Your Good Partner in Biology Research

  1. 首页
  2. 试剂盒
  3. 酶联免疫试剂盒
  4. Human Protein S100-A4(S100A4) ELISA kit

Human Protein S100-A4(S100A4) ELISA kit

  • 中文名称：
    人S100-A4蛋白(S100A4)酶联免疫试剂盒
  • 货号：
    CSB-EL020632HU
  • 规格：
    96T/48T
  • 价格：
    ￥3600/￥2500
  • 其他：

产品详情

  • 产品描述：

    This Human S100A4 ELISA Kit was designed for the quantitative measurement of Human S100A4 protein in serum, plasma, cell culture supernates, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 0.9 ng/mL-60 ng/mL and the sensitivity is 0.225 ng/mL.

  • 别名：
    18A2 ELISA Kit; 42A ELISA Kit; calcium Placental protein ELISA Kit; Calvasculin ELISA Kit; CAPL ELISA Kit; Fibroblast specific protein 1 (FSP1) ELISA Kit; Fibroblast specific protein 1 ELISA Kit; Fibroblast specific protein ELISA Kit; FSP1 ELISA Kit; Leukemia multidrug resistance associated protein ELISA Kit; Malignant transformation suppression 1 (MTS1) ELISA Kit; Malignant transformation suppression 1 ELISA Kit; Metastasin ELISA Kit; MTS1 ELISA Kit; OTTHUMP00000015467 ELISA Kit; OTTHUMP00000015468 ELISA Kit; P9KA ELISA Kit; PEL98 ELISA Kit; Placental calcium-binding protein ELISA Kit; Protein Mts1 ELISA Kit; Protein S100 A4 ELISA Kit; Protein S100-A4 ELISA Kit; S100 calcium binding protein A4 (calcium protein, calvasculin, metastasin, murine placental homolog) ELISA Kit; S100 calcium binding protein A4 ELISA Kit; S100 calcium-binding protein A4 ELISA Kit; S100a4 ELISA Kit; S10A4_HUMAN ELISA Kit
  • 缩写：
  • Uniprot No.：
  • 种属：
    Homo sapiens (Human)
  • 样本类型：
    serum, plasma, cell culture supernates, tissue homogenates
  • 检测范围：
    0.9 ng/mL-60 ng/mL
  • 灵敏度：
    0.225 ng/mL
  • 反应时间：
    1-5h
  • 样本体积：
    50-100ul
  • 检测波长：
    450 nm
  • 研究领域：
    Signal Transduction
  • 测定原理：
    quantitative
  • 测定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%      
    Three samples of known concentration were tested twenty times on one plate to assess.  
    Inter-assay Precision (Precision between assays): CV%<10%      
    Three samples of known concentration were tested in twenty assays to assess.    
                 
  • 线性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of human S100A4 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    　 Sample Serum(n=4)  
    1:1 Average % 85  
    Range % 80-96  
    1:2 Average % 93  
    Range % 82-101  
    1:4 Average % 97  
    Range % 93-104  
    1:8 Average % 93  
    Range % 86-98  
  • 回收率：
    The recovery of human S100A4 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample Type Average % Recovery Range  
    Serum (n=5) 98 92-105  
    EDTA plasma (n=4) 100 95-106  
                 
                 
  • 标准曲线：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    ng/ml OD1 OD2 Average Corrected  
    60 2.654 2.587 2.621 2.471  
    30 2.216 2.115 2.166 2.016  
    15 1.369 1.406 1.388 1.238  
    7.5 0.689 0.715 0.702 0.552  
    3.75 0.385 0.367 0.376 0.226  
    1.8 0.288 0.279 0.284 0.134  
    0.9 0.216 0.206 0.211 0.061  
    0 0.156 0.144 0.150 　  
  • 数据处理：
  • 货期：
    3-5 working days

