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  4. Human cross linked N-telopeptide of type Ⅰ collagen,NTX ELISA Kit

Human cross linked N-telopeptide of type Ⅰ collagen,NTX ELISA Kit

  • 中文名称：
    人Ⅰ型胶原N末端肽(NTX)酶联免疫试剂盒
  • 货号：
    CSB-E09233h
  • 规格：
    96T/48T
  • 价格：
    ￥3200/￥2500
  • 其他：

产品详情

  • 产品描述：

    This Human NTX ELISA Kit was designed for the quantitative measurement of Human NTX protein in serum, plasma, urine. It is a Sandwich ELISA kit, its detection range is 7.8 nM BCE-500 nM BCE and the sensitivity is 1.95 nM BCE.

  • 别名：
    cross linked N-telopeptide of type 1 collagen,NTX,_x000D_ cross linked N-telopeptide of type I collagen,NTX
  • 缩写：
    NTX
  • 种属：
    Homo sapiens (Human)
  • 样本类型：
    serum, plasma, urine
  • 检测范围：
    7.8 nM BCE-500 nM BCE
  • 灵敏度：
    1.95 nM BCE
  • 反应时间：
    1-5h
  • 样本体积：
    50-100ul
  • 检测波长：
    450 nm
  • 研究领域：
    Neuroscience
  • 测定原理：
    quantitative
  • 测定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%      
    Three samples of known concentration were tested twenty times on one plate to assess.  
    Inter-assay Precision (Precision between assays): CV%<10%      
    Three samples of known concentration were tested in twenty assays to assess.    
                 
  • 线性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of human NTX in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    　 Sample Serum(n=4)  
    1:1 Average % 87  
    Range % 82-93  
    1:2 Average % 98  
    Range % 94-102  
    1:4 Average % 102  
    Range % 98-106  
    1:8 Average % 95  
    Range % 92-99  
  • 回收率：
    The recovery of human NTX spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample Type Average % Recovery Range  
    Serum (n=5) 91 85-106  
    EDTA plasma (n=4) 92 88-101  
                 
                 
  • 标准曲线：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    nM BCE OD1 OD2 Average Corrected  
    500 1.983 1.976 1.980 1.850  
    250 1.446 1.550 1.498 1.368  
    125 1.011 1.093 1.052 0.922  
    62.5 0.616 0.625 0.621 0.491  
    31.25 0.374 0.361 0.368 0.238  
    15.6 0.272 0.274 0.273 0.143  
    7.8 0.204 0.215 0.210 0.080  
    0 0.131 0.129 0.130 　  
  • 数据处理：
  • 货期：
    3-5 working days

产品评价

问答及客户评论

■ 常见问题解答
Q:

Can you run this assay on samples (whole blood or serum) that has been previously frozen or do samples have to be fresh?

A:
Thanks for your inquiry.
CSB-E09233h
This kit can test serum and plasma samples( not whole blood). It's better to test fresh samples；
If the sample has been previously frozen, pls pay attention to the storage time and avoid repeat freezing and thawing. Repeat freezing and thawing will destroy the structure of protein and affects detection. We suggest you do a pretest first if you want to test frozen sample.