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  4. Human alpha-fetoprotein Lens culinaris agglutiin 3,AFP-L3 ELISA Kit

Human alpha-fetoprotein Lens culinaris agglutiin 3,AFP-L3 ELISA Kit

  • 中文名称：
    人小扁豆素结合型甲胎蛋白/甲胎蛋白异质体3(AFP-L3)酶联免疫试剂盒
  • 货号：
    CSB-E09058h
  • 规格：
    96T/48T
  • 价格：
    ￥3600/￥2500
  • 其他：

产品详情

  • 产品描述：

    This Human AFP-L3 ELISA Kit was designed for the quantitative measurement of Human AFP-L3 protein in serum, plasma, tissue homogenates, cell lysates. It is a Sandwich ELISA kit, its detection range is 0.312 ng/mL-20 ng/mL and the sensitivity is 0.3 ng/mL.

  • 缩写：
    AFP-L3
  • 种属：
    Homo sapiens (Human)
  • 样本类型：
    serum, plasma, tissue homogenates, cell lysates
  • 检测范围：
    0.312 ng/mL-20 ng/mL
  • 灵敏度：
    0.3 ng/mL
  • 反应时间：
    1-5h
  • 样本体积：
    50-100ul
  • 检测波长：
    450 nm
  • 研究领域：
    Cardiovascular
  • 测定原理：
    quantitative
  • 测定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<10%        
    Three samples of known concentration were tested twenty times on one plate to assess.    
    Inter-assay Precision (Precision between assays): CV%<15%        
    Three samples of known concentration were tested in twenty assays to assess.      
                   
  • 线性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of human AFP-L3 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.  
    　 Sample Serum(n=4)    
    1:1 Average % 84    
    Range % 80-92    
    1:2 Average % 97    
    Range % 91-105    
    1:4 Average % 97    
    Range % 92-110    
    1:8 Average % 93    
    Range % 86-98    
  • 回收率：
    The recovery of human AFP-L3 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.  
     
    Sample Type Average % Recovery Range    
    Serum (n=5) 95 89-98    
    EDTA plasma (n=4) 97 90-104    
                   
                   
  • 标准曲线：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.  
     
    ng/ml OD1 OD2 Average      
    80 2.207 2.356 2.282      
    40 1.205 1.213 1.209      
    20 0.648 0.639 0.644      
    10 0.318 0.324 0.321      
    4 0.160 0.159 0.160      
    2 0.098 0.095 0.097      
  • 数据处理：
  • 货期：
    3-5 working days

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