Your Good Partner in Biology Research

  1. 首页
  2. 试剂盒
  3. 酶联免疫试剂盒
  4. Human Thiosulfate sulfurtransferase(TST) ELISA kit

Human Thiosulfate sulfurtransferase(TST) ELISA kit

  • 中文名称：
    人硫代硫酸硫转移酶(TST)酶联免疫试剂盒
  • 货号：
    CSB-EL025197HU
  • 规格：
    96T/48T
  • 价格：
    ￥3600/￥2500
  • 其他：

产品详情

  • 产品描述：

    This Human TST ELISA Kit was designed for the quantitative measurement of Human TST protein in serum, plasma, tissue homogenates, cell lysates. It is a Sandwich ELISA kit, its detection range is 15.6 pg/mL-1000 pg/mL and the sensitivity is 3.9 pg/mL .

  • 别名：
    GlpE ELISA Kit; RDS ELISA Kit; Rhodanese ELISA Kit; Thiosulfate sulfurtransferase ELISA Kit; Thiosulfate sulfurtransferase GlpE ELISA Kit; THTR_HUMAN ELISA Kit; TST ELISA Kit
  • 缩写：
    TST
  • Uniprot No.：
  • 种属：
    Homo sapiens (Human)
  • 样本类型：
    serum, plasma, tissue homogenates, cell lysates
  • 检测范围：
    15.6 pg/mL-1000 pg/mL
  • 灵敏度：
    3.9 pg/mL
  • 反应时间：
    1-5h
  • 样本体积：
    50-100ul
  • 检测波长：
    450 nm
  • 研究领域：
    Metabolism
  • 测定原理：
    quantitative
  • 测定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%
    Three samples of known concentration were tested in twenty assays to assess.
  • 线性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of human TST in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    　SampleSerum(n=4)
    1:1Average %86
    Range %80-92
    1:2Average %95
    Range %91-105
    1:4Average %100
    Range %92-110
    1:8Average %92
    Range %86-98
  • 回收率：
    The recovery of human TST spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample TypeAverage % RecoveryRange
    Serum (n=5) 9389-98
    EDTA plasma (n=4)9590-100
  • 标准曲线：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    pg/mlOD1OD2AverageCorrected
    10002.504 2.511 2.508 2.352
    5002.131 2.052 2.092 1.936
    2501.415 1.333 1.374 1.218
    1250.759 0.744 0.752 0.596
    62.50.541 0.523 0.532 0.376
    31.20.337 0.323 0.330 0.174
    15.60.251 0.244 0.248 0.092
    00.159 0.153 0.156 　
  • 数据处理：
  • 货期：
    3-5 working days

产品评价

靶点详情

  • 功能：
    Formation of iron-sulfur complexes, cyanide detoxification or modification of sulfur-containing enzymes. Other thiol compounds, besides cyanide, can act as sulfur ion acceptors. Also has weak mercaptopyruvate sulfurtransferase (MST) activity. Together with MRPL18, acts as a mitochondrial import factor for the cytosolic 5S rRNA. Only the nascent unfolded cytoplasmic form is able to bind to the 5S rRNA.
  • 基因功能参考文献：
    1. TST mRNA expression in adipose tissue correlated positively with insulin sensitivity in adipose tissue and negatively with fat mass. PMID: 27270587
    2. show that polymorphic variations that are distant from the active site differentially modulate the sulfurtransferase activity of human rhodanese to cyanide versus sulfite PMID: 26269602
    3. Loss of Thiosulfate Sulfurtransferase is associated with decreased mucosal sulfide detoxification capacity in patients with Crohn's disease PMID: 23399736
    4. silencing of the rhodanese gene caused not only a proportional decrease of 5 S rRNA import but also a general inhibition of mitochondrial translation, indicating the functional importance of the imported 5 S rRNA inside the organelle. PMID: 20663881
    5. Proteomics was used to study colonic epithelial aging, for differential proteins in the human normal colonic epithelial tissues from young and old people. Rack1, EF-Tu and Rhodanese, three validated differential proteins, were further investigated. PMID: 20099848
    6. A partially gluconoylated N-terminus of the His6-tagged molybdenum cofactor synthesis 3 (MOCS3)-rhodanese-like domain is identified which results in a heterogeneity of the protein but does not influence sulfurtransferase activity. PMID: 15910006
    7. The USP8 recognition domain of NRDP1 has a novel protein fold that interacts with a conserved peptide loop of the rhodanese domain. PMID: 17035239

    显示更多

    收起更多

  • 亚细胞定位：
    Mitochondrion matrix.
  • 数据库链接：

    HGNC: 12388

    OMIM: 180370

    KEGG: hsa:7263

    STRING: 9606.ENSP00000249042

    UniGene: Hs.474783