Your Good Partner in Biology Research

  1. 首页
  2. 试剂盒
  3. 酶联免疫试剂盒
  4. Human Xin actin-binding repeat-containing protein 1(XIRP1) ELISA kit

Human Xin actin-binding repeat-containing protein 1(XIRP1) ELISA kit

  • 中文名称：
    人Xin肌动蛋白结合含重复序列蛋白(XIRP1)酶联免疫试剂盒
  • 货号：
    CSB-EL026195HU
  • 规格：
    96T/48T
  • 价格：
    ￥3600/￥2500
  • 其他：

产品详情

  • 产品描述：

    This Human XIRP1 ELISA Kit was designed for the quantitative measurement of Human XIRP1 protein in serum, plasma, tissue homogenates, cell lysates. It is a Sandwich ELISA kit, its detection range is 31.25 pg/mL-2000 pg/mL and the sensitivity is 7.8 pg/mL .

  • 别名：
    AI415219 ELISA Kit; Cardiomyopathy-associated protein 1 ELISA Kit; CMYA1 ELISA Kit; DKFZp451D042 ELISA Kit; DKFZp779C1255 ELISA Kit; DKFZp779C1947 ELISA Kit; MGC144145 ELISA Kit; MGC144146 ELISA Kit; Xin actin-binding repeat-containing protein 1 ELISA Kit; XIN ELISA Kit; Xirp1 ELISA Kit; XIRP1_HUMAN ELISA Kit
  • 缩写：
    XIRP1
  • Uniprot No.：
  • 种属：
    Homo sapiens (Human)
  • 样本类型：
    serum, plasma, tissue homogenates, cell lysates
  • 检测范围：
    31.25 pg/mL-2000 pg/mL
  • 灵敏度：
    7.8 pg/mL
  • 反应时间：
    1-5h
  • 样本体积：
    50-100ul
  • 检测波长：
    450 nm
  • 研究领域：
    Signal Transduction
  • 测定原理：
    quantitative
  • 测定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%
    Three samples of known concentration were tested in twenty assays to assess.
  • 线性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of human XIRP1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    　SampleSerum(n=4)
    1:1Average %96
    Range %90-102
    1:2Average %90
    Range %84-95
    1:4Average %99
    Range %95-106
    1:8Average %97
    Range %90-104
  • 回收率：
    The recovery of human XIRP1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample TypeAverage % RecoveryRange
    Serum (n=5) 8893-96
    EDTA plasma (n=4)10198-106
  • 标准曲线：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    pg/mlOD1OD2AverageCorrected
    20001.917 1.947 1.932 1.750
    10001.286 1.299 1.293 1.111
    5000.804 0.815 0.810 0.628
    2500.536 0.505 0.521 0.339
    1250.401 0.435 0.418 0.236
    62.50.312 0.323 0.318 0.136
    31.250.247 0.261 0.254 0.072
    00.181 0.183 0.182 　
  • 数据处理：
  • 货期：
    3-5 working days

产品评价

靶点详情

  • 功能：
    Protects actin filaments from depolymerization.
  • 基因功能参考文献：
    1. Abnormal CMYA1 expression affects the phosphorylation of Cx43 through the protein kinase c signaling pathway, which is involved in the regulation of gap junction intercellular communication. PMID: 29176328
    2. Aciculin interacts with filamin C and Xin and is essential for myofibril assembly. PMID: 24963132
    3. The strong correlation between the degree of muscle damage and Xin immunoreactivity suggests that Xin may be a suitable outcome measure to evaluate disease progression and treatment effects in clinical trials. PMID: 24225086
    4. We identify the SH3 domains of nebulin and nebulette as novel ligands of proline-rich regions of Xin and XIRP2. PMID: 23985323
    5. Data provide further support that ALS2CL, EPHA3, and CMYA1 are bona-fide tumor-suppressor genes and contribute to the tumorigenesis of HNSCC. PMID: 20657180
    6. Here, we identify Xin, the protein encoded by the human gene 'cardiomyopathy associated 1'(CMYA1) as filamin c binding partner at these specialized structures where the ends of myofibrils are attached to the sarcolemma PMID: 16631741
    7. Data show that in myofibrillar myopathies Xin protein exhibites significant alterations in their localization. PMID: 19151983

    显示更多

    收起更多

  • 亚细胞定位：
    Cell junction. Note=Colocalizes with actin stress fibers.
  • 蛋白家族：
    Xin family
  • 组织特异性：
    Isoform A, isoform B and isoform C are expressed in heart.
  • 数据库链接：

    HGNC: 14301

    OMIM: 609777

    KEGG: hsa:165904

    STRING: 9606.ENSP00000343140

    UniGene: Hs.447868