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Human SPARC-related modular calcium-binding protein 1(SMOC1) ELISA kit

  • 中文名称:
    人SPARC相关模块化钙结合蛋白1(SMOC1)酶联免疫试剂盒
  • 货号:
    CSB-EL021842HU
  • 规格:
    96T/48T
  • 价格:
    ¥3600/¥2500
  • 其他:

产品详情

  • 产品描述:

    This Human SMOC1 ELISA Kit was designed for the quantitative measurement of Human SMOC1 protein in serum, plasma, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 78 pg/mL-5000 pg/mL and the sensitivity is 19.5 pg/mL.

  • 别名:
    Secreted modular calcium-binding protein 1 ELISA Kit; SMOC-1 ELISA Kit; SMOC1 ELISA Kit; SMOC1_HUMAN ELISA Kit; SPARC-related modular calcium-binding protein 1 ELISA Kit
  • 缩写:
  • Uniprot No.:
  • 种属:
    Homo sapiens (Human)
  • 样本类型:
    serum, plasma, tissue homogenates
  • 检测范围:
    78 pg/mL-5000 pg/mL
  • 灵敏度:
    19.5 pg/mL
  • 反应时间:
    1-5h
  • 样本体积:
    50-100ul
  • 检测波长:
    450 nm
  • 研究领域:
    Others
  • 测定原理:
    quantitative
  • 测定方法:
    Sandwich
  • 精密度:
    Intra-assay Precision (Precision within an assay): CV%<8%      
    Three samples of known concentration were tested twenty times on one plate to assess.  
    Inter-assay Precision (Precision between assays): CV%<10%      
    Three samples of known concentration were tested in twenty assays to assess.    
                 
  • 线性度:
    To assess the linearity of the assay, samples were spiked with high concentrations of human SMOC1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.

      Sample Serum(n=4)
    1:1 Average % 96
    Range % 92-100
    1:2 Average % 103
    Range % 99-107
    1:4 Average % 90
    Range % 85-98
    1:8 Average % 92
    Range % 88-96
  • 回收率:
    The recovery of human SMOC1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample Type Average % Recovery Range  
    Serum (n=5) 95 90-101  
    EDTA plasma (n=4) 93 89-97  
  • 标准曲线:
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

    pg/ml OD1 OD2 Average Corrected
    5000 2.518 2.625 2.572 2.439
    2500 2.007 2.149 2.078 1.945
    1250 1.319 1.384 1.352 1.219
    625 0.675 0.629 0.652 0.519
    312 0.421 0.447 0.434 0.301
    156 0.263 0.271 0.267 0.134
    78 0.202 0.211 0.207 0.074
    0 0.130 0.135 0.133  
  • 数据处理:
  • 货期:
    3-5 working days

引用文献

产品评价

靶点详情

  • 功能:
    Plays essential roles in both eye and limb development. Probable regulator of osteoblast differentiation.
  • 基因功能参考文献:
    1. Missense mutation in exon 3 of SMOC1 segregated with the Waardenburg anophthalmia syndrome in the Iranian family. PMID: 28807869
    2. This is the first report of Waardenburg anophthalmia syndrome (WAS) caused by a SMOC1 variant in a Pakistani population. The mutation identified in the present investigation extends the body of evidence implicating the gene SMOC-1 in causing WAS. PMID: 28085523
    3. SMOC binds to Pro-EGF, but does not induce Erk phosphorylation via the EGFR. PMID: 27101391
    4. IL-17A but not IL-22 suppresses the replication of hepatitis B virus by inducing the expression of MxA and OAS. PMID: 23274784
    5. SMOC1 provides a link between prenatal hormone exposure and digit ratio. PMID: 23263445
    6. Homozygosity mapping and subsequent targeted mutation analysis of a locus on 14q24.2 identified homozygous mutations in SMOC1 (SPARC-related modular calcium binding 1) in eight unrelated families with Waardenburg Anophthalmia syndrome. PMID: 21750680
    7. The present study thus identified SPARC related modular calcium binding 1 as a new cancer-associated protein capable of interacting with tenascin-C in vitro PMID: 21349332
    8. these findings indicate that SMOC1/Smoc1 is essential for ocular and limb development in both humans and mice. PMID: 21194678
    9. Waardenburg anophthalmia syndrome is genetically heterogeneous; a second was locus found on chromosome 14, and mutations in SMOC1 were shown also cause this syndrome. PMID: 21194680
    10. Analyzed the secretory protein profiles of BMSCs grown in osteogenic medium (OSM) and identified SPARC-related modular calcium-binding protein 1 (SMOC1), a member of the SPARC family, as a regulator of osteoblast differentiation of BMSCs. PMID: 20359165
    11. isolation of the novel gene SMOC-1 encoding a secreted modular protein containing an EF-hand calcium-binding domain; localization within basement membranes in kidney and skeletal muscle and expression in the zona pellucida surrounding the oocyte PMID: 12130637
    12. SMOC-1 is of physiological interest because it codes a secreted glycoprotein with five domains, each containing regions homologous to those on other proteins that mediate cell-matrix interactions. PMID: 17386346

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  • 相关疾病:
    Ophthalmoacromelic syndrome (OAS)
  • 亚细胞定位:
    Secreted, extracellular space, extracellular matrix, basement membrane. Note=In or around the basement membrane.
  • 组织特异性:
    Widely expressed in many tissues with a strongest signal in ovary. No expression in spleen.
  • 数据库链接:

    HGNC: 20318

    OMIM: 206920

    KEGG: hsa:64093

    STRING: 9606.ENSP00000355110

    UniGene: Hs.497349