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  4. Human Vascular Endothelial Growth Factor 121(VEGF121) ELISA kit

Human Vascular Endothelial Growth Factor 121(VEGF121) ELISA kit

  • 中文名称：
    人血管内皮生长因子121(VEGF121)酶联免疫试剂盒
  • 货号：
    CSB-E13709h
  • 规格：
    96T/48T
  • 价格：
    ￥3600/￥2500
  • 其他：

产品详情

  • 产品描述：

    This Human VEGF121 ELISA Kit was designed for the quantitative measurement of Human VEGF121 protein in serum, plasma, cell culture supernates, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 0.156 ng/mL-10 ng/mL and the sensitivity is 0.039 ng/mL .

  • 缩写：
    VEGF121
  • Uniprot No.：
  • 种属：
    Homo sapiens (Human)
  • 样本类型：
    serum, plasma, cell culture supernates, tissue homogenates
  • 检测范围：
    0.156 ng/mL-10 ng/mL
  • 灵敏度：
    0.039 ng/mL
  • 反应时间：
    1-5h
  • 样本体积：
    50-100ul
  • 检测波长：
    450 nm
  • 研究领域：
    Cancer
  • 测定原理：
    quantitative
  • 测定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%
    Three samples of known concentration were tested in twenty assays to assess.
  • 线性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of human VEGF121 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    　SampleSerum(n=4)
    1:1Average %98
    Range %92-106
    1:2Average %94
    Range %90-98
    1:4Average %89
    Range %86-96
    1:8Average %92
    Range %86-97
  • 回收率：
    The recovery of human VEGF121 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample TypeAverage % RecoveryRange
    Serum (n=5) 9894-104
    EDTA plasma (n=4)9187-95
  • 标准曲线：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    ng/mlOD1OD2AverageCorrected
    102.415 2.314 2.365 2.222
    51.532 1.633 1.583 1.440
    2.50.972 0.951 0.962 0.819
    1.250.558 0.547 0.553 0.410
    0.6250.404 0.411 0.408 0.265
    0.3120.285 0.274 0.280 0.137
    0.1560.226 0.220 0.223 0.080
    00.143 0.142 0.143 　
  • 数据处理：
  • 货期：
    3-5 working days

产品评价

靶点详情

  • 功能：
    Growth factor active in angiogenesis, vasculogenesis and endothelial cell growth. Induces endothelial cell proliferation, promotes cell migration, inhibits apoptosis and induces permeabilization of blood vessels. Binds to the FLT1/VEGFR1 and KDR/VEGFR2 receptors, heparan sulfate and heparin. NRP1/Neuropilin-1 binds isoforms VEGF-165 and VEGF-145. Isoform VEGF165B binds to KDR but does not activate downstream signaling pathways, does not activate angiogenesis and inhibits tumor growth. Binding to NRP1 receptor initiates a signaling pathway needed for motor neuron axon guidance and cell body migration, including for the caudal migration of facial motor neurons from rhombomere 4 to rhombomere 6 during embryonic development.
  • 相关疾病：
    Microvascular complications of diabetes 1 (MVCD1)
  • 亚细胞定位：
    Secreted. Note=VEGF121 is acidic and freely secreted. VEGF165 is more basic, has heparin-binding properties and, although a significant proportion remains cell-associated, most is freely secreted. VEGF189 is very basic, it is cell-associated after secretion and is bound avidly by heparin and the extracellular matrix, although it may be released as a soluble form by heparin, heparinase or plasmin.
  • 蛋白家族：
    PDGF/VEGF growth factor family
  • 组织特异性：
    Isoform VEGF189, isoform VEGF165 and isoform VEGF121 are widely expressed. Isoform VEGF206 and isoform VEGF145 are not widely expressed. A higher level expression seen in pituitary tumors as compared to the pituitary gland.
  • 数据库链接：

    HGNC: 12680

    OMIM: 192240

    KEGG: hsa:7422

    STRING: 9606.ENSP00000361125

    UniGene: Hs.73793