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  4. Human Protein Wnt-2b(WNT2B) ELISA kit

Human Protein Wnt-2b(WNT2B) ELISA kit

  • 中文名称：
    人Wnt-2b蛋白(WNT2B)酶联免疫试剂盒
  • 货号：
    CSB-EL026134HU
  • 规格：
    96T/48T
  • 价格：
    ￥3600/￥2500
  • 其他：

产品详情

  • 别名：
    WNT2B; WNT13; Protein Wnt-2b; Protein Wnt-13
  • 缩写：
  • Uniprot No.：
  • 种属：
    Homo sapiens (Human)
  • 样本类型：
    serum, plasma, tissue homogenates, cell lysates
  • 检测范围：
    62.5 pg/mL-4000 pg/mL
  • 灵敏度：
    15.6 pg/mL
  • 反应时间：
    1-5h
  • 样本体积：
    50-100ul
  • 检测波长：
    450 nm
  • 研究领域：
    Stem Cells
  • 测定原理：
    quantitative
  • 测定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%        
    Three samples of known concentration were tested twenty times on one plate to assess.    
    Inter-assay Precision (Precision between assays): CV%<10%        
    Three samples of known concentration were tested in twenty assays to assess.      
                   
  • 线性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of human WNT2B in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.  
    　 Sample Serum(n=4)    
    1:1 Average % 87    
    Range % 83-94    
    1:2 Average % 98    
    Range % 94-101    
    1:4 Average % 102    
    Range % 98-105    
    1:8 Average % 95    
    Range % 90-101    
  • 回收率：
    The recovery of human WNT2B spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.  
     
    Sample Type Average % Recovery Range    
    Serum (n=5) 91 85-105    
    EDTA plasma (n=4) 104 88-109    
                   
                   
  • 标准曲线：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.  
     
    pg/ml OD1 OD2 Average Corrected    
    4000 2.513 2.355 2.434 2.285    
    2000 2.012 1.980 1.996 1.847    
    1000 1.220 1.222 1.221 1.072    
    500 0.725 0.721 0.723 0.574    
    250 0.417 0.421 0.419 0.270    
    125 0.298 0.282 0.290 0.141    
    62.5 0.227 0.224 0.226 0.077    
    0 0.148 0.150 0.149 　    
  • 数据处理：
  • 货期：
    3-5 working days

产品评价

靶点详情

  • 功能：
    Ligand for members of the frizzled family of seven transmembrane receptors. Functions in the canonical Wnt/beta-catenin signaling pathway. Plays a redundant role in embryonic lung development.
  • 基因功能参考文献：
    1. Wnt2B co-operates with Frizzled7 to mediate mesenchymal to epithelial transition in colorectal cancer. PMID: 28560804
    2. Altered WNT2B expression in bladder wall fibroblasts alters expression of the apoptosis inductor TNFSF10. PMID: 29131138
    3. we identified two independent SNPs (i.e., WNT2B rs1175649 G>T and BTRC rs61873997 G>A) that showed a predictive role in CM-specific survival, with an effect-allele-attributed hazards ratio (adjusted hazards ratio) of 1.99 (95% confidence interval = 1.41-2.81, P = 8.10 x 10(-5)) and 0.61 (0.46-0.80, 3.12x10(-4)), respectively. PMID: 28499756
    4. A549-Luc-positive cells showed cytotoxicity following exposure to the Ad-shWnt2B vector. The percentage of viable cells was significantly lower in A549-Luc cells treated with Ad-shWnt2B than with Ad-scramble (p<0.01 versus control or Ad-scramble, respectively). PMID: 27793913
    5. ACSL5 mediates antiproliferative activities via Wnt2B palmitoylation with diminished Wnt activity. The molecular pathway is probably relevant for intestinal homeostasis, overwhelmed by other pathways in carcinogenesis. PMID: 25356045
    6. these results indicate that the high-expression levels of Gli1 and Wnt2B might play a pivotal role during tumorigenesis of pancreatic cancer PMID: 25120849
    7. Results showed that miR-185-3p contributs to radioresistance of nasopharyngeal carcinoma via modulation of WNT2B expression. PMID: 25297925
    8. secretion of WNT2B and WNT9B and stabilization of beta-catenin (CTNNB1) upon virus infection negatively regulate expression of representative inducible genes IFNB1, IFIT1 and TNF in a CTNNB1-dependent effector mechanism PMID: 23785285
    9. High Wnt2B overexpression is associated with ovarian cancer metastasis and drug resistance. PMID: 22635028
    10. This is the first report on differential regulation of WNT2 and WNT2B mRNAs in human cancer cell lines. PMID: 11712082
    11. Wnt-2b mRNA is expressed in human ovarian cancer cells PMID: 12072409
    12. REVIEW: Anti-WNT2B monoclonal antibodies, WNT2B RNAi compounds, or small molecule WNT2B inhibitors could be developed as novel therapeutic agents for gastric cancer and esophageal cancer in the field of clinical oncology. PMID: 16273293
    13. in addition to alternative promoters and RNA splicing, an alternative translation start in Wnt13B and Wnt13C mRNAs increases the complexity of both human wnt13 expression and functions PMID: 16407296
    14. Heplipin induced KG-1 cell apoptosis is related with Wntl3 and ATPase3. PMID: 17649721
    15. Hedgehog signals and bHLH transcription factors are involved in WNT2B upregulation, which is counteracted by BMP, WNT and Notch signals. PMID: 19360354

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  • 亚细胞定位：
    Secreted, extracellular space, extracellular matrix. Secreted.
  • 蛋白家族：
    Wnt family
  • 组织特异性：
    Isoform 1 is expressed in adult heart, brain, placenta, lung, prostate, testis, ovary, small intestine and colon. In the adult brain, it is mainly found in the caudate nucleus, subthalamic nucleus and thalamus. Also detected in fetal brain, lung and kidne
  • 数据库链接：

    HGNC: 12781

    OMIM: 601968

    KEGG: hsa:7482

    STRING: 9606.ENSP00000358698

    UniGene: Hs.258575