Human angiotensin Ⅰ,Ang-Ⅰ ELISA Kit

产品详情

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Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

产品描述：

This Human Ang-Ⅰ ELISA Kit was designed for the quantitative measurement of Human Ang-Ⅰ protein in serum, plasma, cell culture supernates. It is a Sandwich ELISA kit, its detection range is 15.6 pg/mL-1000 pg/mL and the sensitivity is 3.9 pg/mL.
别名：

angiotensin Ⅰ,Ang-Ⅰ,angiotensin 1,Ang-1
缩写：

Ang-Ⅰ
种属：

Homo sapiens (Human)
样本类型：

serum, plasma, cell culture supernates
检测范围：

15.6 pg/mL-1000 pg/mL
灵敏度：

3.9 pg/mL
反应时间：

1-5h
样本体积：

50-100ul
检测波长：

450 nm
研究领域：

Cardiovascular
测定原理：

quantitative
测定方法：

Sandwich

精密度：

线性度：

To assess the linearity of the assay, samples were spiked with high concentrations of human Ang-Ⅰ in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	100
	Range %	97-105
1:2	Average %	98
	Range %	94-103
1:4	Average %	105
	Range %	100-110
1:8	Average %	108
	Range %	102-114

回收率：

The recovery of human Ang-Ⅰ spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	92	87-97
EDTA plasma (n=4)	95	90-101

标准曲线：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.