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  4. Human Vitamin D Receptor,VD R ELISA Kit

Human Vitamin D Receptor,VD R ELISA Kit

  • 中文名称：
    人维生素D受体(VD R)酶联免疫试剂盒
  • 货号：
    CSB-E05136h
  • 规格：
    96T/48T
  • 价格：
    ￥3600/￥2500
  • 其他：

产品详情

  • 产品描述：

    This Human VDR ELISA Kit was designed for the quantitative measurement of Human VDR protein in serum, plasma, tissue homogenates, cell lysates. It is a Sandwich ELISA kit, its detection range is 6.25 pg/mL-400 pg/mL and the sensitivity is 1.56 pg/mL .

  • 别名：
    1 25 dihydroxyvitamin D3 receptor ELISA Kit; 1 ELISA Kit; 1,25 dihydroxyvitamin D3 receptor ELISA Kit; 1,25-@dihydroxyvitamin D3 receptor ELISA Kit; 25-dihydroxyvitamin D3 receptor ELISA Kit; Member 1 ELISA Kit; NR1I1 ELISA Kit; Nuclear receptor subfamily 1 group I member 1 ELISA Kit; PPP1R163 ELISA Kit; Protein phosphatase 1, regulatory subunit 163 ELISA Kit; VDR ELISA Kit; VDR_HUMAN ELISA Kit; Vitamin D (1,25- dihydroxyvitamin D3) receptor ELISA Kit; Vitamin D hormone receptor ELISA Kit; Vitamin D nuclear receptor variant 1 ELISA Kit; Vitamin D receptor ELISA Kit; Vitamin D3 receptor ELISA Kit
  • 缩写：
    VDR
  • Uniprot No.：
  • 种属：
    Homo sapiens (Human)
  • 样本类型：
    serum, plasma, tissue homogenates, cell lysates
  • 检测范围：
    6.25 pg/mL-400 pg/mL
  • 灵敏度：
    1.56 pg/mL
  • 反应时间：
    1-5h
  • 样本体积：
    50-100ul
  • 检测波长：
    450 nm
  • 研究领域：
    Cancer
  • 测定原理：
    quantitative
  • 测定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%
    Three samples of known concentration were tested in twenty assays to assess.
  • 线性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of human VD R in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    　SampleSerum(n=4)
    1:1Average %90
    Range %87-96
    1:2Average %103
    Range %97-105
    1:4Average %95
    Range %90-100
    1:8Average %94
    Range %88-98
  • 回收率：
    The recovery of human VD R spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample TypeAverage % RecoveryRange
    Serum (n=5) 8982-94
    EDTA plasma (n=4)9990-105
  • 标准曲线：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    pg/mlOD1OD2AverageCorrected
    4002.178 2.098 2.138 1.977
    2001.562 1.644 1.603 1.442
    1001.138 1.118 1.128 0.967
    500.811 0.803 0.807 0.646
    250.452 0.460 0.456 0.295
    12.50.325 0.315 0.320 0.159
    6.250.227 0.231 0.229 0.068
    00.162 0.160 0.161 　
  • 本试剂盒所含材料：
    • A micro ELISA plate --The 96-well plate has been pre-coated with an anti-human VDR antibody. This dismountable microplate can be divided into 12 x 8 strip plates.
    • Two vials lyophilized standard --Dilute a bottle of the standard at dilution series, read the OD values, and then draw a standard curve.
    • One vial Biotin-labled VDR antibody (100 x concentrate) (120 μl/bottle) --Act as the detection antibody.
    • One vial HRP-avidin (100 x concentrate) (120 μl/bottle) --Bind to the detection antibody and react with the TMB substrate to make the solution chromogenic.
    • One vial Biotin-antibody Diluent (15 ml/bottle) --Dilute the high concentration Biotin-antibody to an appropriate working solution.
    • One vial HRP-avidin Diluent (15 ml/bottle) --Dilute the high concentration HRP-avidin solution to an appropriate solution.
    • One vial Sample Diluent (50 ml/bottle)--Dilute the sample to an appropriate concentration.
    • One vial Wash Buffer (25 x concentrate) (20 ml/bottle) --- Wash away unbound or free substances.
    • One vial TMB Substrate (10 ml/bottle) --Act as the chromogenic agent. TMB interacts with HRP, eliciting the solution turns blue.
    • One vial Stop Solution (10 ml/bottle) --Stop the color reaction. The solution color immediately turns from blue to yellow.
    • Four Adhesive Strips (For 96 wells) --Cover the microplate when incubation.
    • An instruction manual

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  • 本试剂盒不含材料：
    • A microplate reader capable of measuring absorbance at 450 nm, with the correction wavelength set at 540 nm or 570 nm.
    • An incubator can provide stable incubation conditions up to 37°C±5°C.
    • Centrifuge
    • Vortex
    • Squirt bottle, manifold dispenser, or automated microplate washer
    • Absorbent paper for blotting the microtiter plate
    • 50-300ul multi-channel micropipette
    • Pipette tips
    • Single-channel micropipette with different ranges
    • 100ml and 500ml graduated cylinders
    • Deionized or distilled water
    • Timer
    • Test tubes for dilution

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  • 数据处理：
  • 货期：
    3-5 working days

产品评价

问答及客户评论

■ 客户评论
  • Tested application: ELISA
    Test sample: Plasma (anticoagulant): Heparin, Tissue homogenate
    Species: Human
    Review: one-time test, for clarification.

