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  4. Human Protein dopey-2(DOPEY2) ELISA kit

Human Protein dopey-2(DOPEY2) ELISA kit

  • 中文名称：
    人dopey-2蛋白(DOPEY2)酶联免疫试剂盒
  • 货号：
    CSB-EL007119HU
  • 规格：
    96T/48T
  • 价格：
    ￥3600/￥2500
  • 其他：

产品详情

  • 产品描述：

    This Human DOPEY2 ELISA Kit was designed for the quantitative measurement of Human DOPEY2 protein in serum, plasma, tissue homogenates, cell lysates. It is a Sandwich ELISA kit, its detection range is 32 pg/mL-1200 pg/mL and the sensitivity is 8 pg/mL.

  • 别名：
    DOP1B ELISA Kit; C21orf5 ELISA Kit; DOPEY2 ELISA Kit; KIAA0933Protein dopey-2 ELISA Kit
  • 缩写：
    DOPEY2
  • Uniprot No.：
  • 种属：
    Homo sapiens (Human)
  • 样本类型：
    serum, plasma, tissue homogenates, cell lysates
  • 检测范围：
    32 pg/mL-1200 pg/mL
  • 灵敏度：
    8 pg/mL
  • 反应时间：
    1-5h
  • 样本体积：
    50-100ul
  • 检测波长：
    450 nm
  • 研究领域：
    Signal Transduction
  • 测定原理：
    quantitative
  • 测定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<15%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<15%
    Three samples of known concentration were tested in twenty assays to assess.
  • 线性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of human DOPEY2 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    SampleSerum(n=4)
    1:1Average %91
    Range %86-97
    1:2Average %97
    Range %92-103
    1:4Average %85
    Range %81-89
    1:8Average %89
    Range %83-95
  • 回收率：
    The recovery of human DOPEY2 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample TypeAverage % RecoveryRange
    Serum (n=5) 104102-106
    EDTA plasma (n=4)9390-96
  • 标准曲线：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    pg/mlOD1OD2AverageCorrected
    12002.313 2.451 2.382 2.335
    6401.754 1.802 1.778 1.731
    3201.012 1.032 1.022 0.975
    800.345 0.355 0.350 0.303
    320.173 0.174 0.174 0.127
    00.045 0.048 0.047
  • 数据处理：
  • 货期：
    3-5 working days

产品评价

靶点详情

  • 功能：
    May play a role in regulating membrane trafficking of cargo proteins. Together with ATP9A and MON2, regulates SNX3 retromer-mediated endosomal sorting of WLS away from lysosomal degradation.
  • 基因功能参考文献：
    1. The differentially expressed C21orf5 gene in the medial temporal-lobe system could play a role in Down syndrome. PMID: 12767918
    2. C21orf5 selective expression in the key brain structures for learning and memory suggests that C21orf5 overexpression could participate in mental retardation pathogenesis in Down syndrome patients. PMID: 16276086
    3. C21orf5, could play a role in brain morphogenesis and, when overexpressed, it could participate in neurological features and mental retardation observed in DS patients. PMID: 16303751
    4. Results quantify and statistically analyze, for the first time, DOPEY2 expression variations in different regions of the Down syndrome human fetal brains and to compare them to corresponding normal brains. PMID: 19460634
  • 亚细胞定位：
    Early endosome membrane. Golgi apparatus membrane; Peripheral membrane protein.
  • 蛋白家族：
    Dopey family
  • 组织特异性：
    Ubiquitously expressed. Overexpressed in lymphoblasts from Down syndrome patients.
  • 数据库链接：

    HGNC: 1291

    OMIM: 604803

    KEGG: hsa:9980

    STRING: 9606.ENSP00000382104

    UniGene: Hs.204575