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  4. Human Stem cell factor/mast cell growth factor,SCF/MGF ELISA kit

Human Stem cell factor/mast cell growth factor,SCF/MGF ELISA kit

  • 中文名称：
    人干细胞因子/肥大细胞生长因子(SCF/MGF)酶联免疫试剂盒
  • 货号：
    CSB-E04718h
  • 规格：
    96T/48T
  • 价格：
    ￥3200/￥2500
  • 其他：

产品详情

  • 产品描述：

    This human SCF ELISA kit employs the quantitative sandwich enzyme immunoassay technique to measure the levels of human SCF in multiple samples, including serum, plasma, or cell culture supernates. It also uses the enzyme-substrate chromogenic reaction to visualize and analyze the analyte levels through the color intensity. The intensity of the colored product is in direct proportion to the SCF levels in the sample and is measured at 450 nm through a microplate reader.

    SCF, also called KITLG or MGF, is of neural and smooth muscle origin and is involved in the regulation of cell proliferation, differentiation, and migration. It is a cytokine that modulates hematopoiesis and other biological processes like mast cell maturation and activation. SCF-c-Kit signaling plays a key role in the maintenance of the hematopoietic stem cells (HSCs) and progenitor cells in the bone marrow. SCF is also critical in regulating the proliferation, differentiation and function of the interstitial cells of Cajal (ICCs), which are closely related to smooth muscle dysfunction.

  • 别名：
    C kit ligand ELISA Kit; C-kit ligand ELISA Kit; Ckit ligand ELISA Kit; DCUA ELISA Kit; DFNA69 ELISA Kit; DKFZp686F2250 ELISA Kit; familial progressive hyperpigmentation 2 ELISA Kit; FPH2 ELISA Kit; FPHH ELISA Kit; KIT ligand ELISA Kit; Kitl ELISA Kit; KITLG ELISA Kit; KL 1 ELISA Kit; KL1 ELISA Kit; Mast cell growth factor ELISA Kit; MGF ELISA Kit; MGF stem cell factor ELISA Kit; SCF ELISA Kit; SCF_HUMAN ELISA Kit; SF ELISA Kit; SHEP7 ELISA Kit; sKITLG ELISA Kit; Soluble KIT ligand ELISA Kit; Steel factor ELISA Kit; steel, mouse, homolog of ELISA Kit; Stem cell factor ELISA Kit; Stem cell factor precursor ELISA Kit
  • 缩写：
  • Uniprot No.：
  • 种属：
    Homo sapiens (Human)
  • 样本类型：
    serum, plasma, cell culture supernates
  • 检测范围：
    0.45 ng/mL-30 ng/mL
  • 灵敏度：
    0.11 ng/mL
  • 反应时间：
    1-5h
  • 样本体积：
    50-100ul
  • 检测波长：
    450 nm
  • 研究领域：
    Immunology
  • 测定原理：
    quantitative
  • 测定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%
    Three samples of known concentration were tested in twenty assays to assess.
  • 线性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of human SCF/MGF in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    SampleSerum(n=4)
    1:1Average %92
    Range %87-96
    1:2Average %86
    Range %83-89
    1:4Average %92
    Range %88-97
    1:8Average %91
    Range %87-94
  • 回收率：
    The recovery of human SCF/MGF spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample TypeAverage % RecoveryRange
    Serum (n=5) 10398-107
    EDTA plasma (n=4)8682-92
  • 标准曲线：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    ng/mlOD1OD2AverageCorrected
    302.624 2.522 2.573 2.461
    151.796 1.897 1.847 1.735
    7.51.281 1.188 1.235 1.123
    3.750.739 0.713 0.726 0.614
    1.80.429 0.404 0.417 0.305
    0.90.277 0.295 0.286 0.174
    0.450.215 0.212 0.214 0.102
    00.111 0.113 0.112
  • 数据处理：
  • 货期：
    3-5 working days

