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  4. Human ATP-binding cassette sub-family C member 5 (ABCC5) ELISA Kit

Human ATP-binding cassette sub-family C member 5 (ABCC5) ELISA Kit

  • 中文名称：
    人ATP-结合盒式亚家族C成员5 (ABCC5)酶联免疫试剂盒
  • 货号：
    CSB-E13725h
  • 规格：
    96T/48T
  • 价格：
    ￥3600/￥2500
  • 其他：

产品详情

  • 产品描述：

    This Human ABCC5 ELISA Kit was designed for the quantitative measurement of Human ABCC5 protein in serum, plasma, tissue homogenates, cell lysates. It is a Sandwich ELISA kit, its detection range is 0.312 pmol/mL-20 pmol/mL and the sensitivity is 0.078 pmol/mL.

  • 别名：
    ABC 33 ELISA Kit; ABC33 ELISA Kit; ABCC 5 ELISA Kit; Abcc5 ELISA Kit; ATP binding cassette sub family C (CFTR/MRP) member 5 ELISA Kit; ATP binding cassette sub family C member 5 ELISA Kit; ATP-binding cassette sub-family C member 5 ELISA Kit; Canalicular multispecific organic anion transporter C ELISA Kit; DKFZp686C1782 ELISA Kit; EST277145 ELISA Kit; MOAT C ELISA Kit; MOAT-C ELISA Kit; MOATC ELISA Kit; MRP 5 ELISA Kit; MRP5_HUMAN ELISA Kit; Multi specific organic anion tranporter C ELISA Kit; Multi-specific organic anion transporter C ELISA Kit; Multidrug resistance associated protein 5 ELISA Kit; Multidrug resistance-associated protein 5 ELISA Kit; pABC 11 ELISA Kit; pABC11 ELISA Kit; SMRP ELISA Kit
  • 缩写：
  • Uniprot No.：
  • 种属：
    Homo sapiens (Human)
  • 样本类型：
    serum, plasma, tissue homogenates, cell lysates
  • 检测范围：
    0.312 pmol/ml-20 pmol/ml
  • 灵敏度：
    0.078 pmol/ml
  • 反应时间：
    1-5h
  • 样本体积：
    50-100ul
  • 检测波长：
    450 nm
  • 研究领域：
    Signal Transduction
  • 测定原理：
    quantitative
  • 测定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%      
    Three samples of known concentration were tested twenty times on one plate to assess.  
    Inter-assay Precision (Precision between assays): CV%<10%      
    Three samples of known concentration were tested in twenty assays to assess.    
                 
  • 线性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of human SMRP in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    　SampleSerum(n=4)
    1:1Average %88
    Range %84-94
    1:2Average %96
    Range %91-100
    1:4Average %103
    Range %92-112
    1:8Average %89
    Range %80-98
  • 回收率：
    The recovery of human SMRP spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample Type Average % Recovery Range  
    Serum (n=5) 99 95-105  
    EDTA plasma (n=4) 89 80-98  
                 
                 
  • 标准曲线：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    pmol/ml OD1 OD2 Average Corrected  
    20 2.795 2.603 2.699 2.549  
    10 2.320 2.192 2.256 2.106  
    5 1.564 1.545 1.555 1.405  
    2.5 0.913 0.878 0.896 0.746  
    1.25 0.515 0.501 0.508 0.358  
    0.625 0.289 0.279 0.284 0.134  
    0.312 0.227 0.221 0.224 0.074  
    0 0.151 0.149 0.150 　  
  • 数据处理：
  • 货期：
    3-5 working days

