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  4. Human alcohol dehydrogenase,ADH ELISA Kit

Human alcohol dehydrogenase,ADH ELISA Kit

  • 中文名称：
    人乙醇脱氢酶(ADH)酶联免疫试剂盒
  • 货号：
    CSB-E11855h
  • 规格：
    96T/48T
  • 价格：
    ￥3600/￥2500
  • 其他：

产品详情

  • 产品描述：

    This Human ADH1A ELISA Kit was designed for the quantitative measurement of Human ADH1A protein in serum, plasma, cell culture supernates, tissue homogenates, cell lysates. It is a Sandwich ELISA kit, its detection range is 0.312 mU/mL-20 mU/mL and the sensitivity is 0.078 mU/mL.

  • 别名：
    ADH alpha subunit ELISA Kit; ADH ELISA Kit; ADH1 ELISA Kit; ADH1A ELISA Kit; ADH1A_HUMAN ELISA Kit; Alcohol dehydrogenase 1 (class I), alpha polypeptide ELISA Kit; Alcohol dehydrogenase 1 ELISA Kit; Alcohol dehydrogenase 1A (class I), alpha polypeptide ELISA Kit; Alcohol dehydrogenase 1A ELISA Kit; Alcohol dehydrogenase subunit alpha ELISA Kit; Aldehyde reductase ELISA Kit
  • 缩写：
    ADH1A
  • Uniprot No.：
  • 种属：
    Homo sapiens (Human)
  • 样本类型：
    serum, plasma, cell culture supernates, tissue homogenates, cell lysates
  • 检测范围：
    0.312 mU/mL-20 mU/mL
  • 灵敏度：
    0.078 mU/mL
  • 反应时间：
    1-5h
  • 样本体积：
    50-100ul
  • 检测波长：
    450 nm
  • 研究领域：
    Metabolism
  • 测定原理：
    quantitative
  • 测定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%
    Three samples of known concentration were tested in twenty assays to assess.
  • 线性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of human ADH in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    SampleSerum(n=4)
    1:1Average %83
    Range %80-87
    1:2Average %102
    Range %97-107
    1:4Average %94
    Range %90-99
    1:8Average %86
    Range %83-89
  • 回收率：
    The recovery of human ADH spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample TypeAverage % RecoveryRange
    Serum (n=5) 10299-105
    EDTA plasma (n=4)9790-100
  • 标准曲线：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    mU/mlOD1OD2AverageCorrected
    202.294 2.156 2.225 2.107
    101.778 1.698 1.738 1.620
    51.253 1.240 1.247 1.129
    2.50.797 0.775 0.786 0.668
    1.250.485 0.468 0.477 0.359
    0.6250.362 0.375 0.369 0.251
    0.3120.256 0.262 0.259 0.141
    00.119 0.117 0.118
  • 数据处理：
  • 货期：
    3-5 working days

产品评价

靶点详情

  • 基因功能参考文献：
    1. High ADH expression is associated with Prostate Cancer. PMID: 28870918
    2. Results suggest a potential role of alcohol dehydrogenase (ADH) class I as a marker for renal cell carcinoma (RCC). PMID: 27086037
    3. Data indicate that the up-regulation of aldehyde dehydrogenase-1 (ALDH1) after neoadjuvant chemotherapy (NAC) predicts poor survival in locally advanced breast cancer. PMID: 24762066
    4. Unde-rexpression of the ADH1 gene, which influences the transformation of the extracellular matrix, plays a probable role in the etiology of uterine fibroid. PMID: 23891545
    5. high levels of ADH1 transcription are implicated in fluconazole resistance in C. albicans and that the mRNA expression levels of ADH1 are positively correlated with those of CDR1, CDR2 and FLU1. PMID: 23769565
    6. common ADH variants conferred risk for both schizophrenia in African-Americans and autism in European-Americans. PMID: 23468174
    7. High ADH1 is associated with endometrial cancer. PMID: 20872569
    8. ADH1A variation predisposes to personality traits and substance dependence PMID: 19526455
    9. Structural aspects that influence dimer-tetramer formation. PMID: 12081471
    10. GATA-2 and HNF-3beta regulate the human alcohol dehydrogenase 1A (ADH1A) gene. PMID: 16153155
    11. The oxidative pathway of ethanol metabolism via ADH and ALDH does not play a role in pancreatic carcinogenesis. PMID: 17632320
    12. Alleles of ADH7 SNPs were associated with the early stages of alcohol metabolism, with additional effects in the ADH1A, ADH1B and ADH4 regions. PMID: 19193628
    13. Observational study of healthcare-related. (HuGE Navigator) PMID: 15279067

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  • 亚细胞定位：
    Cytoplasm.
  • 蛋白家族：
    Zinc-containing alcohol dehydrogenase family
  • 数据库链接：

    HGNC: 249

    OMIM: 103700

    KEGG: hsa:124

    STRING: 9606.ENSP00000209668

    UniGene: Hs.654433