产品评价

靶点详情

  • 功能：
    Ligand for the receptor-type protein-tyrosine kinase KIT. Plays an essential role in the regulation of cell survival and proliferation, hematopoiesis, stem cell maintenance, gametogenesis, mast cell development, migration and function, and in melanogenesis. KITLG/SCF binding can activate several signaling pathways. Promotes phosphorylation of PIK3R1, the regulatory subunit of phosphatidylinositol 3-kinase, and subsequent activation of the kinase AKT1. KITLG/SCF and KIT also transmit signals via GRB2 and activation of RAS, RAF1 and the MAP kinases MAPK1/ERK2 and/or MAPK3/ERK1. KITLG/SCF and KIT promote activation of STAT family members STAT1, STAT3 and STAT5. KITLG/SCF and KIT promote activation of PLCG1, leading to the production of the cellular signaling molecules diacylglycerol and inositol 1,4,5-trisphosphate. KITLG/SCF acts synergistically with other cytokines, probably interleukins.
  • 基因功能参考文献：
    1. s show here that cyclin F, a substrate receptor F-box protein for the SCF (Skp1/Cul1/F-box) family of E3 ubiquitin ligases, is a bona fide AKT substrate. PMID: 28954236
    2. A statistically significant relationship was found between stem cell factor level and oocyte maturation, embryo quality, and clinical pregnancy. PMID: 28285567
    3. PAR2 plays a direct role in melanogenesis by increasing SCF secretion from keratinocytes. PMID: 26909822
    4. We did not find any KITLG mutation in two affected individuals with familial progressive hyperpigmentation and hypopigmentation. PMID: 27859606
    5. results suggest that SCF can accelerate cell homing and the maturation of the pulp-dentin complex in human immature teeth. PMID: 27762658
    6. SCF is a critical regulatory factor for conceptus development and implantation during pregnancy in pigs. PMID: 28551414
    7. results revealed that the Th17-mediated inflammatory environment promotes mast cell accumulation through keratinocyte-derived SCF PMID: 28456630
    8. Bone marrow adipocytes synthesize SCF, promoting hematopoietic stem cell proliferation/regeneration. PMID: 28714970
    9. Hypoxia microenvironment in hepatocellular carcinoma up-regulates HIF2/SCF expression, which in turn promotes angiogenesis and HCC metastasis. PMID: 28153790
    10. Insights into the transmembrane helix associations of Kit ligand have been reported. PMID: 28370370
    11. There were no significant differences in CD334-SCF double-positive FB and CD31-SCF double-positive EC levels between the two periapical tissue groups (P > 0.05). CD14-SCF double-positive MP density was considerably higher in periapical granulomas than in cysts (P < 0.01). PMID: 28290616
    12. A significant association of rs995030 of the KITLG gene with the development of testicular germ cell tumors (p = 0.029 for the allele G, p = 0.0124 for the genotype GG) was revealed. PMID: 28064312
    13. Overexpression of SCF is associated with Lewis lung carcinoma. PMID: 26666817
    14. both SCF and IL-31 play an important role in mediating inflammation and enhancing severity of atopic asthma. PMID: 26853551
    15. women with endometriosis have low levels of endometrial SCF PMID: 26791471
    16. results suggested the existence of E2F1-miR-34c-SCF negative feedback loop which was interrupted by the hyper-methylation of miR-34c promoter in CRC cells and increased cell proliferation. PMID: 26704889
    17. IL-1b, which normally bridges innate and adaptive immunity, induces the production of the major haematopoietic/proleukaemic growth factor SCF through the PI-3K/mTOR pathway and the HIF-1a transcription complex. PMID: 25418470
    18. We identified a new heterozygous missense mutation in two families with hyper- and hypopigmentation disorders (p.Thr34Asn and p.Val37Gly, respectively). Of the six affected individuals examined by us, two had cancer. PMID: 26179221
    19. Na+/H+ antiporter inhibition induces cell death in TF-1 erythroleukemia cells stimulated by the stem cell factor PMID: 26188365
    20. IL-7 and SCF are elevated for a prolonged period after double umbilical cord blood transplantation and persistently high levels of these cytokines may correlate with worse clinical outcomes. PMID: 26177551
    21. Allelic Mutations of KITLG, Encoding KIT Ligand, Cause Asymmetric and Unilateral Hearing Loss and Waardenburg Syndrome Type 2. PMID: 26522471
    22. Foxc1 is essential for progenitor cell development and maintenance of bone marrow niches for these cells upregulating CXCL12 and SCF expression and inhibiting of adipogenic processes in cell progenitors. (Review) PMID: 26049754
    23. Results identified SCF as a direct target of HIF-1 in pancreatic cancer. SCF/c-kit promoted invasion and proliferation of pancreatic cancer cells and may be a new target for the treatment of pancreatic cancer. PMID: 25799412
    24. we found that Kl treatment impairs Nodal mRNA expression and Fgf9-mediated Nanos2 induction, reinforcing the antagonistic effect of these two growth factors on the meiotic fate of male germ cells PMID: 25766327
