Human deoxyribonuclease Ⅰ,DNase-Ⅰ ELISA Kit

Serum endocuclease secreted into body fluids by a wide variety of exocrine and endocrine organs. Expressed by non-hematopoietic tissues and preferentially cleaves protein-free DNA. Among other functions, seems to be involved in cell death by apoptosis. Binds specifically to G-actin and blocks actin polymerization. Together with DNASE1L3, plays a key role in degrading neutrophil extracellular traps (NETs). NETs are mainly composed of DNA fibers and are released by neutrophils to bind pathogens during inflammation. Degradation of intravascular NETs by DNASE1 and DNASE1L3 is required to prevent formation of clots that obstruct blood vessels and cause organ damage following inflammation.

1. Genetic variants in DNase I hypersensitive sites play an important role in carcinogenesis. two novel single nucleotide polymorphisms rs12309362 (odds ratio = 0.64, P = 5.61 x 10(-6) ) and rs9970827 (odds ratio = 0.73, P = 7.23 x 10(-6) ) significantly associated with decreased risk of HCC. PMID: 28321907
2. Serum DNase1-activity is significantly decreased in ALD patients, indicating its potential implication in their pathogenesis. Furthermore, DNase1-activity could be used as a new surrogate biomarker for predicting response to AIH treatment. PMID: 28263100
3. Although no significant association between Q222R polymorphism in DNAse I gene and the risk of systemic lupus erythematosus was found, the presence of the A allele was associated with an increased risk for the development of nephropathy PMID: 27606473
4. DNase I activity was observed to be increased in type 2 diabetes, and high glucose combined with increased DNase I is suggested to aggravate beta-cell apoptosis. PMID: 27082840
5. Single nucleotide polymorphisms producing a loss-of-function variant of the enzymes in DNASE1, DNASE1L3, and DNASE2, possibly serving as a genetic risk factor for autoimmune diseases, were confirmed. PMID: 27116004
6. in Iranians the 3/6 genotype frequency was significantly higher in systemic lupus erythematosus patients than in healthy controls which implies the possible role of this genotype in SLE susceptibility; the 3/4 and 4/6 genotype frequencies were remarkably high in the control group which indicated the protective role of these genotypes against the disease PMID: 26547219
7. TNF-alpha amplifies DNaseI expression in renal tubular cells while IL-1beta promotes nuclear DNaseI translocation in inactive form in lupus nephritis. PMID: 26065428
8. HumDN1 polymorphisms were examined in blood of 11 worldwide populations by polymerase chain reaction. 15 genotypes were found leading to the conclusion that HumDN1 variable no. tandem repeats are ethnic group specific. PMID: 25771153
9. Val66Met in the BDNF gene and two SNPs, Fokl and Apal, in the VDR gene may potentially be associated with DED. Additionally, the association between DED and Val66Met may vary by depression status. PMID: 26393465
10. In conclusion, elevated DNase I in diabetes may be related to pancreatic injury and could be one of the causes that induce diabetes. PMID: 24676545
11. Our results indicate that increased DNASE1 expression is common to both SLE and RA, while DNase1 reduction activity is specific to SLE. PMID: 24564745
12. Characterization of the nonsynonymous single-nucleotide polymorphism variants of human DNASE1. PMID: 24819173
13. DNase I activity in systemic lupus erythematosus patients was lower than in healthy controls PMID: 23183758
14. DNAse I Q222R polymorphism is a potential genetic risk factor for systemic lupus erythematosus in South Indian Tamils. In addition, the mutant allele confers a significant risk for lupus nephritis. PMID: 23963431
15. Single nucleotide polymorphisms in the DNASE1 is associated with autoimmune diseases. PMID: 24206041
16. a downregulation of the DNASE1 mRNA expression in patients with autoimmune thyroid disease. This might result in degrading less DNA from dying cells, thereby promoting the development of thyroid autoimmunity. PMID: 23225239
17. Early mesangial nephritis initiates a cascade of inflammatory signals that lead to up-regulation of Trap1 and a consequent down-regulation of renal DNaseI by transcriptional interference. PMID: 23273922
18. The novel structure of rhDNase I presented herein reveals the precise location and coordination sphere of the Mg2+ ion bound at subsite IVb along with the orientation of key side chains in the active site. PMID: 23215638
19. silencing of renal DNaseI gene expression initiates a cascade of inflammatory signals including activation of Toll like receptors and Clec4e, leading to progression of both murine and human lupus nephritis PMID: 22479529
