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  4. Human Transforming Growth factor β3,TGF-β3 ELISA kit

Human Transforming Growth factor β3,TGF-β3 ELISA kit

  • 中文名称：
    人转化生长因子β3(TGF-β3)酶联免疫试剂盒
  • 货号：
    CSB-E13738h
  • 规格：
    96T/48T
  • 价格：
    ￥3200/￥2500
  • 其他：

产品详情

  • 产品描述：

    This Human TGFB3 ELISA Kit was designed for the quantitative measurement of Human TGFB3 protein in serum, plasma, cell culture supernates. It is a Sandwich ELISA kit, its detection range is 15.6 pg/mL-1000 pg/mL and the sensitivity is 3.9 pg/mL.

  • 别名：
    ARVD ELISA Kit; ARVD1 ELISA Kit; FLJ16571 ELISA Kit; LDS5 ELISA Kit; MGC105479 ELISA Kit; MGC118722 ELISA Kit; prepro-transforming growth factor beta-3 ELISA Kit; RNHF ELISA Kit; TGF beta 3 ELISA Kit; TGF beta3 ELISA Kit; TGF-beta-3 ELISA Kit; TGFB 3 ELISA Kit; Tgfb3 ELISA Kit; TGFB3_HUMAN ELISA Kit; transforming growth factor beta 3 ELISA Kit; Transforming growth factor beta-3 ELISA Kit
  • 缩写：
  • Uniprot No.：
  • 种属：
    Homo sapiens (Human)
  • 样本类型：
    serum, plasma, cell culture supernates
  • 检测范围：
    15.6 pg/mL-1000 pg/mL
  • 灵敏度：
    3.9 pg/mL
  • 反应时间：
    1-5h
  • 样本体积：
    50-100ul
  • 检测波长：
    450 nm
  • 研究领域：
    Signal Transduction
  • 测定原理：
    quantitative
  • 测定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%      
    Three samples of known concentration were tested twenty times on one plate to assess.  
    Inter-assay Precision (Precision between assays): CV%<10%      
    Three samples of known concentration were tested in twenty assays to assess.    
                 
  • 线性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of human TGF-β3 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    　 Sample Serum(n=4)  
    1:1 Average % 88  
    Range % 82-95  
    1:2 Average % 97  
    Range % 94-103  
    1:4 Average % 98  
    Range % 90-104  
    1:8 Average % 87  
    Range % 82-91  
  • 回收率：
    The recovery of human TGF-β3 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample Type Average % Recovery Range  
    Serum (n=5) 92 87-98  
    EDTA plasma (n=4) 98 96-100  
                 
                 
  • 标准曲线：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    pg/ml OD1 OD2 Average Corrected  
    1000 2.699 2.752 2.726 2.571  
    500 2.435 2.399 2.417 2.262  
    250 1.808 1.875 1.842 1.687  
    125 1.065 1.055 1.060 0.905  
    62.5 0.678 0.735 0.707 0.552  
    31.2 0.401 0.422 0.412 0.257  
    15.6 0.267 0.275 0.271 0.116  
    0 0.149 0.160 0.155 　  
  • 本试剂盒所含材料：
    • A micro ELISA plate --- The 96-well plate has been pre-coated with an anti-human TGFB3 antibody. This dismountable microplate can be divided into 12 x 8 strip plates.
    • Two vials lyophilized standard ---Dilute a bottle of the standard at dilution series, read the OD values, and then draw a standard curve.
    • One vial Biotin-labeled TGFB3 antibody (100 x concentrate) (120 μl/bottle) ---Act as the detection antibody.
    • One vial HRP-avidin (100 x concentrate) (120 μl/bottle) ---Bind to the detection antibody and react with the TMB substrate to make the solution chromogenic.
    • One vial Biotin-antibody Diluent (15 ml/bottle) ---Dilute the Biotin-antibody.
    • One vial HRP-avidin Diluent (15 ml/bottle) ---Dilute the HRP-avidin solution.
    • One vial Sample Diluent (50 ml/bottle)---Dilute the sample to an appropriate concentration.
    • One vial Wash Buffer (25 x concentrate) (20 ml/bottle) ---Wash away unbound or free substances.
    • One vial TMB Substrate (10 ml/bottle) ---Act as the chromogenic agent. TMB interacts with HRP, eliciting the solution turns blue.
    • One vial Stop Solution (10 ml/bottle) ---Stop the color reaction. The solution color immediately turns from blue to yellow.
    • 1 N HCI (1 x 10 ml)--- Used for the acid activation of TGFB3 in the samples.
    • 2 N NaOH/0.5 M HEPES 1 x 10 ml---Neutralize the acidified samples.
    • Four Adhesive Strips (For 96 wells) --- Cover the microplate when incubation.
    • An instruction manual