产品评价

靶点详情

  • 功能：
    Calcium-binding protein that plays a role in various cellular processes including motility, angiogenesis, cell differentiation, apoptosis, and autophagy. Increases cell motility and invasiveness by interacting with non-muscle myosin heavy chain (NMMHC) IIA/MYH9. Mechanistically, promotes filament depolymerization and increases the amount of soluble myosin-IIA, resulting in the formation of stable protrusions facilitating chemotaxis. Modulates also the pro-apoptotic function of TP53 by binding to its C-terminal transactivation domain within the nucleus and reducing its protein levels. Within the extracellular space, stimulates cytokine production including granulocyte colony-stimulating factor and CCL24 from T-lymphocytes. In addition, stimulates T-lymphocyte chemotaxis by acting as a chemoattractant complex with PGLYRP1 that promotes lymphocyte migration via CCR5 and CXCR3 receptors.
  • 基因功能参考文献：
    1. ERRgamma may exert oncogenic activity mainly associated with aggressiveness of EC by activating S100A4 transcription. PMID: 29901152
    2. S100A4 expression is induced by the TGF-beta pathway,. PMID: 29141874
    3. This meta-analysis showed that overexpression of S100A4 seems to be associated with tumor progression and poor prognosis of PC patients. PMID: 29578167
    4. clusterin promotes growth and invasion in renal cell carcinoma cells in vitro and in vivo through upregulation of S100A4 PMID: 29400663
    5. miR-3189-3p mimics enhanced the effects of the S100A4 siRNA on the inhibition of gastric cancer cell proliferation and migration by targeting CFL2. PMID: 29342841
    6. Urine S100A4 shows the most promise as an lupus nephritis (LN) activity biomarker. PMID: 29065913
    7. S100A4 is up-regulated in cervical adenocarcinomas and squamous cell carcinomas. PMID: 28921916
    8. Network analysis of overlapping genes revealed the effects on tubulins (Tubb2a, Tubb3, Tubb4b), Nfe2l2, S100a4, Cd44, and Nfkb2, all of which are linked to TBI-relevant outcomes, including epileptogenesis and tissue repair PMID: 27530814
    9. s first provide experimental evidence suggesting that FAM107B was downregulated by S100A4 in gastric cancer MGC803 cells. And FAM107B at least partially mediates the biological effect of S100A4 in the cells. PMID: 28675500
    10. MiR-187 could interact with S100A4 3'-UTR. PMID: 29303365
    11. Both the calcium-binding ability and the C-terminal region of S100A4 are important for cancer initiatin cells to sustain their stemness properties and malignancy in head and neck cancers. PMID: 27793047
    12. Studied the role and expression of MIR296 in the metastasis and epithelial-mesenchymal transition of colorectal cancer. Found MIR296 inhibited migration and invasion of tumor cells, as well as suppressed S100A4 abundance in tumor cells. PMID: 28209128
    13. S100A4-expression was associated with poor outcome in early-stage breast cancer, but the low percentage of positive tumors and the modest survival differences imply that the clinical utility in selection of patients for adjuvant treatment is limited. PMID: 28058579
    14. The relative expression of S100A4 mRNA was higher when mod-SHEM was used, although significant differences were detected only when the expression levels were compared with those found in LPCs incubated with IOBA-FBS PMID: 27911610
    15. High S100A4 expression is associated with Mesenchymal Transition in Glioblastoma. PMID: 28807938
    16. our findings demonstrate that S100A4 is post-transcriptionally regulated by tumor suppressor microRNAs PMID: 28423501
    17. findings indicate that Idiopathic pulmonary fibrosis mesenchymal progenitor cells are intrinsically fibrogenic and that S100A4 confers mesenchymal progenitor cells with fibrogenicity. PMID: 28530639
    18. S100a4 is expressed in a limited number of stromal cells throughout the colon. S100A4 was mainly localised in the colorectal tumour-associated stroma, it was also detectable in a limited number of epithelial tumour cells. PMID: 27750112
    19. our results suggest the existence of evolutionarily conserved pathways used by S100A4 to promote metastatic dissemination PMID: 27927689
    20. miR-187-3p inhibits the migration/metastasis and epithelial mesenchymal transition in hepatocellular carcinoma by targeting S100A4 expression. PMID: 27544906
    21. our findings highlight nuclear S100A4 as a candidate therapeutic target in cholangiocarcinoma and establish a mechanistic rationale for the use of low-dose paclitaxel in blocking metastatic progression of cholangiocarcinoma PMID: 27328733
    22. The increased content of collagen fibers and overexpression of S100A4 and P53 may play an important role in myocardial fibrosis of HCM, and they can be used in future HCM research. Blockade of S100A4 may have therapeutic potential in HCM by attenuating cardiac fibrosis. PMID: 26781584
    23. The experimental and in silico results suggest two divergent evolutionary pathways: NMIIA and NMIIB evolved to possess S100A4-dependent and -independent regulations, respectively. PMID: 27029887
    24. Results found that CNE-1 nasopharyngeal carcinoma cell (NPC) line expressing S100A4 showed significantly increased invasion ability in vitro and in vivo. Also, it seems that aberrant DNA hypomethylation of its promotor region might lead to the metastasis progression in EBV-associated NPC. PMID: 26292668
