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  4. Human Protein kinase C-binding protein NELL1(NELL1) ELISA kit

Human Protein kinase C-binding protein NELL1(NELL1) ELISA kit

  • 中文名称：
    人蛋白激酶C结合蛋白NELL1(NELL1)酶联免疫试剂盒
  • 货号：
    CSB-EL015709HU
  • 规格：
    96T/48T
  • 价格：
    ￥3600/￥2500
  • 其他：

产品详情

  • 产品描述：

    This Human NELL1 ELISA Kit was designed for the quantitative measurement of Human NELL1 protein in serum, plasma, cell culture supernates, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 31.25 pg/mL-2000 pg/mL and the sensitivity is 7.81 pg/mL.

  • 别名：
    FLJ45906 ELISA Kit; IDH3GL ELISA Kit; NEL like 1 (chicken) ELISA Kit; NEL like protein 1 ELISA Kit; Nel related protein 1 ELISA Kit; NEL-like 1 (chicken) ELISA Kit; NEL-like protein 1 ELISA Kit; Nel-related protein 1 ELISA Kit; NELL 1 protein short isoform ELISA Kit; Nell1 ELISA Kit; NELL1_HUMAN ELISA Kit; Neural epidermal growth factor like 1 ELISA Kit; NRP1 ELISA Kit; Protein kinase C binding protein NELL1 ELISA Kit; Protein kinase C-binding protein NELL1 ELISA Kit
  • 缩写：
    NELL1
  • Uniprot No.：
  • 种属：
    Homo sapiens (Human)
  • 样本类型：
    serum, plasma, cell culture supernates, tissue homogenates
  • 检测范围：
    31.25 pg/mL-2000 pg/mL
  • 灵敏度：
    7.81 pg/mL
  • 反应时间：
    1-5h
  • 样本体积：
    50-100ul
  • 检测波长：
    450 nm
  • 研究领域：
    Neuroscience
  • 测定原理：
    quantitative
  • 测定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%      
    Three samples of known concentration were tested twenty times on one plate to assess.  
    Inter-assay Precision (Precision between assays): CV%<10%      
    Three samples of known concentration were tested in twenty assays to assess.    
                 
  • 线性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of human NELL1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
      Sample Serum(n=4)  
    1:5 Average % 91  
    Range % 86-95  
    1:10 Average % 102  
    Range % 97-107  
    1:20 Average % 91  
    Range % 85-97  
    1:40 Average % 97  
    Range % 91-103  
  • 回收率：
    The recovery of human NELL1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample Type Average % Recovery Range  
    Serum (n=5) 95 89-98  
    EDTA plasma (n=4) 92 88-97  
                 
                 
  • 标准曲线：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    pg/ml OD1 OD2 Average Corrected  
    2000 2.907 2.987 2.947 2.768  
    1000 2.260 2.208 2.234 2.055  
    500 1.428 1.435 1.432 1.253  
    250 0.841 0.865 0.853 0.674  
    125 0.538 0.568 0.553 0.374  
    62.5 0.398 0.377 0.388 0.209  
    31.25 0.298 0.271 0.285 0.106  
    0 0.183 0.175 0.179    
  • 数据处理：
  • 货期：
    3-5 working days