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  • 本试剂盒不含材料：
    • A microplate reader capable of measuring absorbance at 450 nm, with the correction wavelength set at 540 nm or 570 nm.
    • An incubator can provide stable incubation conditions up to 37°C±5°C.
    • Centrifuge
    • Vortex
    • Squirt bottle, manifold dispenser, or automated microplate washer
    • Absorbent paper for blotting the microtiter plate
    • 50-300ul multi-channel micropipette
    • Pipette tips
    • Single-channel micropipette with different ranges
    • 100ml and 500ml graduated cylinders
    • Deionized or distilled water
    • Timer
    • Test tubes for dilution

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  • 数据处理：
  • 货期：
    3-5 working days

产品评价

平均分:
5.0分 - 1 个评价

样品类型：血浆（抗凝剂） EDTA

样品信息：人

稀释比：没有稀释

产品评价： ELISA结果正常组和患病组有明显差异

By 刘老师

靶点详情

  • 功能：
    Transforming growth factor beta-3 proprotein: Precursor of the Latency-associated peptide (LAP) and Transforming growth factor beta-3 (TGF-beta-3) chains, which constitute the regulatory and active subunit of TGF-beta-3, respectively.; Required to maintain the Transforming growth factor beta-3 (TGF-beta-3) chain in a latent state during storage in extracellular matrix. Associates non-covalently with TGF-beta-3 and regulates its activation via interaction with 'milieu molecules', such as LTBP1 and LRRC32/GARP, that control activation of TGF-beta-3. Interaction with integrins results in distortion of the Latency-associated peptide chain and subsequent release of the active TGF-beta-3.; Transforming growth factor beta-3: Multifunctional protein that regulates embryogenesis and cell differentiation and is required in various processes such as secondary palate development. Activation into mature form follows different steps: following cleavage of the proprotein in the Golgi apparatus, Latency-associated peptide (LAP) and Transforming growth factor beta-3 (TGF-beta-3) chains remain non-covalently linked rendering TGF-beta-3 inactive during storage in extracellular matrix. At the same time, LAP chain interacts with 'milieu molecules', such as LTBP1 and LRRC32/GARP that control activation of TGF-beta-3 and maintain it in a latent state during storage in extracellular milieus. TGF-beta-3 is released from LAP by integrins: integrin-binding results in distortion of the LAP chain and subsequent release of the active TGF-beta-3. Once activated following release of LAP, TGF-beta-3 acts by binding to TGF-beta receptors (TGFBR1 and TGFBR2), which transduce signal.
  • 基因功能参考文献：
    1. Elevations of TGF-beta3, SMAD2 and SMAD4 in hypertrophic scars and increase of IGF-1R in immature stages may give some clues for acne hypertrophic scar formation. PMID: 30167815
    2. This review highlights advances in the understanding of the cellular sources, activation processes, contextual determinants, and immunological roles of TGF-beta3 with comparisons to other TGF-beta isoforms. PMID: 30071700
    3. This study showed that YOD1 overexpression enhances cell migration by promoting TGF-beta3 signaling which may play an important role in lip and palate formation. PMID: 30145984
    4. We suggest that increased TGFbeta3 levels are responsible for development of aggressive prostate cancer in African American patients as a consequence of development of resistance to inhibitory effects of TGFbeta on cell proliferation and induction of invasive metastatic behavior PMID: 29474521
    5. data implies that miR-140 is a potent chondrogenic differentiation inducer for iPSCs and also, we have showed increasing chondrogenic differentiation by using overexpression of miR-140 and TGFbeta3. PMID: 29456027
    6. The increase in TGF-beta3 found in inflammatory wound healing (WF) highlights its negative effect on wound healing, while the increased levels of sEng in granulating WF affects the leukocyte adhesion/transmigration through the endothelium, reducing the inflammatory response and favoring the wound healing. PMID: 29065449
    7. In nonsyndromic CL+/-P Malay patients,the prevalence of the mutations in the TGFbeta3 gene was 17.7%. For the TGFbeta3 gene, there was no mutation in the coding region in either of the groups. PMID: 26151095