产品评价

靶点详情

  • 功能：
    ATP-dependent transporter of the ATP-binding cassette (ABC) family that actively extrudes physiological compounds, and xenobiotics from cells. Mediates ATP-dependent transport of endogenous metabolites such as cAMP and cGMP, folic acid and N-lactoyl-amino acids (in vitro). Acts also as a general glutamate conjugate and analog transporter that can limit the brain levels of endogenous metabolites, drugs, and toxins. Confers resistance to the antiviral agent PMEA. Able to transport several anticancer drugs including methotrexate, and nucleotide analogs in vitro, however it does with low affinity, thus the exact role of ABCC5 in mediating resistance still needs to be elucidated. Acts as a heme transporter required for the translocation of cytosolic heme to the secretory pathway. May play a role in energy metabolism by regulating the glucagon-like peptide 1 (GLP-1) secretion from enteroendocrine cells.
  • 基因功能参考文献：
    1. ABCC5 polymorphisms may explain partially the interpatient variability in doxorubicin disposition. PMID: 26975227
    2. This study is the first to identify the roles of FOXM1 in drug efflux and paclitaxel resistance by regulating the gene transcription of abcc5, one of the ABC transporters. Small molecular inhibitors of FOXM1 or ABCC5 have the potential to overcome paclitaxel chemoresistance in nasopharyngeal carcinoma (NPC)patients. PMID: 28277541
    3. These findings enrich the allelic spectrum of ABCC5 in PACG. We identified no tagging SNP responsible for the association of the whole region. PMID: 28813580
    4. Survivors of childhood acute lymphoblastic leukemia treated with doxorubicin with the ABCC5 TT-1629 genotype had an average of 8-12% reduction of ventricular ejection and shortening fractions. PMID: 26345518
    5. our work indicated that decreased SLC34A2 expression sensitized BCSCs to doxorubicin via SLC34A2-Bmi1-ABCC5 signaling and shed new light on understanding the mechanism of chemoresistance in BCSCs. This study not only bridges the missing link between stem cell-related transcription factor (Bmi1) and ABC transporter (ABCC5) but also contributes to development of potential therapeutics against breast cancer. PMID: 26546432
    6. Deletions of ABCC5 predict good tumor response to neoadjuvant chemotherapy in breast cancer. PMID: 26799285
    7. genetic association study in population in Mexico: Data suggest SNPs in ABCC5 (3933+313T>C) are not associated with adverse reactions to methotrexate; they protect against myelosuppression in pediatric patients with ALL (acute lymphoblastic leukemia). PMID: 26353179
    8. We identified several genes (FasL, MSH2, ABCC5, CASP3, and CYP3A4)that showed association with PFS in patients with osteosarcoma. These pharmacogenetic risk factors might be useful to predict treatment outcome PMID: 25829401
    9. ABCC5 is a general glutamate conjugate and analog transporter that affects the disposition of endogenous metabolites, toxins, and drugs. PMID: 26515061
    10. The present study investigated the time course and dose dependency of the induction of three efflux proteins, P-gp, MRP1 and MRP5, in response to gemcitabine exposure in Capan-2 pancreatic cancer cell line at transcriptional and translational levels. PMID: 25564970
    11. cCMP is a substrate for MRP5 PMID: 25017019
    12. The blockade of ABC transporter MRP5 could help to improve drug effectiveness, reduce tumour growth and prevent recurrence in glioblastoma multiforme. PMID: 25028266
    13. To identify substrates of orphan transporter ATP-binding cassette subfamily C member 5 (ABCC5), identified a class of metabolites, N-lactoyl-amino acids, and found that a protease, cytosolic nonspecific dipeptidase 2 (CNDP2), catalyzes their formation. PMID: 25964343
    14. ABCC5 transporter is a novel type 2 diabetes susceptibility gene in European and African American populations. PMID: 25117150
    15. ABCC5 functions as a mediator of breast cancer skeletal metastasis. ABCC5 expression in breast cancer cells is important for efficient osteoclast-mediated bone resorption. PMID: 23174366
    16. This locus was associated with an increase in risk of PACG in a separate case-control study of 4,276 primary angle closure glaucoma cases and 18,801 controls (per-allele OR = 1.13 [95% CI: 1.06-1.22], P = 0.00046). PMID: 24603532
