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中文名称:人血管紧张素Ⅱ受体1抗体(ATⅡR1)酶联免疫试剂盒
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货号:CSB-E10374h
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规格:96T/48T
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价格:¥3200/¥2500
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其他:
产品详情
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产品描述:
This Human ATⅡR1 Ab ELISA Kit was designed for the quantitative measurement of Human ATⅡR1 Ab protein in serum, plasma, tissue homogenates, cell lysates. It is a Sandwich ELISA kit, its detection range is 0.312 ng/mL-20 ng/mL and the sensitivity is 0.078 ng/mL.
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别名:angiotensin 2 receptor 1 antibody, AT2R1-Ab
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缩写:ATⅡR1 Ab
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种属:Homo sapiens (Human)
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样本类型:serum, plasma, tissue homogenates, cell lysates
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检测范围:0.312 ng/mL-20 ng/mL
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灵敏度:0.078 ng/mL
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反应时间:1-5h
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样本体积:50-100ul
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检测波长:450 nm
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研究领域:Cardiovascular
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测定原理:quantitative
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测定方法:Sandwich
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精密度:
Intra-assay Precision (Precision within an assay): CV%<8% Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision (Precision between assays): CV%<10% Three samples of known concentration were tested in twenty assays to assess. -
线性度:
To assess the linearity of the assay, samples were spiked with high concentrations of human ATⅡR1 antibody in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. Sample Serum(n=4) 1:1 Average % 95 Range % 87-105 1:2 Average % 94 Range % 84-100 1:4 Average % 98 Range % 92-101 1:8 Average % 94 Range % 82-105 -
回收率:
The recovery of human ATⅡR1 antibody spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. Sample Type Average % Recovery Range Serum (n=5) 95 83-105 EDTA plasma (n=4) 95 85-103 -
标准曲线:
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. ng/ml OD1 OD2 Average Corrected 20 2.612 2.662 2.637 2.491 10 1.973 1.939 1.956 1.810 5 1.294 1.304 1.299 1.153 2.5 0.772 0.754 0.763 0.617 1.25 0.474 0.498 0.486 0.340 0.625 0.346 0.354 0.350 0.204 0.312 0.255 0.254 0.255 0.109 0 0.147 0.145 0.146 -
数据处理:
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货期:3-5 working days
引用文献
- AT1AA (Angiotensin II Type-1 Receptor Autoantibodies) Cause or Consequence of Human Primary Aldosteronism? Piazza M, et al,Hypertension,2019
- De novo development of antibodies to kidney-associated self-antigens angiotensin II receptor type I, collagen IV, and fibronectin occurs at early time points after kidney transplantation in children. Hesemann LE1, Subramanian V2, Mohanakumar T2, Dharnidharka VR1,Pediatr Transplant.,2015
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