    8. Human dental apical papilla-derived Mesenchymal stem cells (hSCAPs) can produce and secrete TGFbeta3 in response to micro-environmental cues. PMID: 25690385
    9. Higher TGF-beta3 serum concentrations are a risk factor for uterine fibroids. PMID: 27743697
    10. High expression of TGF-beta3 in preeclampsia decidua stimulates miR-494 in decidual mesenchymal stem cells (MSC) and attenuates the regulation of MSC switching the macrophage toward M2 type, contributing to an immune imbalance at maternal-fetal interface. PMID: 27149081
    11. The frequency GA genotype of transforming growth factor beta 3 (TGFbeta3) gene was associated with increased risk of non-syndromic cleft palate only (NS CPO). PMID: 28364787
    12. combining TGF-beta3 with BMP-2 was able to promote the process of bone formation more markedly in vitro, providing a promising clinical strategy in the field of skeletal regeneration and in fracture healing. PMID: 27878265
    13. No significant association was observed in MMP13, TIMP2 and TGFB3 genes with CP or PI. CP is a risk factor to develop PI, however, there is no association of both diseases with polymorphisms in the MMP13, TIMP2 and TGFB3 genes PMID: 27058373
    14. Our study showed that TGFA/TGFB3/MSX1 gene polymorphisms were associated with congenital NSHI. CCGTAC and TTACGT haplotypes might be protective factors, while TTGCGC haplotype might be a risk factor for congenital NSHI. TGFA/TGFB3/MSX1 gene rs3771494, rs1058213, rs3917201, rs2268626, rs3821949, and rs62636562 haplotype analysis showed that haplotype CCGTAC and TTACGT might be protective factors (both P<0.001) PMID: 27356075
    15. increased stromal POSTN induced by TGF-beta3 directly accelerated the growth, migration and invasion of cancer cells PMID: 26857387
    16. Taken together, these results demonstrate that insulin and TGF-beta3 present antagonistic effects during the chondrogenesis of human bone marrow-derived stem/progenitor cells. PMID: 26866713
    17. CCN4 has a positive influence on chondrogenic differentiation by modulating the effects of TGF-beta3. PMID: 26555637
    18. TGFB3 polymorphism is associated with male infertility. PMID: 26612435
    19. Results identified a novel human TGFB3 mutation, which contributes to the clinical delineation of the emerging connective tissue disorder tentatively called Rienhoff syndrome, a disorder overlapping with Marfan and Loeys-Dietz syndrome. PMID: 26184463
    20. Fluocinolone Acetonide enhances TGF-beta3-associated chondrogenesis of bone marrow derived mesenchymal stem cells PMID: 25753754
    21. TGF-beta3-expressing CD4+CD25(-)LAG3+ regulatory T cells have a role in controlling humoral immune responses PMID: 25695838
    22. TGF-beta3 mediates the attenuating effect of MSCs on both the proliferation and extracellular matrix production of human keloid fibroblasts and decreases skin fibrosis PMID: 25858630
    23. TGF-beta3 existed in synovium and LBs of SC, and was responsible for the pathogenesis of SC. PMID: 25742744
    24. TGFB3 polymorphism, significant differences were observed for allele and genotype frequencies between caries free and caries affected individuals in oral cleft group (p = 0.013 and 0.006 for allele and genotype frequencies respectively). PMID: 25307808
    25. TGF-beta3 may contribute to the persistent intestinal dysfunction seen in gastroschisis-related intestinal dysfunction. PMID: 25431043
    26. Data indicate greater staining for transforming growth factor beta 3 (TGF-beta3) in healthier tissue compared with diseased menisci. PMID: 25418724
    27. The results show an intricate interplay between p53 and TGF-beta3 whereby p53 inhibits the TGF-beta3-induced expression of genes, e.g., EPHB2, to impede tumor cell invasion and migration PMID: 25257729
    28. findings emphasize the broad clinical variability associated with TGFB3 mutations and highlight the importance of early recognition of the disease because of high cardiovascular risk PMID: 25835445
    29. It was found that the majority of TGF-beta3 that was loaded onto the scaffold was released in a controlled manner over the first 10days of culture, with comparable long-term chondrogenesis PMID: 24907658