    25. Oct-1 modifies S100A4 exchange between intra- and extracellular compartments in Namalwa cells and increases their sensitivity to glucocorticoids. PMID: 27096393
    26. ChIP studies showed that the S100A4 protein binds to the GDF15 promoter, implicating S100A4 in GDF15 regulation at the transcriptional level. PMID: 27278086
    27. Cell invasion (matrigel) was reduced only in the Hs578T cells (p < 0.01). Silencing decreased the expression of the prometastatic molecules S100A4 and TGFbetaR2 in both cell lines and CD44 in Hs578T cells .We conclude that ECM1 is a key player in the metastatic process and regulates the actin cytoskeletal architecture of aggressive breast cancer cells at least in part via alterations in S100A4 and Rho A. PMID: 27770373
    28. Aberrant S100A4 expression may predictendometrial cancer (EC) progression and play an important role in regulating EC cell invasion through Epithelial-mesenchymal transition (EMT) regulation. PMID: 27109209
    29. S100A4 could promote the invasion ability of breast cancer cells via EMT, more importantly, it could participate in EMT via regulating MMP2 in breast cancer. PMID: 26409452
    30. our results demonstrate not only that high expression of S100A4 contributes to an aggressive phenotype of EC, but also that its elevated expression is closely related to the MELF histopathological pattern. PMID: 27348205
    31. S100A4, A8, and A9 proteins represent promising marker genes to evaluate the risk potential of suspicious oral lesions in molecular pathology. PMID: 27294692
    32. Increased S100A4 expression is associated with thyroid papillary carcinoma. PMID: 26715280
    33. Comparative analysis of various tumor lines showed no clear correlation between the expression level of Mts1/S100A4 and the content of Oct-1. However, at stable transfection of tumor cells with Oct-1A, Oct-1L, and Oct-1X isoforms we detected an elevated level of Oct-1, which stimulated Mts1/S100A4 secretion. PMID: 27193714
    34. We studied the expression of S100A4 and the total PHF10 protein in some human cancer cell lines. We have found that, in the cell lines studied, the PHF10 expression is correlated with the S100A4 expression. PMID: 27193724
    35. These results support the hypothesis that S100A4 contributes significantly to growth and metastasis, and that down-regulation of S100A4 expression decreases the metastatic potential of ATC cells. PMID: 27802204
    36. Interaction of extracellular S100A4 with RAGE prompts prometastatic activation of melanoma cells. PMID: 26928771
    37. Study shows that negative expression of S100A4 was significantly associated with higher 3-year OS rate and N0 stage for lymph node metastasis in pancreatic neoplasm tumor which suggest that S100A4 expression represents a potential marker for lymph node metastasis and a potential unfavorable factor for prognosis of patients with this disease. PMID: 26903691
    38. S100A4 mRNA level is an important molecular biomarker for prediction of chemosensitivity to gemcitabine in unresectable pancreatic cancer. PMID: 26722531
    39. Tag7-Mts1 complex causes chemotactic migration of lymphocytes, with NK cells being a preferred target. PMID: 26654597
    40. our data suggest a novel mechanism for mutant p53V143A accumulation and add a new facet to the role of S100A4 in cancer PMID: 26497012
    41. S100A4 levels are elevated in human periodontal ligament cells in response to IL-1beta. PMID: 26499072
    42. Enhanced S100A4 expression was observed in remodeled intrapulmonary arteries of COPD patients. PMID: 26483185
    43. Downregulation of AKT3 increases migration and metastasis in triple negative breast cancer cells by upregulating S100A4. PMID: 26741489
    44. vimentin and FSP1 have roles in gingival fibrosis PMID: 25826487
    45. Through selective knockout or inhibition of the network components, we show that the interaction between epidermal growth factor receptor (EGFR) and S100A4 modulates the sensitivity of angiogenesis development to matrix metalloproteinases (MMPs) activity PMID: 26057862
    46. The results show that S100A4 enhances the activity of matrix metalloproteinases (MMPs), causing higher cell dissociation. Moreover, it leads to an increased stability of the pathological state. PMID: 26118552
    47. EZH2 mediates the regulation of E-cadherin expression by S100A4 and controls the proliferation and migration of gastric cancer cells. PMID: 26897964
    48. Proximity ligation assay demonstrate proximity between S100A4 and cyclin B1 in vitro, while confocal microscopy showed S100A4 to localize to areas corresponding to centrosomes in mitotic cells prior to chromosome segregation. PMID: 26349943
    49. Elevated S100A4 in plasma is a novel biomarker for early detection of acute myocardial infarction. PMID: 26166646
    50. PTCSC3 downregulates S100A4, leading to a reduction in cell motility and invasiveness. We propose that PTCSC3 impacts papillary thyroid cancer predisposition and carcinogenesis through the S100A4 pathway. PMID: 26274343

    显示更多

    收起更多

  • 亚细胞定位：
    Secreted. Nucleus. Cytoplasm.
  • 蛋白家族：
    S-100 family
  • 组织特异性：
    Ubiquitously expressed.
  • 数据库链接：

    HGNC: 10494

    OMIM: 114210

    KEGG: hsa:6275

    STRING: 9606.ENSP00000346294

    UniGene: Hs.654444