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  4. Human cystathionine β-synthase(CBS) ELISA Kit

Human cystathionine β-synthase(CBS) ELISA Kit

  • 中文名称：
    人胱硫醚β-合酶(CBS)酶联免疫试剂盒
  • 货号：
    CSB-E13314h
  • 规格：
    96T/48T
  • 价格：
    ￥3600/￥2500
  • 其他：

产品详情

  • 产品描述：

    This Human CBS ELISA Kit was designed for the quantitative measurement of Human CBS protein in serum, plasma, tissue homogenates, cell lysates. It is a Sandwich ELISA kit, its detection range is 125 pg/mL-8000 pg/mL and the sensitivity is 31.25 pg/mL.

  • 别名：
    AI047524 ELISA Kit; AI303044 ELISA Kit; Beta thionase ELISA Kit; Beta-thionase ELISA Kit; Cbs ELISA Kit; Cbs cystathionine beta-synthase ELISA Kit; CBS_HUMAN ELISA Kit; Cystathionine beta synthase ELISA Kit; Cystathionine beta-synthase ELISA Kit; EC 4.2.1.22 ELISA Kit; HIP 4 ELISA Kit; HIP4 ELISA Kit; Methylcysteine synthase ELISA Kit; MGC18856 ELISA Kit; MGC18895 ELISA Kit; MGC37300 ELISA Kit; OTTHUMP00000109416 ELISA Kit; OTTHUMP00000109418 ELISA Kit; Serine sulfhydrase ELISA Kit
  • 缩写：
    CBS
  • Uniprot No.：
  • 种属：
    Homo sapiens (Human)
  • 样本类型：
    serum, plasma, tissue homogenates, cell lysates
  • 检测范围：
    125 pg/mL-8000 pg/mL
  • 灵敏度：
    31.25 pg/mL
  • 反应时间：
    1-5h
  • 样本体积：
    50-100ul
  • 检测波长：
    450 nm
  • 研究领域：
    Metabolism
  • 测定原理：
    quantitative
  • 测定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%
    Three samples of known concentration were tested in twenty assays to assess.
  • 线性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of human CBS in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    　 Sample Serum(n=4)
    1:1 Average % 94
    Range % 90-98
    1:2 Average % 106
    Range % 102-110
    1:4 Average % 96
    Range % 90-101
    1:8 Average % 88
    Range % 86-96
  • 回收率：
    The recovery of human CBS spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample Type Average % Recovery Range
    Serum (n=5) 103 98-106
    EDTA plasma (n=4) 94 91-97
  • 标准曲线：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    pg/ml OD1 OD2 Average Corrected
    8000 2.144 2.033 2.089 1.978
    4000 1.388 1.411 1.400 1.289
    2000 0.932 0.989 0.961 0.850
    1000 0.655 0.636 0.646 0.535
    500 0.445 0.424 0.435 0.324
    250 0.266 0.268 0.267 0.156
    125 0.212 0.221 0.217 0.106
    0 0.110 0.112 0.111 　
    .
  • 数据处理：
  • 货期：
    3-5 working days

产品评价

靶点详情

  • 功能：
    Hydro-lyase catalyzing the first step of the transsulfuration pathway, where the hydroxyl group of L-serine is displaced by L-homocysteine in a beta-replacement reaction to form L-cystathionine, the precursor of L-cysteine. This catabolic route allows the elimination of L-methionine and the toxic metabolite L-homocysteine. Also involved in the production of hydrogen sulfide, a gasotransmitter with signaling and cytoprotective effects on neurons.
  • 相关疾病：
    Cystathionine beta-synthase deficiency (CBSD)
  • 亚细胞定位：
    Cytoplasm. Nucleus.
  • 蛋白家族：
    Cysteine synthase/cystathionine beta-synthase family
  • 组织特异性：
    In the adult strongly expressed in liver and pancreas, some expression in heart and brain, weak expression in lung and kidney. In the fetus, expressed in brain, liver and kidney.
  • 数据库链接：

    HGNC: 1550

    OMIM: 236200

    KEGG: hsa:102724560

    UniGene: Hs.533013