    25. A functional p53 responsive polymorphism in KITLG, rs4590952, does not affect the risk of breast cancer. PMID: 25220285
    26. High SCF expression is associated with metastasis in non-small cell lung cancers. PMID: 25401222
    27. SCF-mediated gamma-globin gene expression in adult human erythroid cells is associated with KLF1, BCL11A and SOX6 down-regulation. PMID: 25457384
    28. overexpression of Snail in PDAC cells increased SCF levels, and the media conditioned by Snail-expressing PDAC cells promoted mast cell migration. PMID: 24944064
    29. Paraffin-embedded, formalin-fixed tissue from 100 consecutive patients with invasive breast cancer was immunohistochemically stained for Ki-67 and Ki-67/KL1. Ki-67 was manually scored in different regions by 2 observers and automated image analysis PMID: 24897070
    30. KIT-L could be now a direct NOBOX target. PMID: 25514101
    31. Stem cell factor is a novel independent prognostic biomarker for hepatocellular carcinoma after curative resection. PMID: 25086759
    32. Together, these data indicate that stem cell factor1-165 is more thermostable than stem cell factor1-141. PMID: 25061857
    33. High KITLG is associated with growth and invasion in colorectal cancer. PMID: 25213795
    34. These findings suggest that integration of SCF and LTD4 signals may contribute to MC hyperplasia and hyper-reactivity during airway hyper-response and inflammation PMID: 25161061
    35. study reports a modulatory role for antimullerian hormone as an ovarian/follicular autocrine/paracrine factor controlling SCF expression via the cAMP/PKA pathway in granulosa cells PMID: 25241372
    36. Gain-of-function mutations in c-Kit result mastocytosis, gastrointestinal stromal tumors and other hematological neoplasms. (Review) PMID: 24745671
    37. Variants of the KITLG enhancer is associated with blond hair color. PMID: 24880339
    38. Data reveal that SCF disrupts the endothelial adherens junction and enhances vascular leakage, and suggest that anti-SCF/cKit therapy may hold promise as a potential therapy for the treatment of hyperpermeable vascular diseases. PMID: 24790137
    39. Data indicate that insulin-like growth factor-1 (IGF1) stimulated s tem cell factor (Kitl/KITLG) protein and mRNA expression and promoter activity by activating several signaling pathways. PMID: 24116170
    40. Cardiomyocyte-specific overexpression of SCF protects the heart from myocardial ischemia reperfusion injury. PMID: 23680593
    41. Data indicate that stem cell factor (SCF) was expressed in differentiating dental pulp. PMID: 23703692
    42. Seborrheic keratosis cells do not respond to IL-1alpha or TNF-alpha to stimulate production of SCF, whereas a significant stimulation of SCF is elicited by those same cytokines in normal human keratinocytes. PMID: 23662587
    43. Study uncovered a single-nucleotide polymorphism in a functional p53-binding site and establish its influence on the ability of p53 to bind to and regulate transcription of the KITLG gene. The SNP resides in KITLG and associates with one of the largest risks identified among cancer genome-wide association studies. PMID: 24120139
    44. N-linked carbohydrate modifiers affect stem cell factor and endothelin-1-stimulated epidermal pigmentation in human epidermal equivalents PMID: 23273644
    45. RIN3 is a negative regulator of mast cell responses to SCF PMID: 23185384
    46. this is the first report to determine the significant contribution of SCF autoproduction to neoplastic proliferation of mast cells PMID: 23125456
    47. A unique requirement for KIT Ligand in the upregulation of glucocorticoid receptor alpha isoform and optimal erythroblast expansion in cultures that mimic stress erythropoiesis. PMID: 22533504
    48. Stem cell factor was expressed in all tumour subgroups, with the highest in oncocytomas and papillary renal cell carcinomas. Stem cell factor correlated positively with the cytoplasmic expression of KIT. PMID: 23060556
    49. Elevated plasma levels of SCF are associated with inflammatory bowel disease with JCV infection. PMID: 22467521
    50. The KITLG (rs4474514) variants were significantly associated with germ cell tumors only in the adolescent age group. PMID: 22072546

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  • 相关疾病：
    Hyperpigmentation with or without hypopigmentation, familial progressive (FPHH); Deafness, congenital, unilateral or asymmetric (DCUA)
  • 亚细胞定位：
    [Isoform 1]: Cell membrane; Single-pass type I membrane protein.; [Isoform 2]: Cytoplasm. Cytoplasm, cytoskeleton. Cell membrane; Single-pass type I membrane protein. Cell projection, lamellipodium. Cell projection, filopodium.; [Soluble KIT ligand]: Secreted.
  • 蛋白家族：
    SCF family
  • 数据库链接：

    HGNC: 6343

    OMIM: 145250

    KEGG: hsa:4254

    STRING: 9606.ENSP00000228280

    UniGene: Hs.1048