产品评价

靶点详情

  • 功能：
    Plays a role in the control of cell growth and differentiation. Promotes osteoblast cell differentiation and terminal mineralization.
  • 基因功能参考文献：
    1. Results show that NELL1 methylation level was 6.8% higher in renal cell cancer (RCC) tissues associated with advanced disease of distant metastases, and shorter RFS. Analyses suggested that NELL1 DNA methylation is a promising candidate prognostic epigenetic biomarker for more detailed analyses of RCC disease. PMID: 30272321
    2. This work indicates that NELL-1, HMGB1, and CCN2 might enhance bone defect healing via the recruitment of endogenous cells and induction of vascularization and act via different processes than BMP2. PMID: 28463604
    3. NELL1 variant is associated with tobacco- and HPV-mediated oral oncogenesis. PMID: 30091681
    4. Results demonstrate that NELL1 is differentially expressed in fusion-positive alveolar rhabdomyosarcoma (ARMS) and in embryonal rhabdomyosarcoma (ERMS) samples, and they show that this transcriptional difference partially depends on genomic DNA methylation. The study also found that high NELL1 levels are correlated with negative RMS prognostic factors and with poor tumor outcomes. PMID: 28380437
    5. lipoaspirate-derived hPSCs present a novel and abundant cell source of MSCs for cartilage regeneration, and the combinatorial application of NELL-1, TGF-beta3, and BMP-6 with hPSCs may remarkably enhance and accelerate cartilage repair. PMID: 26700847
    6. establish the feasibility of combining NELL-1 with BMP2 to improve clinical bone regeneration and provide mechanistic insight into canonical Wnt pathway activity during NELL-1 and BMP2 osteogenesis PMID: 26772960
    7. Data suggest that TSPN (N-terminal thrombospondin-1-like) domain of NELL1 exhibits major heparin-binding sites which may be involved in interaction of NELL1 with cell surface heparan sulfate proteoglycans. PMID: 26627376
    8. NELL-1 signaling activates Wnt/beta-catenin signaling. PMID: 26082355
    9. We also detected lower relative expression of Nell-1 by real-time PCR. Furthermore, immunohistochemical analyses revealed that Nell-1 staining was less intense in cancer tissue relative to normal tissue and that the tumor cells had spread to the muscle PMID: 26090379
    10. CpG islands in the NELL1 and NELL2 promoters are hypermethylated in renal cell carcinoma.NELL1 and NELL2 protein expression is downregulated in clear cell renal carcinoma. PMID: 25726761
    11. NELL-1 demonstrates diffuse and reliable expression in benign but not malignant bone-forming skeletal tumors PMID: 25791475
    12. NELL1 overexpression greatly enhanced the osteogenic differentiation and mineral synthesis of iPSC-MSCs on RGD-grafted CPC scaffold for the first time. PMID: 25220281
    13. Two single-nucleotide polymorphisms of the NELL1 gene may represent a novel mechanism underlying hydrochlorothiazide-induced adverse metabolic effects (meta-analysis). PMID: 23400010
    14. NELL1 is able to promote the osteogenic differentiation of periodontal ligament stem cells, which may be related to the downregulation of Msx2 expression. PMID: 22767336
    15. Activation of the JNK pathway is necessary to mediate terminal osteogenic different-iation of Saos-2 osteosarcoma cells by rhNELL-1. PMID: 22797704
    16. Data indicate that LvNELL1 infection promoted the osteogenic differentiation of hADSCs, and the effect was comparable with that of LvBMP2. PMID: 23017834
    17. SHH and NELL-1 directed signaling produced additive effects on the pro-osteogenic and antiadipogenic differentiation of adipose derived stem cells. PMID: 22264144
    18. Prediction of radiographic severity in Ankolysing Spondylitis based on clinical variables can be significantly improved by including SNPs at ADRB1 (rs1801253), NELL1 (rs8176785) and MHC (rs1634747, rs9270986, rs7451962 and rs241453) genes. PMID: 22495925
    19. These results identify one potential mechanism of action for rhNELL-1 induced osteogenesis and highlight a fundamental difference between NELL-1 and BMP-2 signaling. PMID: 22580275
    20. NELL-1 may produce functional cartilage with properties similar to native cartilage. PMID: 21902605
    21. Results suggest that Osterix is a direct transcriptional regulator with repressive effect on NELL-1 gene expression, contributing to a delicate balance of regulatory effects on NELL-1 transcription with Runx2. PMID: 21931789
    22. Nell-1 is a candidate growth factor able to induce pericyte osteogenic differentiation. PMID: 21615216
    23. the effects of NELL-1 on osteoblastic differentiation and proliferation are partly through binding to APR3 PMID: 21723284
    24. The osteogenic effects of NELL1 on femoral distraction osteogenesis, was investigated. PMID: 20959151
    25. a potent growth factor that is highly specific to the osteochondral lineage, and has demonstrated robust induction of bone in multiple in vivo models [review] PMID: 20647499
    26. Data indicate that NELL1 is a ubiquitous inflammatory bowel disease susceptibility locus. PMID: 17684544
    27. Runx2 directly binds to the osteoblast specific binding elements 2 elements and transactivates the human NELL-1 promoter. PMID: 17042739
    28. Promoter hypermethylation of NELL1 is a common, tissue-specific event in human EAC, occurs early during Barrett's-associated esophageal neoplastic progression, and is a potential biomarker of poor prognosis in early-stage EAC PMID: 17452981
    29. findings suggest that upon binding to a specific receptor NELL1 transduces an osteogenic signal through activation of certain Tyr-kinases associated with the Ras-MAPK cascade, and finally leads to the osteogenic differentiation PMID: 18082140
    30. Clinical trial and genome-wide association study of gene-disease association, gene-environment interaction, and pharmacogenomic / toxicogenomic. (HuGE Navigator) PMID: 18519826
    31. Transgenic mice overexpressing Nell-1 develop craniosynostosis. PMID: 12235118

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  • 亚细胞定位：
    Cytoplasm. Nucleus envelope. Secreted. Note=Colocalizes with ATRAID on the nuclear envelope and the perinuclear region.
  • 数据库链接：

    HGNC: 7750

    OMIM: 602319

    KEGG: hsa:4745

    STRING: 9606.ENSP00000349654

    UniGene: Hs.657172