    30. We identified genetic variants in TGFB3 and ARHGAP29 associated with suboptimal healing outcome. PMID: 24635173
    31. These results unequivocally demonstrate that human TGF-beta3 elicits bone induction by up-regulation of endogenous BMP-2 and is blocked by hNoggin. PMID: 24438909
    32. TGF-beta3 could be stably expressed in pcDNA3.1(+)-hTGF-beta3-transfected PSCs. PMID: 21823016
    33. Liarozole decreased TGF-beta3 and TGF-beta3-mediated extracellular matrix expression in a 3D uterine leiomyoma culture system. PMID: 24825427
    34. meta-analysis suggests that TGF-beta3 gene polymorphisms may contribute to NSCLP susceptibility, especially among Asian populations. PMID: 24053560
    35. Allelic and haplotypic associations implicate a possible role of TGFB3 in nonsyndromic cleft lip with or without cleft palate in the Chilean population. PMID: 20170386
    36. This study provides a comprehensive list of genes differentially expressed in the healing corneal epithelial cells of diabetic corneas and suggests the therapeutic potential of TGF-beta3 for treating corneal and skin wounds in diabetic patients. PMID: 24306208
    37. The GG genotype and G allele of TGF-beta 3 were significantly different in the patient group compared with the control. PMID: 23023602
    38. Shear stress of vascular endothelial cells induces TGF-beta3 signaling and subsequent activation of Kruppel-like factor 2 and NO, and represents a novel role for TGF-beta3 in the maintenance of homeostasis in a hemodynamic environment. PMID: 23968981
    39. Mutation in TGFB3 is associated with a syndrome of low muscle mass, growth retardation, distal arthrogryposis and clinical features overlapping with Marfan and Loeys-Dietz syndrome. PMID: 23824657
    40. These findings suggest human serum, FGF-2 and TGF-beta3 as possible candidates to support biological treatment strategies of AF defects. PMID: 23122986
    41. These results highlight TGFbeta/5-HT signaling as a potent mechanism for control of biomechanical remodeling of atrioventricular cushions during development. PMID: 22880017
    42. Hypoxia may inhibit the invasion of human extravillous trophoblast cells through inducing the integrin switch from alpha1 integrin to alpha5 integrin and promoting TGFB3 expression. PMID: 22674391
    43. IL-1B & TGFB3 synergistically activated MMP)-1, MMP-3,& MMP-10 gene expression in NSCLC cells via MAPK-dependent pathways. PMID: 22796605
    44. Significant statistical differences were found for genotype frequencies between tooth agenesis and TGFB3 control samples, as well as for allele and genotype frequencies between unilateral tooth agenesis and TGFB3 control samples. PMID: 22191848
    45. TGF-beta3 isoform is a key agent in seminal plasma that signals induction of proinflammatory cytokine synthesis in cervical cells. PMID: 22706080
    46. analysis of genetic variants hints at the contribution of TGFB3 and MN1 in the aetiology of submucous cleft palate PMID: 22409215
    47. involved in the modulation of epithelial barrier function by regulating assembly of tight junctions PMID: 22369552
    48. This study confirms the crucial role of TGF-beta3 in the fusion of palatal shelves during development and further, provides novel evidence of TGF-beta3 gene polymorphism in the etiology of nonsyndromic cleft lip and palate in Indian subpopulation PMID: 22143699
    49. We observed significant downregulation of Transforming Growth Factor beta 3 in women with recurrent miscarriage compared with controls. PMID: 22266274
    50. study suggests that both combination medium, but better with the addition of TGF-ss3 could enhance BMSCs chondrogenesis in vivo, and promotes themaintenance of the chondrocyte phenotype PMID: 21666950

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  • 相关疾病：
    Arrhythmogenic right ventricular dysplasia, familial, 1 (ARVD1); Loeys-Dietz syndrome 5 (LDS5)
  • 亚细胞定位：
    [Latency-associated peptide]: Secreted, extracellular space, extracellular matrix.; [Transforming growth factor beta-3]: Secreted.
  • 蛋白家族：
    TGF-beta family
  • 数据库链接：

    HGNC: 11769

    OMIM: 107970

    KEGG: hsa:7043

    STRING: 9606.ENSP00000238682

    UniGene: Hs.592317