20. POU1F1 binds to multiple sites at deoxyribonuclease I hypersensitive site II. PMID: 22094313
21. Modified DNase1 can efficiently eliminate apoptosis-resistant cancer cells through apoptosis. PMID: 21233855
22. Show no particular variant in exon2 sequence of DNASE1 gene among Tunisian patients affected with systemic lupus erythematosus, rheumatoid arthritis and Sjogren syndrome and healthy subjects. PMID: 19360410
23. The genetic aspects of DNASE1 with regard to all the SNPs in wide-ranging ethnic groups could be first demonstrated. Further, there was no correlation of all the polymorphic SNPs other than nonsynonymous ones with serum DNase I activity levels. PMID: 21235399
24. In thymocyte-selected CD4 T cells (T-CD4 T cells) DNase I hypersensitive sites at the 3'-enhancer region of interleukin-4 play an essential role during the induction of IL-4 expression. PMID: 21282512
25. Low DNase1 activity is associated to the active phase of type III or IV lupus erythromatosus nephropathy. PMID: 19844716
26. Reduction in renal Dnase1 expression and activity is limited to mice and SLE patients with signs of membranoproliferative nephritis, and may be a critical event in the development of severe forms of lupus nephritis. PMID: 20856893
27. The objectives of this study were to clarify genetic and biochemical aspects of 12 non-synonymous SNPs in the human gene (DNASE1), potentially giving rise to an alteration in the in vivo DNase I activity levels. PMID: 20417303
28. show that serum endonuclease DNase1 is essential for disassembly of neutrophil extracellular traps. PMID: 20439745
29. A significant association of HumDN1 Variable Number of Tandem Repeats polymorphism in DNASE1 gene with systemic lupus erythematosus, is reported. PMID: 19863681
30. results show that the chief cells of the stomach produce DNase I PMID: 12005024
31. Deficiencies in this gene may contribute to SLE; gene therapy might be possible (REVIEW) PMID: 12708782
32. The A/T mutation of the DNASE1 gene is absent in both Tunisian systemic lupus erythematosus and in controls. PMID: 14613299
33. The studies of immunological properties and the tissue-distribution patterns of DNase I indicated that the canine enzyme is more closely related to the human DNase I than to other mammalian DNases I PMID: 14688237
34. serum Dnase1 in cooperation with the plasminogen system guarantees a fast and effective breakdown of chromatin during necrosis by the combined cleavage of DNA as well as of DNA binding proteins PMID: 15188364
35. Results describe chromatin disposal during necrosis and the involvement of Deoxyribonuclease 1 in this process with respect to its possible role in the prevention of anti-nuclear auto-immunity. PMID: 16352456
36. human DNASE1 expression is regulated through the use of alternative promoter and alternative splicing PMID: 16771825
37. This study is the first to reveal an extremely high frequency of DNASE1*1 among African populations. Caucasians and Asians had a lower frequency of DNASE1*1 than the African groups. PMID: 17405189
38. Compared to other ethnic populations, Han Chinese had its own unique DNase I gene distribution characteristics PMID: 17588132
39. Han Chinese myocardial infarction (MI) patients, but deoxyribonuclease I gene polymorphisms are not associated with susceptibility to MI in Han Chinese PMID: 18311594
40. In patients with autoimmune thyroid disease (AITD), a novel mutation (1218G>A, exon 5) and multiple polymorphisms were identified in the DNASE1 gene. PMID: 19022625
41. DNASE1 polymorphism appears to affect the specific activity, heat sensitivity, and optimum pH of the DNase I enzyme. PMID: 19055475
42. human recombinant DNase I up-regulates cell surface Fas expression and induces increased susceptibility of T cells to Fas-mediated apoptosis PMID: 19181929
43. DNase1 (deoxyribonuclease I) activity was significantly lower in patients with Systemic lupus erythematosus than in controls. PMID: 19318394
44. deficiency is linked to systemic lupus erythematosus PMID: 19362700

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Systemic lupus erythematosus (SLE)

Secreted. Zymogen granule. Nucleus envelope. Note=Secretory protein, stored in zymogen granules and found in the nuclear envelope.

DNase I family

Principally in tissues of the digestive system. Highest levels found in urine, but also relatively abundant in semen and saliva.

HGNC: 2956

OMIM: 125505

KEGG: hsa:1773

STRING: 9606.ENSP00000246949

UniGene: Hs.629638