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  4. Human glucocorticoid receptor-β,GR-β ELISA Kit

Human glucocorticoid receptor-β,GR-β ELISA Kit

  • 中文名称：
    人糖皮质激素受体β(GR-β)酶联免疫试剂盒
  • 货号：
    CSB-E08889h
  • 规格：
    96T/48T
  • 价格：
    ￥3600/￥2500
  • 其他：

产品详情

  • 产品描述：

    This Human GR-β ELISA Kit was designed for the quantitative measurement of Human GR-β protein in serum, plasma, tissue homogenates, cell lysates. It is a Sandwich ELISA kit, its detection range is 39 pg/mL-2500 pg/mL and the sensitivity is 9.75 pg/mL.

  • 别名：
    lucocorticoid receptor-β,GR-β|lucocorticoid receptor-beta,GR-beta
  • 缩写：
    GR-β
  • 种属：
    Homo sapiens (Human)
  • 样本类型：
    serum, plasma, tissue homogenates, cell lysates
  • 检测范围：
    39 pg/mL-2500 pg/mL
  • 灵敏度：
    9.75 pg/mL
  • 反应时间：
    1-5h
  • 样本体积：
    50-100ul
  • 检测波长：
    450 nm
  • 研究领域：
    Epigenetics and Nuclear Signaling
  • 测定原理：
    quantitative
  • 测定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%        
    Three samples of known concentration were tested twenty times on one plate to assess.    
    Inter-assay Precision (Precision between assays):CV%<10%        
    Three samples of known concentration were tested in twenty assays to assess.      
                   
  • 线性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of human GR-β in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.  
    　 Sample Serum(n=4)    
    1:1 Average % 103    
    Range % 99-108    
    1:2 Average % 98    
    Range % 94-102    
    1:4 Average % 91    
    Range % 88-95    
    1:8 Average % 89    
    Range % 85-96    
  • 回收率：
    The recovery of human GR-β spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.  
     
    Sample Type Average % Recovery Range    
    Serum (n=5) 95 90-100    
    EDTA plasma (n=4) 87 83-95    
                   
                   
  • 标准曲线：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.  
     
    pg/ml OD1 OD2 Average Corrected    
    2500 2.441 2.382 2.412 2.301    
    1250 1.692 1.725 1.709 1.598    
    625 1.021 1.103 1.062 0.951    
    312.5 0.563 0.539 0.551 0.440    
    156 0.327 0.314 0.321 0.210    
    78 0.245 0.258 0.252 0.141    
    39 0.185 0.191 0.188 0.077    
    0 0.108 0.113 0.111 　    
  • 数据处理：
  • 货期：
    3-5 working days

产品评价

问答及客户评论

■ 常见问题解答
Q:

Please provide the following information for CSB-E08889h
1.What is the standard? (Recombinant full-length protein? Partial protein
(range?)?, peptide (sequence range?), native protein?)
2. If recombinant protein, then what is the host?
3. Is the capture antibody a monoclonal or polyclonal?
4. What is the host of the capture antibody?
5. Is the detection antibody a monoclonal or polyclonal?
6. What is the host of the detection antibody?

A:
Thanks for your inquiry.
CSB-E08889h
Capture antibody is mouse monoclonal antibody.
Detection antibody is goat polyclonal antibody.
Standard is CHO cell-derived recombinant protein.