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  4. Human Small proline-rich protein 2B(SPRR2B) ELISA kit

Human Small proline-rich protein 2B(SPRR2B) ELISA kit

  • 中文名称：
    人小富脯氨酸蛋白2B(SPRR2B)酶联免疫试剂盒
  • 货号：
    CSB-EL022613HU
  • 规格：
    96T/48T
  • 价格：
    ￥3600/￥2500
  • 其他：

产品详情

  • 产品描述：

    This Human SPRR2B ELISA Kit was designed for the quantitative measurement of Human SPRR2B protein in serum, plasma, tissue homogenates, cell lysates. It is a Sandwich ELISA kit, its detection range is 31.25 pg/mL-2000 pg/mL and the sensitivity is 7.81 pg/mL.

  • 别名：
    SPRR2B ELISA Kit; Small proline-rich protein 2B ELISA Kit; SPR-2B ELISA Kit
  • 缩写：
    SPRR2B
  • Uniprot No.：
  • 种属：
    Homo sapiens (Human)
  • 样本类型：
    serum, plasma, tissue homogenates, cell lysates
  • 检测范围：
    31.25 pg/mL-2000 pg/mL
  • 灵敏度：
    7.81 pg/mL
  • 反应时间：
    1-5h
  • 样本体积：
    50-100ul
  • 检测波长：
    450 nm
  • 研究领域：
    Others
  • 测定原理：
    quantitative
  • 测定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%
    Three samples of known concentration were tested in twenty assays to assess.
  • 线性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of human SPRR2B in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    SampleSerum(n=4)
    1:1Average %90
    Range %87-96
    1:2Average %103
    Range %99-105
    1:4Average %93
    Range %87-97
    1:8Average %91
    Range %88-96
  • 回收率：
    The recovery of human SPRR2B spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample TypeAverage % RecoveryRange
    Serum (n=5) 9692-101
    EDTA plasma (n=4)9086-94
  • 标准曲线：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    pg/mlOD1OD2AverageCorrected
    20002.221 2.161 2.191 2.085
    10001.864 1.781 1.823 1.717
    5001.425 1.546 1.486 1.380
    2501.212 1.157 1.185 1.079
    1250.746 0.795 0.771 0.665
    62.50.357 0.350 0.354 0.248
    31.250.236 0.250 0.243 0.137
    00.104 0.108 0.106
  • 数据处理：
  • 货期：
    3-5 working days

产品评价

靶点详情

  • 功能：
    Cross-linked envelope protein of keratinocytes. It is a keratinocyte protein that first appears in the cell cytosol, but ultimately becomes cross-linked to membrane proteins by transglutaminase. All that results in the formation of an insoluble envelope beneath the plasma membrane.
  • 基因功能参考文献：
    1. Knockdown of the Sprr2b gene or inhibition of SPRR2B phosphorylation attenuates USP7/MDM2 binding and p53 degradation, leading to fibroblasts cell cycle arrest. PMID: 29581288
    2. An SNP in SPRR2B was predictive of asthma among white children with eczema from 2 independent populations. PMID: 22374195
    3. We showed that just four genes, G3BP2, SCARB2, CSNK1A1 and SPRR2B, can classify patients as presence of lymph node metastasis negative or positive, with 80.0% accuracy. PMID: 21985131
    4. SPR2B transcripts and protein were moderately increased in the human papillomaviurs 11- and 59-infected tissues. PMID: 14748073
  • 亚细胞定位：
    Cytoplasm.
  • 蛋白家族：
    Cornifin (SPRR) family
  • 组织特异性：
    Suprabasal layers of squamous-differentiated tissues such as epidermis, esophagus, tongue and trachea.
  • 数据库链接：

    HGNC: 11262

    OMIM: 182268

    KEGG: hsa:6701

    STRING: 9606.ENSP00000340703

    UniGene: Hs.568239