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  4. Human U3 small nucleolar ribonucleoprotein protein IMP3(IMP3) ELISA kit

Human U3 small nucleolar ribonucleoprotein protein IMP3(IMP3) ELISA kit

  • 中文名称：
    人U3小核糖核蛋白IMP3(IMP3)酶联免疫试剂盒
  • 货号：
    CSB-EL011692HU
  • 规格：
    96T/48T
  • 价格：
    ￥3600/￥2500
  • 其他：

产品详情

  • 产品描述：

    This Human IMP3 ELISA Kit was designed for the quantitative measurement of Human IMP3 protein in serum, plasma, tissue homogenates, cell lysates. It is a Sandwich ELISA kit, its detection range is 39 pg/mL-2500 pg/mL and the sensitivity is 9.75 pg/mL.

  • 别名：
    BRMS2 ELISA Kit; C15orf12 ELISA Kit; Chromosome 15 open reading frame 12 ELISA Kit; DKFZp586L0118 ELISA Kit; FLJ10968 ELISA Kit; imp3 ELISA Kit; IMP3, U3 small nucleolar ribonucleoprotein, homolog (yeast) ELISA Kit; IMP3_HUMAN ELISA Kit; Mitochondrial ribosomal protein S4 ELISA Kit; MRPS4 ELISA Kit; OTTHUMP00000183524 ELISA Kit; U3 small nucleolar ribonucleoprotein protein imp3 ELISA Kit; U3 snoRNP protein 3 homolog ELISA Kit; U3 snoRNP protein imp3 ELISA Kit
  • 缩写：
    IMP3
  • Uniprot No.：
  • 种属：
    Homo sapiens (Human)
  • 样本类型：
    serum, plasma, tissue homogenates, cell lysates
  • 检测范围：
    39 pg/mL-2500 pg/mL
  • 灵敏度：
    9.75 pg/mL
  • 反应时间：
    1-5h
  • 样本体积：
    50-100ul
  • 检测波长：
    450 nm
  • 研究领域：
    Metabolism
  • 测定原理：
    quantitative
  • 测定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%
    Three samples of known concentration were tested in twenty assays to assess.
  • 线性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of human IMP3 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    　 Sample Serum(n=4)
    1:1 Average % 95
    Range % 89-99
    1:2 Average % 97
    Range % 91-103
    1:4 Average % 92
    Range % 87-98
    1:8 Average % 89
    Range % 85-93
  • 回收率：
    The recovery of human IMP3 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample Type Average % Recovery Range
    Serum (n=5) 102 98-105
    EDTA plasma (n=4) 86 80-91
  • 标准曲线：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    pg/ml OD1 OD2 Average Corrected
    2500 2.445 2.555 2.500 2.344
    1250 1.791 1.861 1.826 1.670
    625 1.232 1.287 1.260 1.104
    312 0.866 0.877 0.872 0.716
    156 0.580 0.593 0.587 0.431
    78 0.437 0.424 0.431 0.275
    39 0.283 0.289 0.286 0.130
    0 0.155 0.156 0.156 　
  • 数据处理：
  • 货期：
    3-5 working days

产品评价

靶点详情

  • 功能：
    Component of the 60-80S U3 small nucleolar ribonucleoprotein (U3 snoRNP). Required for the early cleavages during pre-18S ribosomal RNA processing.
  • 基因功能参考文献：
    1. our study found that IMP3 expression was higher in the ovarian clear cell carcinoma than the high grade serous and endometrioid subtypes PMID: 29866423
    2. IMP3 expression in uterine leiomyosarcoma could be a marker of a poor prognosis. PMID: 29077232
    3. IMP3 positive pT1 EAC revealed higher levels of lymph node metastases. PMID: 29974234
    4. IMP3 is a strong prognostic factor in non-neuroendocrine brain metastases and in particular in patients with adenocarcinoma metastases.IMP3 expression is associated with poor prognosis in patients with non-small cell lung cancer brain metastases. PMID: 29209838
    5. Study found IMP3 frequently overexpressed in many human neoplasms and show significant associations with adverse tumor features in esophageal adenocarcinomas and cancers of the urinary bladder, lung, stomach, and pancreas. PMID: 29115542
    6. In this study, IMP3 at the nodal site resulted to be associated with low progression-free survival in small-intestine neuroendocrine neoplasms, independently of the Ki-67 index. PMID: 28210937
    7. IMP3 may contribute to the aggressive behavior of metaplastic breast carcinoma , and that this expression could potentially be a prognostic marker PMID: 29146055
    8. These findings suggest that IMP3 expression has prognostic value as a biomarker for chemosensitivity and overall survival in triple negative breast cancer PMID: 28756977
    9. This study establishes p65 as a novel target of IMP3 in increasing glioma cell migration and underscores the significance of IMP3-p65 feedback loop for therapeutic targeting in GBM. PMID: 28465487
    10. IMP3 has a similar specificity, but a better sensitivity, intensity, and extent of reactivity in comparison with AMACR, and may be used as an alternative to AMACR, in support of the diagnosis of BE-dysplasia and EAC PMID: 26766126

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  • 亚细胞定位：
    Nucleus, nucleolus.
  • 蛋白家族：
    Universal ribosomal protein uS4 family
  • 数据库链接：

    HGNC: 14497

    OMIM: 612980

    KEGG: hsa:55272

    STRING: 9606.ENSP00000326981

    UniGene: Hs.513043