0.193 0.194 0.071
    0 0.125 0.120 0.123 　
  • 数据处理：
  • 货期：
    3-5 working days

产品评价

靶点详情

  • 功能：
    Immunoregulatory receptor which inhibits the T-cell response. May promote differentiation of embryonic stem cells, by inhibiting BMP4 signaling. May stimulate MMP14-mediated MMP2 activation.
  • 基因功能参考文献：
    1. Data indicate that VISTA is predominantly expressed and up-regulated in the high-density infiltrated immune cells but minimal in pancreatic cancerous cells. PMID: 29771768
    2. High VISTA expression is associated with colorectal carcinoma. PMID: 30128738
    3. VISTA expression supports immune-complex inflammation in collagen antibody-induced arthritis and VISTA is expressed in human synovium PMID: 29216931
    4. This study is the first to describe the expression of VISTA-expressing lymphocytes in melanoma samples and in the context of acquired resistance to immune checkpoint inhibitors PMID: 28776578
    5. Taken together, the results indicated that the VISTA high and CD8 low group, as an immunosuppressive subgroup, might be associated with a poor prognosis in primary OSCC. These findings indicated that VISTA might be a potential immunotherapeutic target in OSCC treatment. PMID: 28236118
    6. this review describes the functions of VISTA in the context of cancer immunotherapy PMID: 28258694
    7. overexpression of the newly described co-stimulatory molecule, PD1 homologue (PD-1H) in human monocyte/macrophages is sufficient to induce spontaneous secretion of multiple cytokines. PMID: 25279955
    8. p53-induced expression of DD1alpha prevents persistence of cell corpses and ensures efficient generation of precise immune responses in mice. PMID: 26228159
    9. Results suggest that GI24 contributes to tumor-invasive growth in the collagen matrix by augmenting cell surface MT1-MMP. PMID: 20666777

    显示更多

    收起更多

  • 亚细胞定位：
    Cell membrane; Single-pass type I membrane protein.
  • 组织特异性：
    Expressed in spleen. Detected on a number of myeloid cells including CD11b monocytes, CD66b+ neutrophils, at low levels on CD4+ and CD8+ T-cells, and in a subset of NK cells. Not detected on B cells (at protein level). Expressed at high levels in placenta
  • 数据库链接：

    HGNC: 30085

    OMIM: 615608

    KEGG: hsa:64115

    STRING: 9606.ENSP00000378409

    UniGene: Hs.47382