    17. Further research is needed to clarify whether MRP5 is indicative of malignant pathological features in meningiomas and whether possible therapeutic implications exist PMID: 23271324
    18. PDE5 is highly expressed and increases ( approximately 130%) during growth whereas ABCC5 exhibited low to moderate expression, with a moderate increase ( approximately 40%) during growth PMID: 22843873
    19. The present results imply that MRP5 index may hold a prognostic role in patients with glioblastoma multiforme PMID: 22278731
    20. Findings show that the gene expression of ATP-dependent efflux transporters MRP1, -3, -4, -5, and p-gp fluctuated during stem cell-derived retinal pigment epithelial cells (hESC-RPE) maturation. PMID: 22272278
    21. This gene-wide tagging study revealed no association between ABCC2, ABCC5 and ABCG2 genetic polymorphisms and multidrug resistance in epilepsy PMID: 21449672
    22. Results support the idea that isolating survivin and MRP5+ CTCs may help in the selection of metastatic colorectal cancer patients resistant to standard 5-FU and L-OHP based chemotherapy, for which alternative regimens may be appropriate. PMID: 20597995
    23. Results identify key membrane transporters OATP2B1, MRP1, MRP4, and MRP5 as modulators of skeletal muscle statin exposure and toxicity. PMID: 19940267
    24. MDR1, MRP2, MRP3 and MRP5 levels are decreased by tetramethylpyrazine in human hepatocellular carcinoma cells PMID: 19956884
    25. Sodium influx is not directly coupled to MRP4/5-mediated cyclic nucleotide efflux, is independent of the status of transporter activity, and determines the baseline membrane potential, which in turns modulates MRP4/5-mediated cyclic nucleotide efflux. PMID: 19903828
    26. MRP4 and MRP5 are low affinity cyclic nucleotide transporters that may at best function as overflow pumps, decreasing steep increases in cGMP levels under conditions where cGMP synthesis is strongly induced and phosphodiesterase activity is limiting PMID: 12637526
    27. MRP1 and MRP5 increase with trophoblast maturation, suggesting a particular role for these proteins in the organ functional developmen PMID: 12646196
    28. the affinity of MRP4 and MRP5 for nucleotide-based substrates is low. PMID: 12695538
    29. Localized in cardiac and cardiovascular myocytes as well as endothelial cells with increased expression in ischemic cardiomyopathy. MRP5-mediated cellular export may represent novel, disease-dependent pathway for cGMP removal from cardiac cells. PMID: 14507663
    30. MRP5 expression depends on gestational age and varies throughout the differentiation process. PMID: 15631998
    31. Upregulation of MRP5 expression is associated with pancreatic carcinoma PMID: 15688370
    32. analysis of linkage disequilibrium at the nucleotide analogue transporter ABCC5 gene locus PMID: 15861033
    33. Plant flavonoids were able to reverse drug resistance in human cells transfected with ABCC5 PMID: 16156793
    34. We have isolated three novel ABCC5 splice variants and characterized their role in the regulation of ABCC5 gene expression. One ABCC5 splice variant produces a truncated ABCC5 protein isoform with thus far unknown functional properties in the retina. PMID: 17521428
    35. cGMP may be a physiological regulator of hyaluronan export at the level of the export MRP5 PMID: 17540771
    36. analysis of the outward facing state of the human P-glycoprotein (ABCB1), and comparison to a model of the human MRP5 (ABCC5) PMID: 17803828
    37. Celecoxib upregulates MRP5 in colon cancer and results in lack of synergy with standard chemotherapy. PMID: 18690847
    38. MRP3, MRP4, and MRP5 are upregulated in 5-fluorouracil-resistant cells, and MRP5 contributes to 5-FU resistance in pancreatic carcinoma cells. PMID: 19077464

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  • 亚细胞定位：
    Basolateral cell membrane; Multi-pass membrane protein. Golgi apparatus lumen. Endosome membrane. Cytoplasmic granule. Apical cell membrane; Multi-pass membrane protein.
  • 蛋白家族：
    ABC transporter superfamily, ABCC family, Conjugate transporter (TC 3.A.1.208) subfamily
  • 组织特异性：
    [Isoform 3]: Predominant isoform in retinal pigment epithelium, bladder, and stomach.; Ubiquitously expressed, but levels in brain and muscle are especially high. All isoforms are equally expressed in retina.
  • 数据库链接：

    HGNC: 56

    OMIM: 605251

    KEGG: hsa:10057

    STRING: 9606.ENSP00000333926

    UniGene: Hs.368563