靶点详情

  • 功能：
    Nuclear receptor for calcitriol, the active form of vitamin D3 which mediates the action of this vitamin on cells. Enters the nucleus upon vitamin D3 binding where it forms heterodimers with the retinoid X receptor/RXR. The VDR-RXR heterodimers bind to specific response elements on DNA and activate the transcription of vitamin D3-responsive target genes. Plays a central role in calcium homeostasis.
  • 基因功能参考文献：
    1. Vitamin D Receptor Gene SNPs and the environment interact to Influence survival in hemodialysis patients. PMID: 30087217
    2. Results suggest the association between some maternal VDR polymorphisms with neonatal anthropometric measures and the risk of premature birth. PMID: 30150529
    3. SNPs of the VDR and GC genes are associated with vitamin D deficiency in postmenopausal Mexican women. PMID: 30150596
    4. VDR gene FokI polymorphism is associated with papillary thyroid cancer. PMID: 30486759
    5. No significant associations were found between the VDR polymorphisms analysed and Developmental dysplasia of the hip . Further work need to be performed using genomewide analysis to elucidate the genetic basis of Developmental dysplasia of the hip . PMID: 30262704
    6. There was no significant association detected between BMI and rs1544410 of VDR in the Emirati population PMID: 29343214
    7. Apparently, VDR-mediated signaling pathways seem to be dysregulated in those pathological conditions PMID: 30096760
    8. Vitamin D Receptorgene TaqI and BsmI polymorphisms might contribute to the increased risk of hallux valgus in Chinese population. Apal or Fokl polymorphisms showed no increased susceptibility. PMID: 29705233
    9. PTPN2, an anti-inflammatory factor regulated by VDR, was reduced in type 2 diabetics with chronic kidney disease stages 1-2. PMID: 30246029
    10. ApaI gene polymorphism and Fok1 FF genotype were associated with renal cell carcinoma susceptibility in Asians PMID: 29970659
    11. findings show polymorphism Taq-1 occurring in the vitamin D receptor may have an impact on the development of acute pancreatitis due to the lack of the protective role of vitamin D. PMID: 29966312
    12. only VDR FokI polymorphism is associated with Hashimoto's thyroiditis risk in Asian population, but not in Caucasians; and the TaqI, ApaI and BsmI polymorphisms have not positive association neither in the overall population (Meta-Analysis) PMID: 28134349
    13. Loss of function VDR mutation is associated with Hereditary 1,25-dihydroxyvitamin D-resistant rickets. PMID: 29949513
    14. JNK1 and VDR act as tumor suppressors, and their stromal expression levels are associated with prognosis in esophageal squamous cell carcinoma. PMID: 29423673
    15. Associations between VDR gene polymorphisms and osteoporosis risk and bone mineral density in postmenopausal women have been documented. (Meta-analysis) PMID: 29343720
    16. Vitamin D deficiency and vitamin D receptor variants in mothers and their neonates are risk factors for neonatal sepsis PMID: 29530503
    17. Study identified that CCC and TCC VDR haplotypes are risk factors for diabetic nephropathy in patients with diabetes type 2. PMID: 30315926
    18. The VDR rs2228570 variant may increase susceptibility to dyslipidemia in the Chinese Han population. PMID: 30119682
    19. NB-UVB phototherapy is associated with improved cutaneous VDR expression and vitamin D synthesis. Better repigmentation response to NB-UVB may be related to higher baseline VDR expression and its upregulation after phototherapy PMID: 29080365
    20. Vitamin D Receptor Gene Polymorphism is associated with Breast Cancer. PMID: 28780723
    21. Studied association of vitamin D receptor (VDR) single nucleotide polymorphisms (SNPs) and promoter region deletions of toll like receptor 2 (TLR2) with genetic predisposition for pulmonary tuberculosis (PTB) in India communities. Results show that the BsmI and FokI polymorphisms of the VDR gene are significantly associated with an increased risk of PTB. PMID: 29727015
    22. Results disclose FokI polymorphism as a relevant variant capturing the association of VDR polymorphisms with viral infection. PMID: 30092343
    23. VDR (rs1544410) SNP was found to be associated with decreased serum (25[OH]D) levels. PMID: 29738868
    24. CA genotype of ApaI VDR gene polymorphism was associated with family history and C allele of ApaI was related with family history and hypercalciuria in under one-year-old infants from Turkey. PMID: 29085969
    25. A total of six Bcell epitopes and three Tcell epitopes for VDR were predicted by bioinformatics, which when validated, may in the future aid in immunological diagnosis and development of a targeted drug therapy for clinical asthma. PMID: 29901144
