Your Good Partner in Biology Research

  1. 首页
  2. 试剂盒
  3. 酶联免疫试剂盒
  4. Human Semaphorin-3G(SEMA3G) ELISA kit

Human Semaphorin-3G(SEMA3G) ELISA kit

  • 中文名称：
    人臂板蛋白-3G(SEMA3G)酶联免疫试剂盒
  • 货号：
    CSB-EL020986HU
  • 规格：
    96T/48T
  • 价格：
    ￥3600/￥2500
  • 其他：

产品详情

  • 产品描述：

    This Human SEMA3G ELISA Kit was designed for the quantitative measurement of Human SEMA3G protein in serum, plasma, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 0.625 ng/mL-40 ng/mL and the sensitivity is 0.156 ng/mL.

  • 别名：
    FLJ00014 ELISA Kit; MGC119473 ELISA Kit; Sem2 ELISA Kit; SEM3G_HUMAN ELISA Kit; Sema domain immunoglobulin domain (Ig) short basic domain secreted (semaphorin) 3G ELISA Kit; Sema3g ELISA Kit; Semaphorin 3G ELISA Kit; Semaphorin sem2 ELISA Kit; Semaphorin-3G ELISA Kit
  • 缩写：
    SEMA3G
  • Uniprot No.：
  • 种属：
    Homo sapiens (Human)
  • 样本类型：
    serum, plasma, tissue homogenates
  • 检测范围：
    0.625 ng/mL-40 ng/mL
  • 灵敏度：
    0.156 ng/mL
  • 反应时间：
    1-5h
  • 样本体积：
    50-100ul
  • 检测波长：
    450 nm
  • 研究领域：
    Immunology
  • 测定原理：
    quantitative
  • 测定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%
    Three samples of known concentration were tested in twenty assays to assess.
  • 线性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of human SEMA3G in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    　 Sample Serum(n=4)
    1:1 Average % 97
    Range % 91-102
    1:2 Average % 101
    Range % 96-105
    1:4 Average % 94
    Range % 87-98
    1:8 Average % 99
    Range % 91-103
  • 回收率：
    The recovery of human SEMA3G spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample Type Average % Recovery Range
    Serum (n=5) 87 82-91
    EDTA plasma (n=4) 97 90-100
  • 标准曲线：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    ng/ml OD1 OD2 Average Corrected
    40 2.005 1.941 1.973 1.824
    20 1.613 1.576 1.595 1.446
    10 1.144 1.109 1.127 0.978
    5 0.746 0.733 0.740 0.591
    2.5 0.544 0.524 0.534 0.385
    1.25 0.383 0.372 0.378 0.229
    0.625 0.275 0.269 0.272 0.123
    0 0.152 0.146 0.149 　
  • 数据处理：
  • 货期：
    3-5 working days

产品评价

靶点详情

  • 功能：
    Has chemorepulsive activities for sympathetic axons. Ligand of NRP2.
  • 基因功能参考文献：
    1. Results indicate that Sema3G protein is secreted by podocytes and protects them from inflammatory kidney diseases and diabetic nephropathy. PMID: 27180624
    2. Results identify Sema3g as one of the downstream effectors of PPAR-gamma, which is centrally involved in regulating endothelial cell migration. PMID: 25335934
    3. overexpressed SEMA3G inhibited the migratory and invasive behavior of U251MG cancer cells PMID: 22562223
    4. Identify Sema3G as a primarily endothelial cell-expressed class 3 semaphorin that controls endothelial and smooth muscle cell functions in autocrine and paracrine manners. PMID: 20947821
    5. In gliomas, SEMA3B, SEMA3G and NRP2 expressions were related to prolonged survival PMID: 18781179
    6. Sema3G inhibits tumor progression from MDA-MB-435 but not from MDA-MB-231 cancer cells. It inhibits tumor angiogenesis in MDA-MB-231 cells. PMID: 18818766

    显示更多

    收起更多

  • 亚细胞定位：
    Secreted.
  • 蛋白家族：
    Semaphorin family
  • 数据库链接：

    HGNC: 30400

    KEGG: hsa:56920

    STRING: 9606.ENSP00000231721

    UniGene: Hs.59729