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  4. Human free haemoglobin,f-Hb ELISA Kit

Human free haemoglobin,f-Hb ELISA Kit

  • 中文名称：
    人游离血红蛋白(f-Hb)酶联免疫试剂盒
  • 货号：
    CSB-E09632h
  • 规格：
    96T/48T
  • 价格：
    ￥3600/￥2500
  • 其他：

产品详情

  • 缩写：
    f-Hb
  • 种属：
    Homo sapiens (Human)
  • 检测范围：
    Request Information
  • 灵敏度：
    Request Information
  • 反应时间：
    1-5h
  • 样本体积：
    50-100ul
  • 检测波长：
    450 nm
  • 研究领域：
    Metabolism
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%      
    Three samples of known concentration were tested twenty times on one plate to assess.  
    Inter-assay Precision (Precision between assays): CV%<10%      
    Three samples of known concentration were tested in twenty assays to assess.    
                 
  • 线性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of human f-Hb in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    　 Sample Serum(n=4)  
    1:100 Average % 92  
    Range % 87-97  
    1:200 Average % 87  
    Range % 84-90  
    1:400 Average % 94  
    Range % 89-95  
    1:800 Average % 96  
    Range % 90-101  
  • 回收率：
    The recovery of human f-Hb spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample Type Average % Recovery Range  
    Serum (n=5) 94 90-98  
    EDTA plasma (n=4) 92 88-96  
                 
                 
  • 标准曲线：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    ng/ml OD1 OD2 Average    
    1000 0.260 0.262 0.261    
    500 0.357 0.367 0.362    
    250 0.453 0.468 0.461    
    125 0.550 0.587 0.569    
    62.5 0.682 0.698 0.690    
    31.25 1.374 1.399 1.387    
    15.6 1.984 1.998 1.991    
    0 2.363 2.398 2.381    
  • 货期：
    3-5 working days

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