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Human blca-4 ELISA Kit

  • 中文名称：
    人blca-4酶联免疫试剂盒
  • 货号：
    CSB-E14959h
  • 规格：
    96T/48T
  • 价格：
    ￥3600/￥2500
  • 其他：

产品详情

  • 产品描述：

    This Human BLCA-4 ELISA Kit was designed for the quantitative measurement of Human BLCA-4 protein in serum, plasma, urine, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 0.625 ng/mL-40 ng/mL and the sensitivity is 0.156 ng/mL.

  • 缩写：
    BLCA-4
  • 种属：
    Homo sapiens (Human)
  • 样本类型：
    serum, plasma, urine, tissue homogenates
  • 检测范围：
    0.625 ng/mL-40 ng/mL
  • 灵敏度：
    0.156 ng/mL
  • 反应时间：
    1-5h
  • 样本体积：
    50-100ul
  • 检测波长：
    450 nm
  • 研究领域：
    Others
  • 测定原理：
    quantitative
  • 测定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%      
    Three samples of known concentration were tested twenty times on one plate to assess.  
    Inter-assay Precision (Precision between assays): CV%<10%      
    Three samples of known concentration were tested in twenty assays to assess.    
                 
  • 线性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of human blca-4 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
      Sample Serum(n=4)  
    1:1 Average % 87  
    Range % 84-91  
    1:2 Average % 99  
    Range % 96-103  
    1:4 Average % 104  
    Range % 101-107  
    1:8 Average % 95  
    Range % 89-99  
  • 回收率：
    The recovery of human blca-4 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample Type Average % Recovery Range  
    Serum (n=5) 87 84-90  
    EDTA plasma (n=4) 92 88-96  
                 
                 
  • 标准曲线：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    ng/ml OD1 OD2 Average Corrected  
    40 2.595 2.540 2.568 2.452  
    20 2.208 2.365 2.287 2.171  
    10 1.823 1.804 1.814 1.698  
    5 1.195 1.175 1.185 1.069  
    2.5 0.698 0.678 0.688 0.572  
    1.25 0.418 0.432 0.425 0.309  
    0.625 0.271 0.276 0.274 0.158  
    0 0.115 0.117 0.116    
  • 数据处理：
  • 货期：
    3-5 working days

产品评价

问答及客户评论

■ 常见问题解答
Q:

I am interested in purchasing the kit for a study that we wanted to perform. Can you please tell me more abou the antibodies that comprise this kit?

A:
Thanks for your inquiry.
CSB-E14959h The kit uses double antibody sandwich method to quatitative detect blca-4.
And its coated antibody is goat polyclonal antibody, biotin-conjugated antibody (that is, detection antibody) is goat polyclonal antibody
As for the specific components of the kit can be seen in the manual MATERIALS PROVIDED part.I will send the manual to you by e-mail. Pls let me know if you have any further questions. Thank you.