    26. Review/Meta-analysis: VDR Tru9I polymorphism may be associated with osteoporosis risk in Chinese individuals, but BsmI, ApaI polymorphisms might not be a risk factor for osteoporosis. PMID: 29624920
    27. Our data reveal that VDR plays a central role in protecting cells from excessive respiration and production of ROS that leads to cell damage. PMID: 29874855
    28. This study emphasizes a positive association between SNPs (Fok-I and Bsm-I) and T1DM among Saudi children with increased risk with the Fok-I F and Bsm-I b alleles. PMID: 29417618
    29. The Apa-I variant in VDR gene is associated with metabolic syndrome in southern Brazilian women with polycystic ovary syndrome. PMID: 29669566
    30. Whole blood VDR gene expression was significantly higher in the autistic disorder group compared to control subjects (p < 0.0001). There were no significant differences among allele and genotype distribution of rs11568820 and rs4516035 polymorphisms between autistic disorder patients and controls. PMID: 29777458
    31. Vitamin D receptor ApaI AC genotype may be a possible cardiovascular risk factor for the development of arteriovenous fistula failure. PMID: 29544394
    32. preliminary results indicate the VDR gene ApaI, BsmI, FokI, and TaqI polymorphisms may not be associated with elevated multiple sclerosis (MS) risk among overall populations, but ApaI polymorphism may confer different susceptibility to MS among different populations - systematic review and meta-analysis PMID: 29110148
    33. Studied association between 25-hydroxy vitamin D (25[OH]D) levels and vitamin D receptor (VDR) gene polymorphism in association with diabetes type 2. PMID: 28739347
    34. The VDR Tru9I 'uu' genotype may increase the risk of premenopausal breast cancer. PMID: 29529900
    35. Low VDR expression is associated with Coronary Artery Disease. PMID: 29176261
    36. Expression analyses showed significant downregulation of VDR expression in peripheral blood of epileptic patients compared with healthy subjects. PMID: 29549592
    37. This meta-analysis demonstrated the association between FokI and ApaI polymorphisms in VDR gene with the risk of BD, providing insights into the potential role of vitamin D receptor in the pathogenesis of BD. PMID: 29388852
    38. Vitamin D receptor polymorphisms is a risk factor for multiple sclerosis susceptibility and progression in the Czech population. PMID: 29589202
    39. important role for SOST SNP rs1877632 and VDR SNPs rs10735810 and rs731236 in the pathophysiology of stress fracture PMID: 29129460
    40. CT genotype and the C allele of VDR were significantly associated with increased risks of childhood autism spectrum disorder. PMID: 29581796
    41. Study found a significant association between multiple sclerosis and the VDR FokI polymorphism in our region of Turkey. PMID: 29331875
    42. VDR's Fok-I and Taq-I show significant association with risk of RRMS, while Apa-I and Bsm-I are not related to the risk of the disease in Iranian Kurds. PMID: 29072967
    43. The VDR rs2228570 polymorphism increases the risk of ovarian cancer in Caucasian populations in a dominant genetic model. PMID: 29239065
    44. The present study indicates an association between VDR and vitamin D binding protein Single Nucleotide Polymorphisms and Type 1 Diabetes Mellitus among Turkish subjects. PMID: 29506625
    45. Review/Meta-analysis: VDR B allele, and BB + Bb genotypes of Bsm I variant, Tt genotype of Taq I variant might be risk factors for diabetic nephropathy. PMID: 28703918
    46. The VDR Bb genotype is an independent predictor of developing secondary hyperparathyroidism in patients with end stage kidney disease. PMID: 29415666
    47. FokI and TaqI VDR variants are significantly associated with systemic lupus erythematosus in an eastern Indian cohort. PMID: 29230954
    48. examination of the evidence for the role of Vitamin D Receptor (VDR) Polymorphisms in autoimmune diseases (review). PMID: 28786260
    49. results suggests that there may be a relationship between certain VDR genotype combinations and the risk of preterm birth. PMID: 27958635
    50. VDR BsmI polymorphism was associated with decreased risk of periodontitis in Chinese individuals from South China (meta-analysis). PMID: 29208185

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  • 相关疾病：
    Rickets vitamin D-dependent 2A (VDDR2A)
  • 亚细胞定位：
    Nucleus. Cytoplasm.
  • 蛋白家族：
    Nuclear hormone receptor family, NR1 subfamily
  • 数据库链接：

    HGNC: 12679

    OMIM: 277440

    KEGG: hsa:7421

    STRING: 9606.ENSP00000447173

    UniGene: Hs.524368