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Human amyloid beta peptide 1-42,Aβ1-42 ELISA Kit

  • 中文名称：
    人β淀粉样蛋白1-42(Aβ1-42)酶联免疫试剂盒
  • 货号：
    CSB-E10684h
  • 规格：
    96T/48T
  • 价格：
    ￥3600/￥2500
  • 其他：

产品详情

  • 产品描述：
        Aβ1-42是beta淀粉样蛋白（Aβ）的一种形式，它是一种由神经元合成和分泌的蛋白质。Aβ1-42是导致阿尔茨海默病（AD）的重要成分之一。它在AD病变过程中聚集成胶质斑和神经纤维缠结，损害神经元的正常功能。此外，Aβ1-42还可能在其他神经系统疾病的发病机制中发挥作用，包括创伤性脑损伤、脊髓灰质炎和肌萎缩侧索硬化症等。
        j9九游会登录入口首页生物所提供的Human amyloid beta peptide 1-42,Aβ1-42 ELISA Kit属于ELISA检测试剂盒，采用双抗夹心法定量检测人血清、血浆、组织匀浆、脑脊液(CSF)样本中的Aβ1-42，其灵敏度为0.078 ng/mL，检测范围为0.312 ng/mL-20 ng/mL。
     
  • 别名：
    Amyloid beta 42(ABeta 42),amyloid beta peptide 1-42,Aβ1-42,amyloid βpeptide 1-42
  • 缩写：
    Aβ1-42
  • 种属：
    Homo sapiens (Human)
  • 样本类型：
    serum, plasma, tissue homogenates, cerebrospinal fluid (CSF)
  • 检测范围：
    0.312 ng/mL-20 ng/mL
  • 灵敏度：
    0.078 ng/mL
  • 反应时间：
    1-5h
  • 样本体积：
    50-100ul
  • 检测波长：
    450 nm
  • 研究领域：
    Neuroscience
  • 测定原理：
    quantitative
  • 测定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%      
    Three samples of known concentration were tested twenty times on one plate to assess.  
    Inter-assay Precision (Precision between assays): CV%<10%      
    Three samples of known concentration were tested in twenty assays to assess.    
                 
  • 线性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of human Aβ1-42 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    　 Sample Serum(n=4)  
    1:1 Average % 84  
    Range % 80-92  
    1:2 Average % 97  
    Range % 91-105  
    1:4 Average % 97  
    Range % 92-110  
    1:8 Average % 93  
    Range % 86-98  
  • 回收率：
    The recovery of human Aβ1-42 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample Type Average % Recovery Range  
    Serum (n=5) 95 89-98  
    EDTA plasma (n=4) 97 90-100  
                 
                 
  • 标准曲线：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    ng/ml OD1 OD2 Average Corrected  
    20 2.302 2.379 2.341 2.244  
    10 1.762 1.781 1.772 1.675  
    5 1.124 1.189 1.157 1.060  
    2.5 0.653 0.679 0.666 0.569  
    1.25 0.375 0.398 0.387 0.290  
    0.625 0.251 0.259 0.255 0.158  
    0.312 0.151 0.153 0.152 0.055  
    0 0.095 0.098 0.097 　  
  • 本试剂盒所含材料：
      • A micro ELISA plate --- The 96-well plate has been pre-coated with anti-human Aβ1-42 antibody. This dismountable microplate can be divided into 12 x 8 strip plates.
      • Two vials lyophilized standard --- Dilute a bottle of standard at dilution series, read the OD values, and then draw a standard curve.
      • One vial Biotin-labeled Aβ1-42 antibody (100 x concentrate) (120 μl/bottle) ---Act as the detection antibody.
      • One vial HRP-avidin (100 x concentrate) (120 μl/bottle) --- Bind to the detection antibody and react with the TMB substrate to make the solution chromogenic.
      • One vial Biotin-antibody Diluent (15 ml/bottle) ---Dilute the high concentration Biotin-antibody to an appropriate working solution.
      • One vial HRP-avidin Diluent (15 ml/bottle) ---Dilute the high concentration HRP-avidin solution to an appropriate solution.
      • One vial Sample Diluent (50 ml/bottle)---Dilute the sample to an appropriate concentration.
      • One vial Wash Buffer (25 x concentrate) (20 ml/bottle) --- Wash away unbound or free substances.
      • One vial TMB Substrate (10 ml/bottle) --- Act as the chromogenic agent. TMB interacts with HRP, eliciting the solution turns blue.
      • One vial Stop Solution (10 ml/bottle) --- Stop the color reaction. The solution color immediately turns from blue to yellow.
      • Four Adhesive Strips (For 96 wells) --- Cover the microplate when incubation.
      • An instruction manual

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  • 本试剂盒不含材料：
      • A microplate reader capable of measuring absorbance at 450 nm, with the correction wavelength set at 540 nm or 570 nm.
      • An incubator can provide stable incubation conditions up to 37°C±5°C.
      • Centrifuge
      • Vortex
      • Squirt bottle, manifold dispenser, or automated microplate washer
      • Absorbent paper for blotting the microtiter plate
      • 50-300ul multi-channel micropipette
      • Pipette tips
      • Single-channel micropipette with different ranges
      • 100ml and 500ml graduated cylinders
      • Deionized or distilled water
      • Timer
      • Test tubes for dilution

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  • 数据处理：
  • 货期：
    3-5 working days

引用文献

产品评价

问答及客户评论

■ 常见问题解答
Q:

We are interested in following item.
#CSB-E10684h
Do you have the information of capture antibody coated on the plate and biotinylated detection antibody in the kit?

A:
Thanks for your inquiry.Regarding the mentioned kit,we would like to inform the following information:
(Note:Capture:capture antibody coated on the plate;Detection: biotin-conjugated antibody)
#CSB-E10684h,Human amyloid beta peptide 1-42,Aβ1-42 ELISA Kit
Capture:mouse monoclonal antibody;Detection:goat polyclonal antibody
■ 客户评论
  • Sample type: Brain tissues
    Sample species: Human
    Review: the discrimination index in the object recognition test. After the MWM test and the object recognition test, animals were sacrificed and the brain tissues were collected. Aβ42 levels in the cortex (K) and hippocampus (L) were detected by ELISA. # p < 0.05, ###p < 0.001 vs. Wt group. * p < 0.05, **p < 0.01, ***p < 0.001 vs. APP/PS1 group.
    PMID: 30128052

靶点详情

  • 最新研究进展：
    Aβ1-42是β淀粉样蛋白（amyloid beta protein，Aβ）的一种亚型，它是导致阿尔茨海默病（Alzheimer's disease，AD）的主要病理因素之一。最新的研究表明，Aβ1-42的聚集和沉积不仅在AD中发挥作用，而且在其他神经退行性疾病中也可能具有重要作用，如前额颞叶痴呆症、帕金森氏病等。